FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7981707
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGACAGCAATTGGAGAAGACAAGACCAGCAGCAGGCTTGGGGACA618310.77465885430272No Hit
CTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAAC526190.6592449459745892No Hit
TCCCAGAGGAGTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAG432720.5421396701231954No Hit
GTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAGAAAGTAGGAG413960.5186359258740016No Hit
CTCTAGGCCTCAGCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTG408240.5114695390346953No Hit
GCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGAGTGGTG363300.45516579348252195No Hit
GTCCTGCACCACTCAGCCACCTCCCTGCATCACAGGTGTGACCACAGGGA334300.4188327133531712No Hit
GGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACCAAAGGGGCTGGT329560.4128941340492704No Hit
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT324020.4059532628797323No Hit
GTCTTGGGACGCTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCC319550.40035295708048413No Hit
TCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTTCCCAGC291250.364896882333566No Hit
AACCAAGGGTGTCCAGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAAT284180.3560391279709967No Hit
GTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATTGCTGT276990.3470310298285818No Hit
CACCCAGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACC265860.33308664424790335No Hit
TGCTTAGCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGA261060.32707289305407977No Hit
CCTCTACAGCGTCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTTTCT255980.32070833970728313No Hit
GTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAACAT254450.3187914565142519No Hit
GTTTGGGTGTGCCAGACTCAAACCAAGGGTGTCCAGGCAAGACAGGGAGG249920.3131159788250809No Hit
GTGTATGAGGGGGTACTCCAATGCACCGTCAACCCTGTCCTTGGTATTTA249360.3124143745191348No Hit
GATCAGGAAGGGATGACATGCTTAGCCTTATCCCTGTGGTCACACCTGTG212500.2662337768098979No Hit
GCTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAAT211680.26520642764761976No Hit
GTCCAGGGTTGGTCCCTTACTCGACCCTTCGAACATTTAAGTACAGGAAG211420.2648806827912876No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC205880.25793981162174956No Hit
ACCCTGGACTTGCCAGACTACTGCTAAGTTTTTTGTTTGTTTGTTTCAAG198850.24913217185246211No Hit
GAACATGACCAGCCCCTTTGGTTGGGTTCCCAGCAATGAGCCCAGCCTCT195100.24443392873228745No Hit
ACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGAGTCCAGGGCCAAGGGAAA185990.23302033011234313No Hit
CACCTGTGATGCAGGGAGGTGGCTGAGTGGTGCAGGACAGCAATTGGAGA183130.22943713669268995No Hit
GCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGCA178710.22389947413504405No Hit
GTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGAG177560.2224586795781905No Hit
CCTGTAGGCCCAGCTACTCGGGAGGCTGAGGCCGGGGAATGGCGTGAACC171340.21466586032286078No Hit
GCCTGAAAGGCTTCCCAGAGGAGTCCAGGGCCAAGGGAAAGTGAGACTGG168390.21096990906832336No Hit
CTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCCCCGGCCTCAGCCTCCC161700.20258824334193176No Hit
CCTGTAATCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCA160570.2011725060817191No Hit
TCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTC158050.1980152867049617No Hit
GGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGT152930.19160061876488324No Hit
GGTCACACCTGTGATGCAGGGAGGTGGCTGAGTGGTGCAGGACAGCAATT150600.18868144370621473No Hit
GCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATT143550.17984874664028636No Hit
GCCTACAGGCGCCCGCCACCACGCCCGGCTACTTTGTTCTATTTTTCCTA143430.17969840286044075No Hit
GGCTGGAGTGCGGTGGCGCCATCTCGGCTCACTGCAAGCTCCGCCTCCCG142710.17879634018136722No Hit
GGCTTGGGGACACCCAGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATT139660.17497510244362516No Hit
CTCCAGGGGCACTAAGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAA138540.173571893831733No Hit
CTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTCCAGACCAT137430.1721812138681613No Hit
AGACAGGGAGGTGGCATGGGAAGGGTGGAATGGGCCAGATCAGGAAGGGA133740.1675581426379094No Hit
ATCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTTCCCAG133660.1674579134513457No Hit
GGCCTAGGATGGGGGCTTGGATAGGCGGTGCCTCTACAGCGTCCCAAGAC132880.16648067888234935No Hit
GGGTGGAATGGGCCAGATCAGGAAGGGATGACATGCTTAGCCTTATCCCT132720.1662802205092219No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT131760.1650774702704572No Hit
ATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACCAAAGG127820.16014118283219367No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA126660.15868785962701965No Hit
GAACAACTCCAGGGGCACTAAGTGGAAGGTGTGCCAGAACTCAGAGCAGC126220.15813659910091912No Hit
AGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACC122890.15396455921020405No Hit
CCTGTGATGCAGGGAGGTGGCTGAGTGGTGCAGGACAGCAATTGGAGAAG122790.15383927272699938No Hit
ATCCAAGCCCCCATCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGG121070.15168434521587926No Hit
GTCTCAGTGGGGCCAGCAAAAGCAAAACTTGTTGGGGTTCTGCTGAGGAG119340.14951688905643867No Hit
GTGCCTCTACAGCGTCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTT118900.14896562853033818No Hit
GCTTAGCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGAG118470.14842689665255815No Hit
GTATTTATGTAAGGAATTCCAGCATTTGCAAATACATTGCTTTCCTAGGA117720.14748724802852323No Hit
GTATGAGGGGGTACTCCAATGCACCGTCAACCCTGTCCTTGGTATTTATG117340.14701115939234552No Hit
GTCTGGAGCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAGAGTGC115420.14460565891481608No Hit
GTCTCGCCCTGTCGCCCAGGCTGGAGTGCGGTGGCGCCATCTCGGCTCAC113420.14209992925072296No Hit
GTCATGTTCCCCTCCAGGCCTAGGATGGGGGCTTGGATAGGCGGTGCCTC113000.1415737260212634No Hit
CCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATTGCTGTCCTGCACCAC111990.14030833254089634No Hit
AGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGA111190.13930604067525906No Hit
ACTCCAGGGGCACTAAGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAA109150.13675019641788405No Hit
CACTGAGACAGTGAGGCCCGCTTCCCCACAGGGGGGCCTTGGTCAAGGGT107760.13500871430133932No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG107390.13454515431348207No Hit
TCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTTTCTCTCAGATGCTC105200.13180138033130007No Hit
CTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAATA104900.13142552088168608No Hit
CAGTTTGGGTGTGCCAGACTCAAACCAAGGGTGTCCAGGCAAGACAGGGA103890.13016012740131905No Hit
TGTCTGATTTGGGGACAGTTTGGGTGTGCCAGACTCAAACCAAGGGTGTC103330.12945852309537298No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC103300.12942093715041156No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT103160.12924553607392503No Hit
GTCCAGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAATGGGCCAGATC101620.12731612423257332No Hit
CTGTAGGGGGAAGGGAGGCAGCGGGGAAGCCACAAAAGCCTACAGCAGGC101390.12702796532120258No Hit
GGCATGGGAAGGGTGGAATGGGCCAGATCAGGAAGGGATGACATGCTTAG101380.12701543667288212No Hit
AGCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGC100540.125963030213963No Hit
GTAGGGGGAAGGGAGGCAGCGGGGAAGCCACAAAAGCCTACAGCAGGCCG97960.12273063894728284No Hit
CCTCAGCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTC97510.12216684977286187No Hit
GGCCAGATCAGGAAGGGATGACATGCTTAGCCTTATCCCTGTGGTCACAC93660.11734332016948253No Hit
CTCCAATGCACCGTCAACCCTGTCCTTGGTATTTATGTAAGGAATTCCAG92790.11625332776560203No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG92090.11537632238316942No Hit
TTCTAGGACTCTACAAGCTGGCAAAGCTGGCCAGAAGCAATTTCTGAAAC91400.11451184564905728No Hit
AAAGCAGGGAGGTGAAATTGTGCATGTCTGATTTGGGGACAGTTTGGGTG90640.11355966837670187No Hit
GGACAGCAATTGGAGAAGACAAGACCAGCAGCAGGCTTGGGGACACCCAG90610.11352208243174049No Hit
GGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAATAGAACAAAGTAGCCG89470.11209381652320738No Hit
GGTGAAATTGTGCATGTCTGATTTGGGGACAGTTTGGGTGTGCCAGACTC88110.11038992035162402No Hit
GGCTAAGCATGTCATCCCTTCCTGATCTGGCCCATTCCACCCTTCCCATG86330.10815982095058112No Hit
GTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGAGTCCAGGG83040.10403789565314789No Hit
AGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAATGGGCCAGATCAGGA82630.10352422107200879No Hit
GGAATACCGGGTGCTGTAGGCTTTTTCTTTGGCTTTTTGCTGTTTCTTTC82130.10289778865598549No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT81970.10269733028285805No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC81570.10219618435003941No Hit
TCACAGGTGTGACCACAGGGATAAGGCTAAGCATGTCATCCCTTCCTGAT81040.10153216598905472No Hit
CTGTAGGCTTTTTCTTTGGCTTTTTGCTGTTTCTTTCCTTTTCTTCCAGA80860.10130665031928635No Hit
GGATAAGGCTAAGCATGTCATCCCTTCCTGATCTGGCCCATTCCACCCTT80660.10105607735287703No Hit
AAGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGG80300.10060504601334026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)