FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA90.28133791809940606No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT90.28133791809940606No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC80.25007814942169426No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC70.2188183807439825No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC60.1875586120662707No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC60.1875586120662707No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC60.1875586120662707No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC60.1875586120662707No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC50.15629884338855893No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA50.15629884338855893No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT50.15629884338855893No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT50.15629884338855893No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC50.15629884338855893No Hit
GAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGG40.12503907471084713No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG40.12503907471084713No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA40.12503907471084713No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT40.12503907471084713No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG40.12503907471084713No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT40.12503907471084713No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA40.12503907471084713No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA40.12503907471084713No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT40.12503907471084713No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC40.12503907471084713No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA40.12503907471084713No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG40.12503907471084713No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT40.12503907471084713No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA40.12503907471084713No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA40.12503907471084713No Hit
CCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTT40.12503907471084713No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC40.12503907471084713No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT40.12503907471084713No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG40.12503907471084713No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG40.12503907471084713No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.12503907471084713No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT40.12503907471084713No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC40.12503907471084713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)