FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_G6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_G6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208547
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAGGTCACACAAT175990.41817282781919746No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT152280.3618350941548235No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG141750.3368145823249687No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC141230.33557900149386477No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA104900.24925467150539127No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT104740.24887449278812854No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT103950.2469973603716437No Hit
GTCCTAATCTCACCTGGGGGCTATTTCTAGTAGACTCCTGGTGGAGCCTT101870.24205503704722792No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG96550.22941409469824145No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA92170.21900670231317365No Hit
GGTTCACACAGTGTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAG90170.21425446834738926No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG89620.21294760400679852No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT88850.21111799392997155No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT88420.2100962636273279No Hit
GTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAGGTCACACAATTA87540.2080052806823828No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG85300.20268277864070425No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG82920.19702762022142084No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG82130.195150487804936No Hit
ACCTACGCAAGTGCAGAGCTTGGGCTGGAAGTTTCCAAGGGGCTCAAGGA80790.19196649104786045No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC79060.18785580866745696No Hit
GTCTGAGGAGGAGGCCGAGCTGCGGACGGGTGAGGGATGACATCGGGCAG77930.18517079647678877No Hit
GCTCAGGAGAGGGCGCAGGCTGTCTGAGGAGGAGGCCGAGCTGCGGACGG72660.1726486599769469No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC72070.17124675095704053No Hit
GTCCAGGTAGTGAGCTGGCTTTCTCTCTTGGGCGTAACTAGTGGGAAACT70900.16846669408705667No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT69450.16502132446186296No Hit
GCCCTGGAGCCTCTGCGTTCTCAGCTGCCACTCGGGGCTCTTCGGTGCCT68530.16283529683760214No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC68520.16281153566777323No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA66250.15741775011660794No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT63970.15200020339561374No Hit
GCTCTGGCTGGGTTGGAGAGTGGCAATGGAAAAGAACCCCAGGCAAGCCC63180.1501230709791289No Hit
ATCTTATCTTACCTGTCCAGGTAGTGAGCTGGCTTTCTCTCTTGGGCGTA62650.14886372897819605No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA62410.14829346090230192No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA61550.14625000029701463No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG60900.1447055242581347No Hit
GTGCAGAAGGGGCCCTTTGCCAAGGAGCCTGGCTCTGGTTCAAACAGAGG60880.14465800191847686No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG59000.14019090199063952No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT57950.13769597915860274No Hit
CTTCTGCACATCGGCTTCTCTGTCTGCGAACTGAGAAGACAAAAATGACG56940.1352961010058816No Hit
CTTCTGGAAGGAGGTGGAGTCTACAGAATGACTTGCTCACTTGATTGGAG56330.13384666964631736No Hit
GATAAAGCCAGTAGCCCAAGGTCACACAATTAGGCTTTGACATGGTGGGA55760.1324922829660688No Hit
GATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTT55060.13082900107804427No Hit
CCCCAAGGGAGGTTAGATGCTGGGAGCCAGGAATCTCCTCTGTTTGAACC54870.13037753885129477No Hit
GTATAAATTCAGTCTCCCACCCAGAGGAGTTTGGTGGCTTGAGCCTCTAG54420.12930828620899326No Hit
GTTCAGAAGTGAGGCAGCTGCTCCTGCCCTTCTTGGGATAGATCTTGAGC54300.1290231521710462No Hit
GTTTATACCTTGGTTCTATCTTATCTTACCTGTCCAGGTAGTGAGCTGGC54000.12831031707617854No Hit
ATCTTGAGCAGCTCTGGCTGGGTTGGAGAGTGGCAATGGAAAAGAACCCC53240.12650446816918048No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA52700.1252213649984187No Hit
TCTTGGGATAGATCTTGAGCAGCTCTGGCTGGGTTGGAGAGTGGCAATGG52680.12517384265876083No Hit
TTAGAGCACAGTGTCCTAATCTCACCTGGGGGCTATTTCTAGTAGACTCC52330.12434220171474858No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT52240.12412835118628827No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG52070.1237244112991966No Hit
CCCCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGTGCTGCA51890.12329671024227601No Hit
GGTGAGGGATGACATCGGGCAGATTCCAGGCGATCAAGTGTCAGTGGCAC51820.12313038205347357No Hit
TTCCAGAAGAGTATAAATTCAGTCTCCCACCCAGAGGAGTTTGGTGGCTT51760.12298781503450003No Hit
TATCTTACCTGTCCAGGTAGTGAGCTGGCTTTCTCTCTTGGGCGTAACTA51750.12296405386467112No Hit
CTACCTGGACAGGTAAGATAAGATAGAACCAAGGTATAAACAAAAGGCTC51340.12198984590168531No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA51080.12137205548613335No Hit
CCCTTGGGGTGACAGGAAAACAGTGCAAAGGAATCCTAGAGGCTCAAGCC50920.12099187676887059No Hit
CTCCAGGGCTGATAAAAGTTGTAATTAACGTGCACATACAACCCTTTCAT50710.12049289220246323No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC50640.12032656401366078No Hit
CATACAACCCTTTCATCTTCAAAGGCTTTACTCCCATTAGCTAGTTATTC50380.1197087735981088No Hit
GTTTTCCTGTCACCCCAAGGGAGGTTAGATGCTGGGAGCCAGGAATCTCC49720.11814053638939995No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA49450.11749898480401906No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA49360.11728513427555876No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT49190.11688119438846709No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT49020.1164772545013754No Hit
GCCATAAGCACCACATTCCCCCAAAGGGGAATTCTTGAGGATGAACAGAT48770.11588322525565237No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG48500.11524167367027148No Hit
CAATTAGGCTTTGACATGGTGGGAATTAGAGCACAGTGTCCTAATCTCAC48490.11521791250044255No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT48140.11438627155643027No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA48000.11405361517882538No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT47930.11388728699002293No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT47540.11296060136669497No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT47410.11265170615891898No Hit
TCCTGGGACACTCAAGTTTCCCACTAGTTACGCCCAAGAGAGAAAGCCAG46920.1114874088373018No Hit
GTCCCAGGATGGGCTTGCCGGCCTGAGCTCAGAATCCAGCCTACGTGACT46150.10965779876047482No Hit
CTCCTGAGCCCCTCAGGCCACACTTCCTTGAGCCCCTTGGAAACTTCCAG45770.1087548743069758No Hit
GATTAGGACACTGTGCTCTAATTCCCACCATGTCAAAGCCTAATTGTGTG45710.10861230728800225No Hit
AAGCACCACATTCCCCCAAAGGGGAATTCTTGAGGATGAACAGATGTACC45620.10839845675954195No Hit
CTTCTGAACTCTGTCCAGCAGCCTCCGAAGAGGGTGTCGTGGGAACTTCC45600.10835093441988411No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA45420.1079232333629635No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA44650.10609362328613652No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAGAGTACACATCAG44000.1045491472472566No Hit
GCATTAGACAGATAAAGCCAGTAGCCCAAGGTCACACAATTAGGCTTTGA43600.1035987004540997No Hit
AGCCAGAGCTGCTCAAGATCTATCCCAAGAAGGGCAGGAGCAGCTGCCTC43380.10307595471786342No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA43040.10226807494368008No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA42520.10103249411257614No Hit
GCTCTGTGAGCCGCAACCCTGCGGGCTTCCTGCAAGGGGCGGGATTTGAG42200.10027213667805064No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA42160.10017709199873495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)