FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_Control_1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_Control_1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10069022
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG508110.5046269637706621No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC390030.3873563887336824No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC377800.3752102239919627No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA334420.33212758895551125No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG328590.32633755294208316No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT319840.317647533196372No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA283880.28193403490428365No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA282830.2808912325347983No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA280640.27871624473558604No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC270420.2685663016725954No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA266010.264186531720757No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG259770.25798930621067273No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG256450.25469206443287146No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT233500.23189938407126331No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA231250.22966480756522334No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC227790.2262285254714907No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT224020.22248436839248142No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA218970.21746898556781383No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA214760.21328784463873454No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC214620.21314880432280314No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG214430.212960106751182No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC200270.19889717193983686No Hit
GTCTTTAATGGTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAG192490.19117050295450738No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA191950.1906342045930578No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT189010.18771435795849883No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC188070.18678080155153104No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC186990.18570820482863182No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC186500.185221563722872No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG183680.1824208945019685No Hit
GTATATAGCCCATCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATT183390.1821328824189678No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA182060.18081199941761972No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC181040.17979899140154823No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA180330.1790938583707534No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC180170.1789349551525461No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA179750.17851783420475195No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA179740.178507902753614No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA177070.17585620529977986No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA176840.1756277819236069No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT174800.17360176589146392No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC174780.17358190298918802No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA173310.17212197967190856No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA172510.1713274635808721No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC172470.17128773777632028No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA172070.17089047973080207No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC170520.16935110480441895No Hit
CATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGATA164350.16322339945230033No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG163930.16280627850450619No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT161570.16046245603594866No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA159130.1580391819582875No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA158070.15698644813766421No Hit
CACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGA151660.15062038795823465No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG149560.148534783219264No Hit
GGTCATTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTT149390.14836594854991875No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC149110.148087867918056No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG147270.14626048090867216No Hit
TGTTAACATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGTAATGACG146790.1457837712540503No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC142090.14111598921921115No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC141620.14064921101572725No Hit
CATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATC138560.13761018696751284No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC134980.13405472746012473No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA133600.13268418720308686No Hit
GCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAGT130230.1293372881695958No Hit
TCTCAAAACCGTTTCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA130090.12919824785366443No Hit
GTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGG129670.12878112690587032No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT129440.12855270352969733No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG129110.12822496564214478No Hit
GTTTTTTAACTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT128710.12782770759662657No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA128470.12758935276931563No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT128090.12721195762607332No Hit
AGTTATCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAG127050.12617908670772593No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT126390.12552361093262088No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC125640.1247787520972742No Hit
GCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA125640.1247787520972742No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC125430.12457019162337712No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT125230.124371562600618No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG124300.12344793764478815No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT123860.1230109537947181No Hit
GCTCATTAGGGTTCGATATGGGCTCCTGTCTTTAATGGTTCCATTGGAAT122880.12203767158319845No Hit
GAATAGAAAAGGGAAAGATAGTCAAATCAGTCGAAATGAATGCCCCTAAT122330.1214914417706109No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG121040.12021028457381462No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT120960.12013083296471096No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC119740.11891919592588039No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT118560.11774728469160162No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT117260.11645619604366739No Hit
CAATTCAACTTGGGAATCAAAATCAGATTGAAACATGCAATCAAAGCGTC116950.11614832105839078No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC113980.11319868007041796No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTT113900.11311922846131432No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT113000.11222539785889832No Hit
GGTAAGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT112860.11208635754296693No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA110990.11022917618016922No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT110550.10979219233009918No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT109930.10917644235954593No Hit
TTCCAAGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCC109860.10910692220158025No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA109700.10894801898337296No Hit
GTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACA109420.1086699383515102No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA109260.10851103513330292No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAAC109240.108491172231027No Hit
GAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC108760.10801446257640514No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA108750.10800453112526719No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG108650.10790521661388762No Hit
CTAATGAGCTGTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATT107820.10708090616943731No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG107700.10696172875578185No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA107490.10675316828188477No Hit
TCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTC105280.10455831758039659No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT105260.10453845467812067No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA104770.10405181357236086No Hit
GTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATTTGAGTCAGTC104580.1038631160007397No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT102970.10226415236752884No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG102870.1021648378561493No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA102780.10207545479590768No Hit
GTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCA102470.10176757981063106No Hit
TCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATTTCACGGAAACCA102110.10141004756966465No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC101910.10121141854690555No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA101350.10065525728318005No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG101080.10038710810245523No Hit
CTGCATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA100790.10009909601945452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)