FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759946
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT3196685.549843696451321No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG1763243.0612092543923155No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC1562632.712924739224986No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC1011371.7558671556990293No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC1005051.7448948306112593No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC877811.5239899818505243No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA854331.4832257107965943No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA771981.3402556204519973No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT585611.016693559279896No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC574180.9968496232429958No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG489860.850459361945407No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG444730.772107932956316No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT442920.7689655423852932No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA424270.736586766612048No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA419950.7290866962988889No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC401750.6974891778499313No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC381730.6627319075560778No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA370550.6433220033660039No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG368320.6394504392923128No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA364250.6323844008259799No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT339170.5888423259523614No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT324960.5641719557787521No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG302140.5245535288004436No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC286730.4977998057620679No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG285880.49632409748285833No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC267770.46488283049875817No Hit
GTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTT263940.45823346260537856No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC254040.4410458014710555No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC240250.4171046048001144No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT240010.4166879342271611No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA229960.3992398539847422No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA222040.38548972507728374No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG221690.38488208049172684No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG221110.3838751266070897No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG219460.3810105164180359No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA208620.3621908955396457No Hit
CATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATAT208320.3616700573234541No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT207350.3599860137577679No Hit
ATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTGGTAC206860.35913531133798826No Hit
GAGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT200450.3480067347853608No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC199230.3458886593728483No Hit
GTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT198590.3447775378449729No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA197210.34238168205049146No Hit
GAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC191990.3333190970887574No Hit
TACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACT188680.3275725154367767No Hit
GTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT186720.32416970575765813No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA186690.324117621936039No Hit
GAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC185510.32206899161901864No Hit
GGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACA183820.3191349363344726No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA183060.3178154795201205No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG179710.3119994527726475No Hit
AACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGA178870.310541105767311No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTAT175500.30469035647209197No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACAT173750.30165213354430753No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC168430.292415935843843No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA162870.28276306757042513No Hit
AAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT158910.27588800311669587No Hit
CATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAG158560.27528035853113897No Hit
AGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGAC156110.27102684643224084No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA148530.2578670008364662No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA143330.24883913842247826No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA141940.2464259213541238No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC140380.24371756262992744No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA139590.2423460219939562No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA139370.24196407396874903No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG137850.23932516034004483No Hit
CTGTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG136670.23727653002302454No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG135620.23545359626635387No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA134680.23382163652228685No Hit
ACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTAT133770.23224176059983898No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT133350.2315125870971707No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT130050.225783366719063No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC121170.21036655551979136No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA120020.20837000902439018No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT119680.20777972571270634No Hit
CATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGC113960.19784907705731963No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC113800.19757129667535075No Hit
GTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT112880.19597405947902985No Hit
CATTATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTG110340.19156429591527419No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT110250.19140804445041673No Hit
CTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATA103440.17958501694286716No Hit
TAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATA103400.17951557184737496No Hit
CTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG102990.17880375961857975No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG98610.17119952166218225No Hit
AAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACA93790.1628313876553704No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG92470.1605396995041273No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG90950.15790078587542314No Hit
CTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAAC90810.1576577280412004No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA90250.15668549670430937No Hit
TAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACT87760.15236253950991901No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA87520.15194586893696574No Hit
GTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATT87250.15147711454239327No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA87190.15137294689915495No Hit
GAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGT86350.14991459989381845No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA85810.14897709110467355No Hit
CAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACC84990.14755346664708316No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTT82140.14260550359326285No Hit
AGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTA82120.14257078104551674No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT80960.14055687327624253No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC80530.1398103384997012No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG80440.13965408703484372No Hit
GCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG79390.1378311532781731No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT79350.13776170818268088No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCATGATAACCTATACAT79070.13727559251423538No Hit
TGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAG76190.13227554563879593No Hit
TGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTG75300.13073039226409414No Hit
CTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAG75060.13031372169114086No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA74540.12941093544974205No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG73360.12736230513272173No Hit
CCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATT73010.12675466054716486No Hit
TTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCT72550.1259560419490044No Hit
ATGTATAGGTTATCATGCGAACAATTCAACTGTCGCCAGTTCATTGGTAC72090.12515742335084393No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC71000.12326504449868107No Hit
GTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGT69940.12142474946813736No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT69170.12008793137991225No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC66000.11458440756215423No Hit
CAGTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCG65700.11406356934596262No Hit
CAATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA65130.11307397673519855No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT64910.11269202870999137No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA64510.11199757775506923No Hit
CTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTC64430.1118586875640848No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA64200.11145937826500457No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC63280.10986214106868362No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCA62480.10847323915883933No Hit
GTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACAT62080.10777878820391719No Hit
GTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTA61250.10633780247245374No Hit
CTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA60850.10564335151753158No Hit
TGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTG60460.10496626183648249No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT59810.10383777903473401No Hit
TAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAGTA58690.10189331636095199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)