FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_H1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_H1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4444
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG260.585058505850585No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA160.36003600360036003No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG150.33753375337533753No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC110.24752475247524752No Hit
AGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAA100.22502250225022502No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA100.22502250225022502No Hit
GGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTG100.22502250225022502No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC100.22502250225022502No Hit
ACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGC90.20252025202520252No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC90.20252025202520252No Hit
CACTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTC80.18001800180018002No Hit
ACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGGCTTT80.18001800180018002No Hit
TACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGATTATTACCAC80.18001800180018002No Hit
GTATTACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATT80.18001800180018002No Hit
CTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGGTGTAAGGAGTAAG80.18001800180018002No Hit
GTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCT70.15751575157515751No Hit
GCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGGGCAGATC70.15751575157515751No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG70.15751575157515751No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA70.15751575157515751No Hit
GTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGT70.15751575157515751No Hit
GTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAA70.15751575157515751No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA70.15751575157515751No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA60.135013501350135No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA60.135013501350135No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT60.135013501350135No Hit
GTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCT60.135013501350135No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG60.135013501350135No Hit
GTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGTTGAT60.135013501350135No Hit
TCCCAGGACCCTGAGCCAAAGGAGATGCTCAACCATCTGAGCCACCCAGG60.135013501350135No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG60.135013501350135No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC60.135013501350135No Hit
GGACATGGGAGCACAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGG60.135013501350135No Hit
CAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGGCTCCCTGCTGAGC60.135013501350135No Hit
GTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAG60.135013501350135No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA60.135013501350135No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG60.135013501350135No Hit
CACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTT60.135013501350135No Hit
TTATTTATGTATGCAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGC60.135013501350135No Hit
CCCTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAA60.135013501350135No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC60.135013501350135No Hit
CTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCC60.135013501350135No Hit
CCTTAAGACTCTTCATCTTTAAAAATTTCATTAAATTTCGGGGCGCCTGG60.135013501350135No Hit
ATTCTTCACAGTGCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTT60.135013501350135No Hit
GGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAATTTGGA60.135013501350135No Hit
GTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATG60.135013501350135No Hit
GTTTTAAGCCATAGTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCA60.135013501350135No Hit
GAACAGTACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTG60.135013501350135No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG50.11251125112511251No Hit
GAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTT50.11251125112511251No Hit
AAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGC50.11251125112511251No Hit
GAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATA50.11251125112511251No Hit
CTTTTGGTATGCCACATACACTTATTTAGTCCTCAAATATGTCTAAGAAA50.11251125112511251No Hit
GTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGA50.11251125112511251No Hit
ATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATTAT50.11251125112511251No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT50.11251125112511251No Hit
ATATCAAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCC50.11251125112511251No Hit
GGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCC50.11251125112511251No Hit
AAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTT50.11251125112511251No Hit
GTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCC50.11251125112511251No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA50.11251125112511251No Hit
CCTTATAGGAAAAGAGTTTAGTAGGGATTCCTAGATTTCCAGGCTCTTAG50.11251125112511251No Hit
GAATGGGGCTAGTGGCTTTTATCCAATGAGGCATACTTGTATCATCATAC50.11251125112511251No Hit
GAGAGAAAGAGAGCAAGAGCGCGAGCAAGCACAGCAGCAGGGGACATGGG50.11251125112511251No Hit
GTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAG50.11251125112511251No Hit
TATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGG50.11251125112511251No Hit
ACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATAAAGGTAGGA50.11251125112511251No Hit
GTACTGAAGTGATAAAATTAAAATAAACTAAGAGCCTGGAAATCTAGGAA50.11251125112511251No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG50.11251125112511251No Hit
CATCAGAATCATCTGGGATCACTTGGAGAAGTAAACTACAGATTCTGCAG50.11251125112511251No Hit
GGTCTGTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAG50.11251125112511251No Hit
ATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAGTTAATATACT50.11251125112511251No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG50.11251125112511251No Hit
ATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACTCAGTG50.11251125112511251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)