FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5982974
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT223160.3729917596165385No Hit
GTGTACGGAGCGCGGCTCATGGTTCCAGCTGGCCGGGGGCTCATCGTGGT195380.3265600017650085No Hit
CCGTACACCGAGCACAAGTAGTGGCCTCTGAGTCCAACATTGTTGATATC188130.31444228238330973No Hit
GTCTATAACTGTGATTATTTAGCTTCTCTCTTATTCAGCCTTCTCAGGGT158950.2656705511339344No Hit
GTTATAGACAACACAGAAGATTCTAGGTGGCACGTTCTTAAAAATCGAGG155910.2605894660414704No Hit
GTCTAGTCTCCAGGTAAATATCATTGCTCCATGCATGCACTTTAGCCATA153250.2561435165855643No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC137160.2292505366060424No Hit
GCTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAAT133240.22269861109207564No Hit
TTCCTAACCTCCCCCTCTGACCCTGGAATGCTCATTCCCACTCACCGTTC132140.2208600605651972No Hit
ATCTTACTTTCTGGTACAAATGGAAGATTTGATCCATAAAGAAATTCAAA130760.21855351535874967No Hit
GCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGCA125140.20916019357597074No Hit
GTCCGTAGCCAGCCTGTCGCACGCAGCTTTGCCCACGCCATAGGGGACAT124810.20860862841790723No Hit
GTCTTAAAGCGTTACCATTAAAAAGGAATTATCCTACTTAGGAAAAAAGG124730.20847491565231607No Hit
GTACAATGCCTTGCCGGTGGTAGGCCTCAGTAAATGTAGATTTTAAACCT118370.1978447507878189No Hit
CCTGTAATCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCA109890.18367119763515605No Hit
TCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTC107490.17965981466742123No Hit
GGTTAGGAATGTGGAGCTTGAATCTAAGTTTAAAGAGTACTGGGACTCCG106870.17862354073408976No Hit
CCTGTAGGCCCAGCTACTCGGGAGGCTGAGGCCGGGGAATGGCGTGAACC103000.17215518569861746No Hit
GGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGT100770.16842794235776387No Hit
CTTTAAGACAGGGAAACATTGGTGGCTGTGTCTAGAGTGCAATGAATCTG98930.16535254874916722No Hit
CCATAGGGGACATTGAAGAGATACTGTAGCCCCCCAAGGGAGGAGATGAC95310.15930204610616727No Hit
CTCCAGATCCTTCTCAATATCCAAAAGTAAATAAAATGCAGAGATAGTGG95030.1588340514265982No Hit
GAATAAGAGAGAAGCTAAATAATCACAGTTATAGACAACACAGAAGATTC93460.15620993840187172No Hit
CCACAGGAACACACTGGCCCCCTCGAGATTGTGCCTGGTGAGGGACAGAG92070.1538866790997253No Hit
CTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCCCCGGCCTCAGCCTCCC91360.1526999783051038No Hit
AGACTAGACAGTGCCATTCAACTGGATATTAAAGGAACAGTGAGGAGAGT90630.15147984931908445No Hit
CTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTCCAGACCAT90110.15061071634274192No Hit
CCCCTATGGCGTGGGCAAAGCTGCGTGCGACAGGCTGGCTACGGACTGTG89310.14927358868683033No Hit
GTCTAGAGTGCAATGAATCTGCATGTATTGTCTACCTGCTTGAACAGTGA85020.1421032416320044No Hit
GTACTGGGACTCCGGCATAGGCGTTGTTGACCAGCACATCCAGACGCCCT83890.14021454881802928No Hit
GAATTTGAGTAATCAAGGCTTTTCTCTCTCAGGTTTTTCGGACAATTTGA83750.13998055147824476No Hit
CTCCGTACACCGAGCACAAGTAGTGGCCTCTGAGTCCAACATTGTTGATA83460.1394958427029768No Hit
GCCTACAGGCGCCCGCCACCACGCCCGGCTACTTTGTTCTATTTTTCCTA83230.13911141850190223No Hit
GGTAAGGACAGTTCTGCAGGCCCAGGGAAAAGCAGAGAGCTAGTCATGAA82740.13829242781265638No Hit
GTAAATACACTTGTTTCTTTGACCCTCCTGGGTGAGAGTCTTGGAAGCAT81250.13580202755352105No Hit
GGCTGGAGTGCGGTGGCGCCATCTCGGCTCACTGCAAGCTCCGCCTCCCG80350.13429775894062052No Hit
ACTATAAACTCATATCCCCTCTGTCCCTCACCAGGCACAATCTCGAGGGG77010.1287152509771896No Hit
AGATGTTACTTTGTATTCCAGGTGAGAAAACCACCGGGAATGAAGAAAGA75010.12537243183741062No Hit
AGCCAGGAGAGTGAAGTGCAAAGCTTGTTTGAGCAAGTCGATCGGGAACA74710.12487100896644379No Hit
GGCTTATAGGAAGTGTAGCCAGGAGCCAGAGGTCTAAGAAGCTTCCTAGG73790.12333331216214545No Hit
GTCTTACACGACTTGCCAAAGAATTTGGGACTTTATTCTGAGGATAACAG73100.1221800395589217No Hit
GATCTAAGAAGGACCTAAGAGCTCCCTAATCAATTGGTCCTCATTGTTCC71650.11975649568258194No Hit
GGATAAGAGATTGCTTCCAGTTTTGAGGAATTATTGAGTCATTCAATTCT71390.11932192919441069No Hit
GTCCCTCACCAGGCACAATCTCGAGGGGGCCAGTGTGTTCCTGTGGTGTG71200.11900436137613167No Hit
GTCTCGCCCTGTCGCCCAGGCTGGAGTGCGGTGGCGCCATCTCGGCTCAC70640.11806837201699355No Hit
GCTAAGAGCCATGTAAATACACTTGTTTCTTTGACCCTCCTGGGTGAGAG69900.11683152893527535No Hit
CAATGGGACAGGGCACCACTGAAACAGGCTAAGGTGCCCAGAATTGTTTC68260.11409041724065656No Hit
CTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAATA68190.11397341857076432No Hit
GTGTTAGGTTTGCACTTTGCAGAGAAAATGAATATGTCATAATCCCAATT67810.1133382829342063No Hit
TGTAAATACACTTGTTTCTTTGACCCTCCTGGGTGAGAGTCTTGGAAGCA67740.11322128426431405No Hit
GGCTACGGACTGTGCCCGGGAGCTGCGGCGCCATGGGGTCAGCTACGTGT67300.11248586405356266No Hit
GTGTAACCCTGAGAAGGCTGAATAAGAGAGAAGCTAAATAATCACAGTTA66900.11181730022560687No Hit
AGCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGC66780.11161673107722013No Hit
CGGTGTACGGAGCGCGGCTCATGGTTCCAGCTGGCCGGGGGCTCATCGTG65730.10986175102883616No Hit
GTCCTGGGATTGAGCCAGCATCGGGCTCCCTGCTCAGGGGGAAACTGTTT65020.10867505023421462No Hit
ATGTTACTTTGTATTCCAGGTGAGAAAACCACCGGGAATGAAGAAAGATA64630.10802320050195771No Hit
CTATAAGCCAGTTCCTGAGGTTCCCAAAACCTCAGACTGGGCTGTTTTGG63370.10591722444389697No Hit
CTCCTGGCTTGAATCACACACCACAGGAACACACTGGCCCCCTCGAGATT63100.10546594386002679No Hit
GCCTTATTTGTGCCTTGCCTTCCACTAGCAGAGTACAATGCCTTGCCGGT62100.10379453429013731No Hit
CTGTAGCGTTGTGCAAGGTCACAAGATGGCAGCACCTTCCCACTCAGGCT61830.10334325370626717No Hit
GTCTTGGAAGCATTGGAATTTGTTAAAGGGAAAAAAGGATCTGTCTGAAG61730.1031761127492782No Hit
TCTCAGAGGTGAGGCTTAAACATCTGAGAAGTGCCTGTCGCAGCGCCAGC61640.10302568588798815No Hit
CTTATGAAGAACATTAAGAAGCCATTCTGGGAAGCCCCTGCCTCCATCTG61290.10244069253852682No Hit
GTTTATAGTTGCACATGAATGTGTGACATGAGGCAACTCTCTTCAACTCC61260.10239055025143015No Hit
GTCCTGGGATTGAGCCCCACATTGGGCTCTCTGCTCAGCAGGGAACCTGC61190.10227355158153788No Hit
CTTCATAAGTGCCTGGAAGGCACAGGAGATGTGGTGAGTTGGACAATTTG61140.10218998110304339No Hit
GAGTAGAGATGATCCCATGCAAGCTGGTGAAATGAGGTGTTATCCAGTGG61120.1021565529116456No Hit
GATCTGAAAGGTCTTACACGACTTGCCAAAGAATTTGGGACTTTATTCTG60900.10178884280626992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)