FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_A11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5224752
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG299020.5723142457287925No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT195050.373319154669925No Hit
ATCTAAGACTGTCTCTCTTTTAATCACACACACACATGCACACACACAGA193160.3697017580930157No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT160340.30688537944001937No Hit
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA160120.30646430682260134No Hit
GTCTTAGATTCTCCTTCATGAGTATGTCCCTTAGAGGGATAAATGATGAA119070.22789598434528568No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC115470.22100570515117274No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC113990.21817303481581518No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA108110.20691891213209737No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT97560.18672656616046082No Hit
GTAGGAGACAGAGTGGCATTTTGTACAATATTAAATTCAGGTTCTCAATA95780.18331970589226051No Hit
CAATAATGGTCAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACA95320.18243928132856832No Hit
ACCTACAACAGTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATC93610.17916639871136467No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT93210.17840081213424103No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG91970.17602749374515766No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC91160.17447718092648223No Hit
ACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACT88950.17024731508787402No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT84070.16090715884696538No Hit
GTCCTGGGGAGACACCAAACTGTGTTTTAGTGAGCTTCCTAAGGCTTGAA82860.15859125945116628No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT82770.15841900247131346No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC82650.15818932649817638No Hit
GTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCC81910.1567729913304976No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC80160.1534235500555816No Hit
ATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGAAA79440.152045494216759No Hit
AGTTAGGGTTGCTTTGGGCTCCCAGATCACCAACAGATTTCATCATTTAT79170.15152872327720052No Hit
GAACTATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTT79040.15127990763963534No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA78390.1500358294518094No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA78160.14959561716996328No Hit
CTCTAAGGGACATACTCATGAAGGAGAATCTAAGACTGTCTCTCTTTTAA78150.14957647750553518No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT77930.14915540488811718No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG77860.14902142723712053No Hit
ATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTCCT77630.14858121495527443No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT77210.1477773490492946No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGGCACACCCCAAGT77180.1477199300560103No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA76020.1454997289823517No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG75970.14540403066021124No Hit
GTTCTGGAGACTGAATAGGAAAACCAGAAAAGAGAACATATCAGGCAGAG75710.14490639938508085No Hit
GAATAATGTCCCTCCCCAAAAATGATAGCCCAATTCCTAGAAACTGTGAA75390.14429393012338193No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA74910.14337522623083354No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA74100.14182491341215814No Hit
ATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTTCAGTA74100.14182491341215814No Hit
TTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTGCTCCT74060.14174835475444578No Hit
GAGTGATACAATCCCACCTGCGTGTTAGAAAGGATTTTCAAGTGAAGGTT73990.14161437710344912No Hit
CAATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTC71420.1366954833454296No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA69490.13300152811080795No Hit
CATCTGGATGTATAAAAAAATGCTCATGATCTCAACAGAGGAATGCTCTT69440.1329058297886675No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA69250.13254217616453376No Hit
ATCTTAAGGTTAGCTGATTGACAACCTTAACTCCATCTTTGACCTAATAC68890.13185314824512245No Hit
CTTCTACCCTGTAAGTATCTCTCCGTTCATTGCTGTTAACTGTCCCCCAG68630.13135551696999206No Hit
ATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCCTGATATGTTCT66610.12748930475551756No Hit
GGGTAGAGGTGGGGAAGGCAGAGATCTGAGAACTCTTTAGGAAGCAGAAT66480.12724048911795238No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG66200.12670457851396583No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA63190.12094353952111028No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT63180.1209243998566822No Hit
GTTCATATGTTGGGGACATCACCCCCATTCTGAAGAAGTAGAAGGTGGGT62930.12044590824597992No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG62610.11983343898428099No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT62360.11935494737357868No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC62040.11874247811187975No Hit
GTCTAGGGAATATCCTCTAAGGAGTAAGTCATGTTTGTTTAATCGGATCA61860.1183979641521741No Hit
GAACAATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAG61820.11832140549446175No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG61800.11828312616560556No Hit
GTAGTTGGGTGTTTGCTACTTGCACTGAACTATCCTGATTGATCCTCTAA59920.11468486925312435No Hit
ATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAAT59610.11409153965585353No Hit
CATATGAACAAGTACTGAATTTTGTCTATGCTCAAATGAGGTGATGTGTG59300.11349821005858268No Hit
GGTGTACATAGAATGCTCCACAAATATTGGCTGGAAGGAAATGGTAGCCT58220.1114311263003488No Hit
GTCTAGGAAGTGCAGGGAAATAGAGAAATCAGAAGACCTGAGTTGAACTC57950.11091435536079033No Hit
TAATGGTGGTGTGTCCCTACTTTTAGATAATAACGCACAATAGAGAATGG57910.11083779670307797No Hit
CACATGCACACACACAGAAAGGGAGAAAATTAACCCAAAGTAAAAGCAGA57850.1107229587165094No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA57660.11035930509237567No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA57360.10978511515953293No Hit
GTCTGGAACAGTGGCAACCATATTGTGTCAGTGAAATGAGGCATCATCCA57160.10940232187097111No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT57060.10921092522669018No Hit
CTCATGGACCAAGCAACGGCACTCTGGGGAACAAACAGGACACAAAAACT57020.10913436656897783No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT57020.10913436656897783No Hit
GGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAATAATGCAGTTG56520.10817738334757325No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA56350.1078520090522957No Hit
GGGTATGCTGGAAGCATTTTTATTTCAGTTTAGTATCCATTTCGGGTAGG55900.10699072415303157No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA55430.10609115992491126No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT55300.10584234428734607No Hit
ATGTACACCAGGCCTTGTGCTGATTGCAAGGGAGACAGAGACCCTGCCCT55270.10578492529406182No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA55110.10547869066321233No Hit
CATCAGGAAAGACTACTTTGGGGAGAAAGAACAATAATCACCTGATGTAA54940.10515331636793478No Hit
AGACTATACTCTAAGTGGTATTAGGTCAAAGATGGAGTTAAGGTTGTCAA54510.10433031079752685No Hit
GTAAGTATCTCTCCGTTCATTGCTGTTAACTGTCCCCCAGCTTTGTTGGA54450.1042154728109583No Hit
ATATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGG54300.10392837784453694No Hit
GCCTGATATGTTCTCTTTTCTGGTTTTCCTATTCAGTCTCCAGAACCCAT54220.10377526052911219No Hit
ATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAGTTGAT54060.10346902589826272No Hit
TATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGGC54040.10343074656940655No Hit
CTCCAGAACCCATCCACATGCCGGTTCTCTGAGTTCCAGAATTTCCCCAG53420.10224408737486489No Hit
GGTGATTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTG52560.10059807623404901No Hit
AGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACTACTTT52540.10055979690519282No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT52390.10027270193877144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)