FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_E2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_E2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852996
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC968851.2337329600066014No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC666200.8483386468043533No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA664650.8463648778122388No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC663990.8455244342414028No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT613470.7811922990919644No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC585970.7461738169738021No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA574630.7317334683476218No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA544240.693034862108678No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC526610.6705848315725617No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA500040.6367506108496681No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG399440.5086466362646817No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA397440.506099837565179No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT385300.4906407694591975No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT363470.46284246165412535No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT361100.4598245051952147No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC358800.45689568669078656No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA351360.4474215955286365No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG335090.42670338810818187No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA326330.41554840980436003No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT317250.4039859437086177No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC310670.39560697598725375No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA309850.3945627885204577No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA303610.3866167765780092No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA298630.38027524781624744No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG290200.3695404912978435No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT286140.36437048993785304No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG284980.3628933466921414No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT276090.3515728264728519No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA271680.34595713534044836No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT270940.3450148198216324No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT266610.339501000637209No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA251400.32013259652749093No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG246530.3139311416942018No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT245400.31249220042898274No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT239130.3045079865060418No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT236430.3010698082617131No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC231450.29472827949995134No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA230290.29325113625423976No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC227540.28974928804242356No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC224870.2863493117785874No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA224130.2854069962597714No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG222790.2837006411311046No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG215200.27403554006649183No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC210120.2675666713697549No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC209570.26686630172739173No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA209340.2665734198769489No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG208810.26589851822158067No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC206940.2635172614375456No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTA199100.253533810535495No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA193950.24697580388427554No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG193530.24644097615737995No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG190250.2422642262901955No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA188370.23987023551266295No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA187650.23895338798084198No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA181890.23161860772627413No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA179010.22795121759899023No Hit
GGTAAATCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTT177460.22597744860687563No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC177260.22572276873692537No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG174840.22264114231052712No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA171560.21846439244334265No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA170780.2174711409505366No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT168940.21512808614699408No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT168570.21465692838758607No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA164710.20974160689754584No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA163720.20848094154129201No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG161530.20569219696533655No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT161510.2056667289783415No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG158230.20148997911115704No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG156650.19947800813854993No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC155530.19805180086682841No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC153110.19497017444043013No Hit
AATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTATATACAT150410.19153199619610148No Hit
GTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTA146560.18662940869955874No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT145690.18552155126527506No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA145640.18545788129778748No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG145630.18544514730429No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT144980.1846174377269516No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT144760.1843372898700063No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG144390.1838661321105983No Hit
ATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCC143760.18306389052025496No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC143100.18222344694941905No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG142360.18128113143060307No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT142340.18125566344360802No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA141690.18042795386626964No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG140900.17942196837996607No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA139900.1781485690302147No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA137720.17537255844775676No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT137300.17483773072086117No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG136400.17369167130608495No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA136310.17357706536460735No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA136170.17339878945564216No Hit
GACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAG135520.17257107987830375No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT134570.17136135049603998No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA133650.17018982309426875No Hit
AGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTG131160.16701905871338787No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT129050.1643321860854125No Hit
CTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACT128060.16307152072915865No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTATT126620.1612378256655167No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA125960.1603973820946808No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG125050.15923858868640706No Hit
ACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTG124680.15876743092699908No Hit
CCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATT122510.15600415433803863No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT121560.15479442495577483No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC119810.15256597609370998No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA119080.15163639456839148No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT117200.14924240379085893No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT117190.1492296697973614No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA116520.148376492233028No Hit
GAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGT116340.14814728035007277No Hit
TGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAA115030.14647912720189848No Hit
TACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGT114740.1461098413904706No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA112480.1432319588600325No Hit
CTTAAATACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAA112290.14299001298357977No Hit
GTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGG111650.1421750373997389No Hit
ACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTA111020.14137279580939555No Hit
TTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCT110480.14068516016052982No Hit
TGCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC109180.13902974100585305No Hit
AAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA108340.1379600855520619No Hit
GTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGT107900.13739978983817133No Hit
TTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAA107660.137094173994231No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA107660.137094173994231No Hit
GGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTAT107300.1366357502283205No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT105730.1346365132492109No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCT104090.13254813831561865No Hit
AATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGC103500.13179683269926534No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG103070.1312492709788723No Hit
ACCAAAATGGAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC101110.12875340825335962No Hit
GGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCC100310.12773468877355854No Hit
GAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGG98720.12570998380745385No Hit
TGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTG98660.1256335798464688No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT98610.1255699098789812No Hit
AAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTATATACA98080.124895008223613No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC97130.12368527884134921No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT96360.12270476134204067No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTTCCATTGCGAA93410.11894823326027418No Hit
ATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAA91730.1168089223526919No Hit
ATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACT91380.11636323258027892No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA90710.11551005501594551No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT90690.11548458702895048No Hit
GAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTC89180.11356175401082594No Hit
GGGCTACCCCTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGA88650.11288685235545771No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT88640.1128741183619602No Hit
GCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTA87440.11134603914225857No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC86810.11054379755191521No Hit
CTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAAC86090.10962695002009425No Hit
GTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCT84640.10778052096295478No Hit
GAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTACCCC84190.10720749125556665No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA83400.1062015057692631No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT82880.10553933810739238No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA82720.10533559421143217No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT81960.10436781070562115No Hit
GACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCA81250.10346369716729767No Hit
TTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTG79790.1016045341166607No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTT79760.10156633213616816No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC79560.10131165226621788No Hit
CATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAAC79120.1007513565523273No Hit
GCTGTGGAGAGTGATTCACACTCTGGATTTCCCAGGATCCAGCCAGCAAT79120.1007513565523273No Hit
GTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATT79000.10059854863035712No Hit
ATCTACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATG78710.10022926281892924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)