FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_H2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_H2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG180.5509641873278237No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC150.4591368227731864No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG100.3060912151821243No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG90.27548209366391185No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA90.27548209366391185No Hit
AGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAA80.24487297214569942No Hit
GTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGA80.24487297214569942No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA70.21426385062748698No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC70.21426385062748698No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG70.21426385062748698No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA70.21426385062748698No Hit
GGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTG70.21426385062748698No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT70.21426385062748698No Hit
TCATCATACTGCTCCCATGGAAATATGTAATACCGATACATTTTAACTTG70.21426385062748698No Hit
GTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATG70.21426385062748698No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA70.21426385062748698No Hit
GTATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATT70.21426385062748698No Hit
GGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATC60.18365472910927455No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC60.18365472910927455No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC60.18365472910927455No Hit
CAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGGCTCCCTGCTGAGC60.18365472910927455No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA60.18365472910927455No Hit
GTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCC60.18365472910927455No Hit
ATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGG60.18365472910927455No Hit
CCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTC50.15304560759106214No Hit
TTATTTATGTATGCAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGC50.15304560759106214No Hit
ACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGC50.15304560759106214No Hit
ATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAAT50.15304560759106214No Hit
GTGAAAGATAGCCCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTC50.15304560759106214No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC50.15304560759106214No Hit
TACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGATTATTACCAC50.15304560759106214No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC50.15304560759106214No Hit
GTACTGAAGTGATAAAATTAAAATAAACTAAGAGCCTGGAAATCTAGGAA50.15304560759106214No Hit
AAATTAAACATCTCCCATGTTAAATACTATGGCTTAAAACATTAAGCCAC50.15304560759106214No Hit
GTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAG50.15304560759106214No Hit
ATTATACACAATTCCATCTCAGAATGCATCCTGTTTATTAATTTATTTGT50.15304560759106214No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG50.15304560759106214No Hit
CAATAATATTTAAGCACTTTTGGTATGCCACATACACTTATTTAGTCCTC50.15304560759106214No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT50.15304560759106214No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG50.15304560759106214No Hit
ACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTC50.15304560759106214No Hit
CATAAATAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGA50.15304560759106214No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC40.12243648607284971No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA40.12243648607284971No Hit
ATTAGAAACTGTTTCTTTGTGACTGATGGTGCCTTGAGAAAGCCAGTGGT40.12243648607284971No Hit
GTTAAGCACCTGCCTTCAGCTCAGGGCTTGATCCGGAGTCCCATATCTGG40.12243648607284971No Hit
GTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACA40.12243648607284971No Hit
AGCGAAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGA40.12243648607284971No Hit
AGGTAGAAGTAGGCTCCCTGCTGAGCAAGGAGCCAGATATGGGACTCCGG40.12243648607284971No Hit
ACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGGCTTT40.12243648607284971No Hit
TCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGT40.12243648607284971No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG40.12243648607284971No Hit
GCATTGTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTC40.12243648607284971No Hit
GTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGTTGAT40.12243648607284971No Hit
GCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGT40.12243648607284971No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA40.12243648607284971No Hit
ATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCTCC40.12243648607284971No Hit
TTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGAT40.12243648607284971No Hit
GCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCAC40.12243648607284971No Hit
GCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTA40.12243648607284971No Hit
GATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATT40.12243648607284971No Hit
AGGTAGTTTACTTGTCAGTTGATTTAGTTTACTGCAGAATCTGTAGTTTA40.12243648607284971No Hit
GTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCA40.12243648607284971No Hit
AGTAGGTACTATGGGCTATCTTTCACTGAGTGTTTTGCCAAATATTCTGT40.12243648607284971No Hit
TATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCA40.12243648607284971No Hit
ACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGA40.12243648607284971No Hit
GCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTG40.12243648607284971No Hit
CATTTTAACTTGGCCTAAGGGATTTTTAAAATTATGTACTGAAGTGATAA40.12243648607284971No Hit
ACTATTAGCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAAT40.12243648607284971No Hit
GGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCT40.12243648607284971No Hit
GTCTTTTCATCTGTAAAATGGAAAAAGAACAGTACCTATTTCTTAGACAT40.12243648607284971No Hit
AAGCACAGCAGCAGGGGACATGGGAGCACAATAATGGAGGAGTGGCAGGC40.12243648607284971No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG40.12243648607284971No Hit
AGTTAAATCTGCAAAAACCAGCATTAACCACTGGCTTTCTCAAGGCACCA40.12243648607284971No Hit
GAGGAGGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCAT40.12243648607284971No Hit
GTTTCTAATCTTATGATGCAAATTAAACCAATAAGGAACAAAAGTCACTG40.12243648607284971No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)