FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_G6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_G6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628596
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAG1466122.6047703548096184No Hit
ACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCA929301.6510334015800745No Hit
CCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAA738891.3127430002082223No Hit
TCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATA686501.2196647263367277No Hit
GGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAA619681.1009495085452927No Hit
GTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTA610411.0844800372952685No Hit
CTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTA551240.9793561307295816No Hit
ACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTT526590.9355619056688382No Hit
TAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAA479990.8527703889211449No Hit
ATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTG472050.8386638515182115No Hit
ATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTT453930.8064710986540871No Hit
GTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTA432600.7685753250011194No Hit
ATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGG398170.707405541275302No Hit
ATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAG389790.6925172813966396No Hit
CAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAG379500.6742356353165159No Hit
GGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCT378030.6716239715907839No Hit
CAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTC337790.6001318979013595No Hit
CCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAA296450.5266855180226117No Hit
CTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGA290390.5159190675614309No Hit
ACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTG270630.4808126218332245No Hit
GTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAATCATAG242880.43151080660257013No Hit
TACTAATACAGACCAGGGAAAGAAGGAGGAGGGAGAGGGGATGAGAAGAG218810.3887470338961972No Hit
ATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCATAATCA215610.3830617795272569No Hit
CATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCAC204270.36291465935732464No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACAC202070.35900604697867816No Hit
CTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAG191740.3406533352189427No Hit
GTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGA190050.3376508102553461No Hit
ATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGGAACTGAGTAA188520.3349325480101965No Hit
AAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGG187130.3324630156436881No Hit
AAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCC186710.33171682600776464No Hit
GTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACT179670.3192092663960959No Hit
GTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGC174400.30984636310724734No Hit
TTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACC173080.30750119568005946No Hit
GAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGG169040.30032356203927235No Hit
GTTTTTAAGTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCT163440.2903743668936268No Hit
CATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAA160060.2843693169664335No Hit
ATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCAGT148750.2642754960562101No Hit
CTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTT147350.26178819726979874No Hit
TTATTAACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACT145950.25930089848338733No Hit
GTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCAC145200.25796841699066697No Hit
GATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGAT143010.2540775710319234No Hit
ATATTACGTAGTGTGTATACACACACACACACACACACACACACACACAC138960.2468821709712333No Hit
GGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGT138330.24576288651734818No Hit
CCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAG137940.24506999614113362No Hit
GAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATCCAGTAGGTGG137850.24491009836200714No Hit
TCCTAACACGTAGTACCTCAAATCATAGGTGATTCAGGTGGTTTAGTTAT137650.24455476996394837No Hit
GAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTCTGGGATGATGAA137450.2441994415658896No Hit
ACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCA134010.23808779311927877No Hit
GATTATAAAAGGTCACCAAAAAGGACAAAAAACATTAAAAAGCAATGTCC132360.2351563338352939No Hit
ACTTTATGCTTTAAGAAAGTTTTGGGAAGTTTCTGATAAAATATTGGTTC131130.23297106418723248No Hit
CTTGAGAACTGATACCTTAGCCCCTTGATGATGATTACAGAGAAATAAGG131130.23297106418723248No Hit
GAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAA130680.23217157529160024No Hit
ACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAG126520.22478074461197783No Hit
ATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAA123340.21913102308284338No Hit
AACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAA122060.21685692133526727No Hit
CTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTA121420.21571987046147922No Hit
GGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAA120090.2133569366143884No Hit
GTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAGGTCC118910.21126049906584166No Hit
GTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTAT118670.21083410498817112No Hit
GTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCA116560.2070853903886511No Hit
ATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACC114200.2028925152915576No Hit
TCTTAATATTACGTAGTGTGTATACACACACACACACACACACACACACA111790.19861080809494944No Hit
TGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATT110830.19690523178426733No Hit
GGTTTAACTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGC109620.19475549497601177No Hit
GTATTATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCAT107740.19141540803425935No Hit
TATATAACCAGAACTAAAAATCAGGTATCATTCTTGTCCCATTTACTAAA107650.1912555102551329No Hit
GTATGTAAGTGATCTAAATACAGCTGCCTGAAGGTATCAAGTAAATCCGT106570.18933673690561553No Hit
AGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCA104440.18555248946628963No Hit
ATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACT99020.175923089878897No Hit
TCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCC97400.17304492985462094No Hit
AATATTAACAGCCTGAAAAGGATTCTTGATTACTACCATTCATCAACTAT96450.1713571199638418No Hit
ATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCCTT94200.16735967548568062No Hit
AAAGAGAGGTCCTCCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGG94080.16714647844684535No Hit
ATTCTGGGATGATGAAAATGTTCTAAAAATAAACTGTGATGATAGCTGCA93470.1660627268327661No Hit
CTATAAAACAAAGAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTC93390.1659205954735426No Hit
TCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGGAATTACTGATT91430.16243837717256665No Hit
ATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACC89700.1593647865293583No Hit
CAGTAAATACAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTT89140.15836986701479375No Hit
GAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCAC89040.15819220281576438No Hit
AATGTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGAC88340.15694855342255865No Hit
TCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAA88290.15685972132304396No Hit
CAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAG88060.15645109366527638No Hit
TGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGG87930.1562201302065382No Hit
GTCTTGATCTGAAGTAAATGTACTTCTCTAATGTATCAGCCACTGAGTCA87200.15492318155362367No Hit
TTCTAGGACATTGCTTTTTAATGTTTTTTGTCCTTTTTGGTGACCTTTTA85880.1525780141264358No Hit
ATTAGAAAGAGGTCACTTCTCTGCCATTTCTATTTCTCTGAAGATCCTTA84900.15083690497594782No Hit
ATCATAAGCATTACTATTTGAAAAAAATCCCAGCAGCTGCCTAAAACCTA84700.15048157657788905No Hit
GCATAAAGTACATTAAAAATTTTTTTCTGCGACTTGTCTTGATCTGAAGT84490.15010848175992733No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACACTA84360.14987751830118914No Hit
GAATAGCGATCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATG83710.14872270100749815No Hit
GATTACTACCATTCATCAACTATTCCTCTATCTTCAAGACCTTACTGATG82990.14744351877448658No Hit
GAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTT82620.14678616123807783No Hit
TGATTACAGAGAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATC81190.14424556319195764No Hit
CTGCAAATGAGCACAAAGATCATTCTGGGATGATGAAAATGTTCTAAAAA80430.1428953152793343No Hit
AACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTAC79720.14163389946622568No Hit
GGATGAATATTTGGAGGCAAAGAGAGGTCCTCCCTTATTTCTCTGTAATC79300.14088770983030227No Hit
GAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAAC78740.1398927903157377No Hit
GAATCACCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCT78250.13902223574049372No Hit
CTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTT77810.13824051326476444No Hit
GTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAGGTCCTCCC77090.13696133103175287No Hit
CTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAA75170.13355017841038866No Hit
TATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAG73930.1313471423424243No Hit
CCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACT72880.12948166825261573No Hit
ACCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGA72600.12898420849533349No Hit
GTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCACAGTTTAT72390.12861111367737177No Hit
ATTTAAGGAAATGCTCTGGTCTTCAGCCCATCAGTAAGGTCTTGAAGATA71620.12724309934484548No Hit
ATATAGTACACTGCTACTTGCAAATTTTATAGCAGCTCTGTAAAAGATTT71560.12713650082542788No Hit
GTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAA71310.12669234032785442No Hit
TCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAA71160.12642584402931034No Hit
GATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTT70820.1258217857526104No Hit
ATAGATTACTTGGAAGCTCAAAGAATTGGCTGATTATGATTTATCTCCTC70020.12440047216037535No Hit
AATCATAGGTGATTCAGGTGGTTTAGTTATAAGTAAGGACTATTTGATCA69320.12315682276716966No Hit
AAAGAATAGCGATCTCAGAAGAGTATCAAGAACAATTCAGAACATACAAT68100.12098931953901115No Hit
AGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACT68010.1208294217598847No Hit
ATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATA67470.11987003508512602No Hit
ATTTTAATGATATGAGACCTCATTTGTTTCTAATAGGGAGACAGACAGTT67110.11923044396862023No Hit
GAATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCC66980.11899948050988203No Hit
ATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAGGT66930.11891064841036735No Hit
TTTCTAGGCAATCTAGTTAGTTGTCATCCGTGCAACTAATGGAACCAATA66750.11859085285211445No Hit
ACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGT66700.11850202075259976No Hit
GAATAATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGG66580.11828882371376451No Hit
GTTCTTATACTATAGGAACAAAAACTACTGCCACAGAATGTAATGCTACT66080.11740050271861757No Hit
CTATGAAGAAGGTCTATTATTACCCCAATTTATAGAAAGGCCCAAGCCTT65760.11683197728172355No Hit
ATATGAGACCTCATTTGTTTCTAATAGGGAGACAGACAGTTTAAGCAGGA65660.11665431308269417No Hit
TCCCAGGACCCTGAGATCAGGACCTGAGCCAAAGGCAGATACCTAACCGA64640.11484213825259443No Hit
GTTCTACAGGTCCCTGTGTGATCACTTACTGGCTCAACAGATCTGTTGAG64460.11452234269434154No Hit
CATCTAGAGATTTCTTAATATTACGTAGTGTGTATACACACACACACACA63900.11352742317977699No Hit
GTCTTATTGTGATTTTAATTTGCATTTCCTTTATGACTAATGATGTTTCT63170.11223047452686248No Hit
ACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTC62440.11093352587394796No Hit
ATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAGG61990.11013403697831574No Hit
AAATACAGCTGCCTGAAGGTATCAAGTAAATCCGTGGCACAGAAACCTCA61210.10874825622588652No Hit
GTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAGGTCCTC61180.10869495696617772No Hit
ATTACGTAGTGTGTATACACACACACACACACACACACACACACACACTG60410.10732694263365145No Hit
ATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTT60340.10720257769433088No Hit
GTCTAACTCCTTTTCATGAAACCATTTGAAAAAAGTATGTGGAAAGACAG60070.10672288435695154No Hit
GTAAATGTACTTCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGG59910.10643862163850451No Hit
ATAAAGAGTTGAGGCATGATAATTTAGTAAATGGGACAAGAATGATACCT59780.10620765817976631No Hit
TTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATAC58870.10459091396859892No Hit
GTTTGAGTATGCAGCTATCATCACAGTTTATTTTTAGAACATTTTCATCA58670.10423558557054015No Hit
GGAATAATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGG58100.10322289963607266No Hit
GTATTTACCAGTACACAAGAATAAAGGATTAAAACAAAGAATGGGGGCAC57660.10244117716034336No Hit
GCATTACTATTTGAAAAAAATCCCAGCAGCTGCCTAAAACCTAAACACAA57010.10128635986665235No Hit
AATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGATGATGATTA56820.10094879788849653No Hit
GTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCACAG56770.10085996578898183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)