FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_C5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_C5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6372501
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT229150.3595919404328065No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT227620.35719099926386827No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC205360.3222596591197083No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC205120.3218830408971297No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG181780.2852569187513662No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT177700.278854408967531No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC170420.26743032288264845No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG170150.26700662738224756No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA146060.2292035732909261No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT144690.22705371093704024No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC140380.22029027535656723No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT124610.19554331964796867No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC123830.19431931042458842No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA120690.18939188867918577No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG118390.18578263071280804No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG116510.18283245463594278No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA115950.1819536787832595No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA112240.17613178875923283No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT109590.17197329588492807No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA107610.16886619554865506No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA105690.1658532497680267No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC103710.16274614943175372No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG103350.1621812220978859No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG103120.1618202963012481No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT102690.1611455219857949No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT101710.15960766424359918No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT100930.1583836550202189No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG100680.1579913443716996No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA99610.15631225479603691No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT98380.15438208640532186No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT97410.1528599210890669No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT95920.15052174962389178No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT94750.14868573578882138No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG91240.14317769428361016No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA88360.1386582756126676No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG88310.13857981348296378No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA87880.1379050391675105No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC87020.13655549053660407No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG84010.1318320703284315No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA83210.13057667625316968No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA82340.12921143519632244No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG82070.12878773969592158No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC79860.12531971356301083No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC78930.12386031795051895No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT78170.12266769357902024No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA77900.12224399807861937No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG77560.1217104555966331No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA75240.11806981277837382No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT74320.11662610959182274No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA73330.11507255942368624No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA73080.11468024877516693No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA72140.11320516073673428No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG72030.1130325440513858No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG71720.11254607884722183No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT71200.11173007269830167No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT71180.1116986878464201No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA71010.11143191660542698No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC70580.11075714228997374No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG70350.11039621649333597No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA68960.10821496928756857No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT67700.10623772361903121No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC65780.10322477783840285No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA65490.10276969748612044No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT64820.10171830494808867No Hit
CTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACC64380.10102783820669467No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG64260.1008395290954054No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG64170.10069829726193844No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA64090.10057275785441226No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT63780.10008629265024832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)