FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_A11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R1_A11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5214771
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC443870.8511783163632689No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC374930.7189769215177426No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT352130.6752549632572552No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC294230.5642242008325965No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT291920.5597944761140997No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT275330.5279809985903504No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG260690.49990689907572167No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT257320.4934444868240619No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT256380.49164191486069087No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC237680.45578223856809824No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC225960.4333076179184091No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA220010.42189772091622046No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC216400.4149750775249766No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT212400.40730455853190867No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG208230.3993080424816354No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT208090.399039574316878No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA199700.3829506607289179No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA197110.3779839996809064No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA192770.3696614865734277No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA190140.36461812033548546No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC188080.3606678030540555No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT181020.3471293370312905No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA178810.3428913752876205No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG171300.3284899758781354No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT163980.314452926120821No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA163640.31380093200641024No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG163160.3128804697272421No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC161270.3092561495030175No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC159080.30505654035431273No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC157930.3028512661438057No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT155920.2989968303497891No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA153740.29481639749856703No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG151110.28977303126062487No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG138520.26563007272994343No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA136970.26265774662012964No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT136650.26204410510068415No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT131500.2521683118971092No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA131440.2520532541122132No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA130930.251075262940597No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC130310.24988633249667147No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG130230.2497329221168101No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT127330.24417179584683585No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT126270.2421391083136728No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT125000.23970371853337374No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC121130.23228249140758048No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC120630.23132367653344701No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG120000.23011556979203882No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT119520.22919510751287067No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA119070.22833217412615048No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA117720.22574337396599006No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA117630.22557078728864602No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC116980.2243243279522725No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG115290.22108353367770128No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA114080.21876320168229824No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG112970.21663463266172184No Hit
ATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCC111620.21404583250156142No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG111600.2140074799065961No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT109470.20992292854278738No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT107390.20593425866639206No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA107080.2053397934444293No Hit
ACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTG103250.19799527150856674No Hit
TGCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC102240.19605846546281708No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA99470.1907466310601175No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA97900.18773595235533833No Hit
GGTAAATCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTT97630.18721819232330625No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC95060.18228988387026007No Hit
GACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAG92260.17692052057511248No Hit
CAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCAGTTCA91000.1745043070922961No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT89060.17078410538065814No Hit
TACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGT88960.17059234240583143No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA88760.17020881645617805No Hit
GTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGG88350.16942258825938858No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA87280.1673707244287429No Hit
GGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCC87180.1671789614539162No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT86470.16581744433264664No Hit
AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAAT85550.164053224964241No Hit
ACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTA83650.1604097284425337No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT82360.1579359860672693No Hit
TGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAA81450.15619094299634634No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT78490.15051475894147606No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA77240.1481177217561423No Hit
ATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATC76790.14725478836942216No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA74710.14326611849302684No Hit
ATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAAT74000.14190460137175726No Hit
TTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAA73850.1416169569095172No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG73770.14146354652965584No Hit
CATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAAC73500.1409457864976238No Hit
AATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGC71900.1378775789003966No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC71550.13720640848850313No Hit
AAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA70800.13576818617730288No Hit
CTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATT69650.13356291196679584No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT69540.13335197269448648No Hit
CGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAA69460.13319856231462512No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA68820.13197127927573427No Hit
CTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCA68730.13179869259839022No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC68210.13080152512929138No Hit
GTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAA67960.13032211769222465No Hit
CTTAAATACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAA67290.12903730576088576No Hit
CACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT67270.12899895316592042No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT67050.1285770746213017No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA66980.128442840538923No Hit
GAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGG65920.12641015300576No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG65490.12558557221400518No Hit
GACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCA64070.12286253797146604No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA63500.12176948901495388No Hit
GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACACGT61800.11850951844289997No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT61750.11841363695548665No Hit
CGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAA61320.11758905616373182No Hit
TGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTT61190.11733976429645712No Hit
GTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT60940.11686035685939038No Hit
GGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAA60240.1155180160356035No Hit
GTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGT60240.1155180160356035No Hit
GAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAAT59720.11452084856650463No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA59590.11427155669922993No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG59560.11421402780678193No Hit
TTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAG59380.11386885445209387No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA58000.11122252539948543No Hit
ATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAA57510.11028288682283459No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA56870.10905560378394372No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC56110.10759820517526081No Hit
GGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCA55880.1071571503331594No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA55670.10675444808602333No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC55380.10619833545902592No Hit
CGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCT55370.10617915916154323No Hit
TTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAA54960.10539293096475377No Hit
ATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTG54810.10510528650251373No Hit
GAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTC54770.10502858131258305No Hit
TGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTG54700.10489434723020435No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT53560.10270824931717999No Hit
GTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATT52910.10146178998080645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)