FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_G2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_G2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4170
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCTGCACAGACTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAAT220.5275779376498801No Hit
GTCTCTATCTGCACAGACTACAGAATGTTTGCTGTCACCAATGCTTCCCT150.3597122302158274No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC100.2398081534772182No Hit
GGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAA90.2158273381294964No Hit
GGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAAA90.2158273381294964No Hit
GAATTAGGGAAGATATTTTAGGGGAAAGAGATACCTGGAATTTATTTACT80.19184652278177458No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA80.19184652278177458No Hit
CTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAATGGACGACTACAAA80.19184652278177458No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT80.19184652278177458No Hit
GTCCCTGGTTAAGCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTC80.19184652278177458No Hit
GTGCCAAGCACCGTGCTTGGTACTTGACGTGTAGTCCCTCGTCCGATTTT70.16786570743405274No Hit
GACTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAATGGACGACTACA70.16786570743405274No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT70.16786570743405274No Hit
GTACTTGACGTGTAGTCCCTCGTCCGATTTTTGTAGTCGTCCATTGAGGG70.16786570743405274No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC70.16786570743405274No Hit
GAGCAGGACCCAGGGGCCAGACCTGAGCCCGGGCAATAAAGGAGAGGAAG60.14388489208633093No Hit
TTTTAGGGGAAAGAGATACCTGGAATTTATTTACTGAAGGTCCAGTTTGT60.14388489208633093No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA60.14388489208633093No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC60.14388489208633093No Hit
GGTTAAGCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTCTCTATC60.14388489208633093No Hit
GTGTAGTCCCTCGTCCGATTTTTGTAGTCGTCCATTGAGGGAAGCATTGG60.14388489208633093No Hit
GCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAAAGAGAT60.14388489208633093No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT60.14388489208633093No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC60.14388489208633093No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA60.14388489208633093No Hit
TTTTAAAGGAATTAGGGAAGATATTTTAGGGGAAAGAGATACCTGGAATT60.14388489208633093No Hit
AGCAGGGATTGTCCCTGGTTAAGCTCACCACTGCTCCCAGCATGCAATCA60.14388489208633093No Hit
TGGTTAAGCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTCTCTAT60.14388489208633093No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT60.14388489208633093No Hit
ATATTTTAGGGGAAAGAGATACCTGGAATTTATTTACTGAAGGTCCAGTT60.14388489208633093No Hit
ATTTTAGGGGAAAGAGATACCTGGAATTTATTTACTGAAGGTCCAGTTTG60.14388489208633093No Hit
TCTGTAGTCTGTGCAGATAGAGACTGAGCACTGATTGCATGCTGGGAGCA50.1199040767386091No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA50.1199040767386091No Hit
AGCGAAAGCAGGTGGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGA50.1199040767386091No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT50.1199040767386091No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG50.1199040767386091No Hit
AGTTTGTGCCAAGCACCGTGCTTGGTACTTGACGTGTAGTCCCTCGTCCG50.1199040767386091No Hit
GCCAAGCACCGTGCTTGGTACTTGACGTGTAGTCCCTCGTCCGATTTTTG50.1199040767386091No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT50.1199040767386091No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA50.1199040767386091No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG50.1199040767386091No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA50.1199040767386091No Hit
GTATATAGCCCATCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATT50.1199040767386091No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG50.1199040767386091No Hit
GCCTAGAGGGCAGGAGAGGGAGCAGGACCCAGGGGCCAGACCTGAGCCCG50.1199040767386091No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC50.1199040767386091No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT50.1199040767386091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)