FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_F8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_F8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189588
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT22161.168850349178218No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA19501.0285461105133236No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC10590.5585796569403126No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT9950.5248222461337215No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG9730.5132181361689558No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC9260.4884275376078655No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT8670.45730742452053924No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC8220.4335717450471549No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT8200.4325168259594489No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA8110.42776969006477206No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT8000.4219676350823892No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC7270.38346308838112114No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT7160.3776610333987383No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC7100.37449627613562037No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC6480.34179378441673525No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG6420.3386290271536173No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG6280.3312445935396755No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA5980.3154208072240859No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC5840.30803637361014413No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT5780.3048716163470262No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC5700.30065193999620227No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG5590.2948498850138195No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA5540.2922125872945545No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC5280.27849863915437684No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT5280.27849863915437684No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG5250.2769162605228179No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT5200.27427896280355296No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC5060.26689452918961115No Hit
CATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAAC5020.2647846910141992No Hit
GGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAA4990.26320231238264025No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC4980.2626748528387872No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT4930.2600375551195223No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA4900.25845517648796335No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA4880.2574002574002574No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT4660.24579614743549172No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA4650.24526868789163872No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA4630.24421376880393275No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA4530.23893917336540288No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT4490.23682933518999094No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT4490.23682933518999094No Hit
GATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAA4370.23049982066375507No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA4330.22838998248834314No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA4330.22838998248834314No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC4190.22100554887440135No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC4100.21625841297972445No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA3990.21045635799734158No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA3940.20781906027807667No Hit
ACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTA3930.20729160073422367No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAAATTAC3920.2067641411903707No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA3920.2067641411903707No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA3840.2025444648395468No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT3780.19937970757642887No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG3760.1983247884887229No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT3680.19410511213789902No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATTTCGTCA3580.18883051669936915No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3550.18724813806781018No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG3530.1861932189801042No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTATT3520.18566575943625124No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG3430.18091862354157437No Hit
CACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCG3390.1788087853661624No Hit
GTCCAAAGTTCTTTCATTTTCCAATAGAACCAACAGTTCGGCATTGTAAG3380.17828132582230943No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA3370.17775386627845643No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT3370.17775386627845643No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG3320.1751165685591915No Hit
TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAA3310.17458910901533853No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT3280.17300673038377956No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT3250.17142435175222062No Hit
GGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCA3210.16931451357680866No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC3200.16878705403295566No Hit
GCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAA3170.16720467540139672No Hit
GTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAA3130.16509483722598478No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG3090.16298499905057282No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA3090.16298499905057282No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3050.16087516087516088No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG3050.16087516087516088No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA3050.16087516087516088No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG3040.16034770133130788No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT2990.15771040361204294No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTCTCTTTTTTAACTAGCCA2970.15665548452433697No Hit
GGTAAATCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTT2950.155600565436631No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA2930.15454564634892504No Hit
GAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAAGTTGA2930.15454564634892504No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT2900.1529632677173661No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC2900.1529632677173661No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA2860.15085342954195413No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG2860.15085342954195413No Hit
GTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGG2820.1487435913665422No Hit
ATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATC2790.14716121273498323No Hit
GAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGG2790.14716121273498323No Hit
GGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCC2750.1450513745595713No Hit
ATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGA2740.1445239150157183No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG2690.14188661729645338No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT2690.14188661729645338No Hit
GGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA2660.1403042386648944No Hit
CATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGG2610.13766694094562948No Hit
GTCCAAATGTCCAGGAAACCATCATCAACTTTTTTATTTAAATTCTCTAT2610.13766694094562948No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC2580.1360845623140705No Hit
GTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATA2580.1360845623140705No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA2570.1355571027702175No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA2560.13502964322636454No Hit
GTCCAGGAAACCATCATCAACTTTTTTATTTAAATTCTCTATTCTTTTTT2550.13450218368251154No Hit
ATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAAT2520.1329198050509526No Hit
GTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAAT2470.13028250733168764No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA2470.13028250733168764No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC2450.12922758824398167No Hit
AATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGC2440.1287001287001287No Hit
GAATGCCATTGACGAAATTACTAACAAAGTAAATTCTGTTATTGAAAAGA2410.12711775006856973No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG2400.12659029052471676No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA2390.12606283098086377No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT2380.1255353714370108No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA2370.1250079118931578No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT2330.12289807371774586No Hit
GAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACA2320.12237061417389287No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT2290.1207882355423339No Hit
GAACCAACAGTTCGGCATTGTAAGTCCAAATGTCCAGGAAACCATCATCA2280.12026077599848091No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2270.11973331645462792No Hit
AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAAT2270.11973331645462792No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA2260.11920585691077494No Hit
GGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAA2250.11867839736692196No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2240.11815093782306897No Hit
ACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTG2240.11815093782306897No Hit
TTGTAAGTCCAAATGTCCAGGAAACCATCATCAACTTTTTTATTTAAATT2240.11815093782306897No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT2230.11762347827921599No Hit
GTTCGGCATTGTAAGTCCAAATGTCCAGGAAACCATCATCAACTTTTTTA2220.117096018735363No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA2210.11656855919151002No Hit
GCTGTGGAGAGTGATTCACACTCTGGATTTCCCAGGATCCAGCCAGCAAT2200.11604109964765702No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2160.11393126147224508No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT2090.11023904466527416No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA2080.10971158512142118No Hit
GTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAA2080.10971158512142118No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT2060.10865666603371521No Hit
CTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCA2040.10760174694600924No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC2030.10707428740215626No Hit
GTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAAGTTGATG2030.10707428740215626No Hit
AAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAATACAT2030.10707428740215626No Hit
ACGGGAAACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAA2020.10654682785830327No Hit
GGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTG2010.10601936831445029No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTT2010.10601936831445029No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTA2000.1054919087705973No Hit
TGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAA2000.1054919087705973No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC2000.1054919087705973No Hit
CAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCAGTTCA2000.1054919087705973No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT1990.10496444922674432No Hit
TCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATT1980.10443698968289133No Hit
GTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACC1970.10390953013903834No Hit
GTGTTACCCAGGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGA1970.10390953013903834No Hit
CTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGCTCCAGCA1960.10338207059518535No Hit
GAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTC1940.10232715150747937No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA1920.1012722324197734No Hit
GACCTGAAGAGCACACAGAATGCCATTGACGAAATTACTAACAAAGTAAA1910.10074477287592042No Hit
ACCCAGGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCA1910.10074477287592042No Hit
CTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAA1900.10021731333206743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)