FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_H5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_H5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095772
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC122460.2989912524427629No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG117130.28597783275045585No Hit
TATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGA96580.23580414144146697No Hit
GTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCTTTTAAGGACTTTTG77560.1893660096313955No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA68870.16814900829440702No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT65480.15987218038504097No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG60400.14746914623177265No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT59130.14436838769345559No Hit
ATATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCTTG57590.14060841277297662No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT57310.13992478096925318No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT55470.13543234340192764No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA48720.1189519338478802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)