FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_E7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_E7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4961073
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA219120.4416786449221772No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC208830.42093716419814825No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT188650.38026047994052903No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC177260.35730173694279443No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC160560.3236396642419896No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT156600.31565752005664904No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG138870.2799192835904652No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT134810.27173557010751503No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC133210.2685104613457613No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT132940.26796622424221533No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG128740.25950031374261173No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT126850.2556906540177901No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA126020.25401762884763035No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC123260.2484543162336051No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT121740.24539046290993904No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC121070.24403994861595465No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA119810.24150017546607358No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC118210.2382750667043198No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA115040.23188531997009515No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC114410.23061543339515464No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT113130.22803534638575162No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC110820.22337909561096964No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA109250.22021445763849876No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA105350.21235325503172398No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA100330.20223447629172156No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA99650.20086380506797621No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT98490.19852560121570473No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC95270.19203506983267532No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA93180.18782227151263445No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG91400.1842343380151834No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC90700.18282335293191615No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT88870.17913463478566027No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA85650.17264410340263084No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC83790.1688949144670921No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA83180.16766534175167347No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT82530.16635514131721102No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC81900.16508525474227048No Hit
CATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAAC80080.1614166935257756No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAAATTAC74750.15067304996318337No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG73630.14841547382995574No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG73210.1475688827799954No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT71840.1448073834027437No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA71650.14442440173728546No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT71310.1437390661254128No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT70640.1423885518314284No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG70590.1422877671826236No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA69550.14019144648748366No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC68060.1371880639531005No Hit
GAATGCCATTGACGAAATTACTAACAAAGTAAATTCTGTTATTGAAAAGA66760.13456766308417553No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC66390.13382185668302No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA64850.130717689499832No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA64530.13007266774748125No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC64200.12940748906536953No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA63580.12815775942018995No Hit
GTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAA63360.1277143069654488No Hit
GTATGACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACATAATC63180.1273514822297515No Hit
TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAA62660.12630332188218152No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT61730.12442872741441216No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG61680.12432794276560737No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT61650.12426747197632447No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA61250.12346119478588605No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG61100.12315884083947164No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG61040.12303789926090586No Hit
GCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAA60180.12130440330146322No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA60130.1212036186526584No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA57180.11525732437317493No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT56760.11441073332321455No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA55550.11197174482213829No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT55520.11191127403285539No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT55180.11122593842098272No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT55080.11102436912337312No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT54880.11062123052815388No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA53780.10840396825444819No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA53700.10824271281636048No Hit
GAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGG53590.10802098658898993No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT53480.10779926036161935No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA53430.10769847571281456No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA53410.10765816185329263No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG53070.10697282624141996No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA52830.1064890599271569No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA52620.10606576440217672No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC52610.10604560747241575No Hit
TGTTAACATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGTAATGACG52410.10564246887719653No Hit
GATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAA51780.10437258230225598No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT51180.10316316651659832No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC51140.1030825387975545No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA50440.10167155371428721No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA50360.10151029827619952No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG50330.10144982748691664No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT49700.10017994091197609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)