FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_F1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_F1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4220
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC140.33175355450236965No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA110.26066350710900477No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT110.26066350710900477No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG100.23696682464454977No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC100.23696682464454977No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA100.23696682464454977No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT100.23696682464454977No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT90.21327014218009477No Hit
TTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGT80.18957345971563982No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC80.18957345971563982No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT70.16587677725118483No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA70.16587677725118483No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG70.16587677725118483No Hit
TCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTC70.16587677725118483No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT70.16587677725118483No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT70.16587677725118483No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT60.14218009478672985No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC60.14218009478672985No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT60.14218009478672985No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT60.14218009478672985No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG60.14218009478672985No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC60.14218009478672985No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA60.14218009478672985No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA60.14218009478672985No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA60.14218009478672985No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG60.14218009478672985No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT60.14218009478672985No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC60.14218009478672985No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT60.14218009478672985No Hit
AAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAG50.11848341232227488No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC50.11848341232227488No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA50.11848341232227488No Hit
GTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGTCATCAAGATAC50.11848341232227488No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC50.11848341232227488No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGAC50.11848341232227488No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT50.11848341232227488No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC50.11848341232227488No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG50.11848341232227488No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAG50.11848341232227488No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA50.11848341232227488No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA50.11848341232227488No Hit
GTTTTACTGAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACT50.11848341232227488No Hit
GTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGA50.11848341232227488No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC50.11848341232227488No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT50.11848341232227488No Hit
TCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCT50.11848341232227488No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT50.11848341232227488No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC50.11848341232227488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)