FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19440788
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC1537020.7906161005407806No Hit
GATTAGGAGAGGCCACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTT1152840.5930006540887128No Hit
CCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAGGTGGACCTCTG953910.49067455496145523No Hit
CCATAAGGGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAAT884570.4550072764540202No Hit
ATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGACTCC817200.4203533313567331No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC742920.3821450035873032No Hit
GTCTGTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCA715380.36797891114290227No Hit
CAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGGCTTATCAG692760.35634358031166224No Hit
CCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCACA689600.35471813179589223No Hit
TCCTTGGACAAGCGCCTCAAACTCATCCACCTCTGCCCCACCAACTTTGG669370.34431217500031375No Hit
ACCCAGAGGTGGTGGTACAGGTGGGAATAGTGACTCGGATAACTTGGACA667110.3431496706820732No Hit
CATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGA661870.34045430668756843No Hit
GAGTAGCAGTGACGGGTATCTCCTATCATTAGAGGAATTCACATCCATAG632680.32543948321436356No Hit
GTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACG598950.3080893634558434No Hit
CTCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCT590950.30397430392224845No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG584140.30047135949427567No Hit
CTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAA497250.25577666913501657No Hit
ATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAA490330.25221714263845685No Hit
TCCTTTGGGTGCTCCAGCTCTCGTAACATCACAGGCGACAACATTATCAA474320.2439818797468498No Hit
CCCAAAGGAAATGCATTTTTACTGGAAGCTGCTGTGGTGCCAACTCCAGA467090.24026289469336326No Hit
GTCCAAGGATGGGGGGAAAAATGTTGCCTGTTGAAACTTGGAGTCCCAAC458290.23573632920640872No Hit
ATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGCTGGAAC448710.23080854541492865No Hit
GAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGA445920.2293734184025874No Hit
ACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAG443400.22807717464950494No Hit
GGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGGGTCTCCT441890.22730045716253888No Hit
CCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCAGA436180.22436333342043543No Hit
GTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAA434210.22335000001028762No Hit
GGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAACAGTGA419470.2157680028196388No Hit
GCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTA404150.2078876638128043No Hit
CCCGTAACGTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTAT398630.20504827273462373No Hit
GACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTT396240.20381889869896222No Hit
GCCATAGGTTGATGAAAAATGTTAGAGGGGGCTACCATTCCAGAATCATC376300.1935621128114766No Hit
CCCCTGTGGGGACCAGTTTTGTTGCTCCTGTTGGACACAGTGGGATCTGG374580.19267737501175364No Hit
AACTTGGACAGCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAG367080.1888195066990083No Hit
ACCTATGGCAAGCAGTTTTCTGGATTTTTCTAAAGGTCTGCCAATTTCCA363920.18719405818323825No Hit
ATCCCACTGTGTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTG363240.18684427812288268No Hit
GTACAGAGTTACCCAGGAACGCTGAATTTGCTGGAACGTTCTTGTTAAGT359250.18479189218050215No Hit
GTGCAACATCACTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGG359130.18473016628749822No Hit
GTCGTAGATGATCCTCGTGCCTCTGATTAGGAGAGGCCACCATTGGCTGT352430.18128380392811236No Hit
CCATTAAACAGAGGTCCACCTGGAACATCAGCAAGCTGAGGATGGGAGGG350660.18037334700630447No Hit
GATCCCACTGTGTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTT349760.17991040280877504No Hit
ATCCTGGAAACCACGGAAGCTGGAACGGACAACTTCTTTGACCTTCGTAC347710.17885591880329132No Hit
GTGCTAGGAGTTGTCACCACATTGGCCCTTTTTTTCCCAAACACATTTGT345440.1776882706606337No Hit
CTCCAAGGCACCTGGCTTCAGACCACCTTCCCAGCGAGTTTCTACTAGTC344280.17709158702826241No Hit
CATTGGAACAGAGAGGCTAGCCCGGATTCGGCAAGGAGGATCTGTCGCAC340200.17499290666612896No Hit
GTACCACCCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTT339580.17467398955227537No Hit
TCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCTC336580.17313084222717723No Hit
GAGCAAAAGCAGGGGGGCGTCCGAGCAATGGGTGAGTTCTTGCACTCCAG335450.1725495900680569No Hit
GCTTTAAGTCAGTGCCATTGCCATTAAGTTCTCCAAACATAAAGCTGAAT334940.17228725502279024No Hit
GTTCCAAGCAGGATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTG334650.17213808411469741No Hit
CTGCTACTCTCACTGTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACT332980.17127906543705945No Hit
GTTGGACACAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGG328540.1689952073959142No Hit
CCTCTAATGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAA326340.16786356602417557No Hit
CTTTAAGTCAGTGCCATTGCCATTAAGTTCTCCAAACATAAAGCTGAATC318340.16374850649058054No Hit
ATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATTCTC316620.16286376869085759No Hit
ACTCTGTACTTGGACACTTGGAAAATGTGCACCCCGACAACTCCAAGGCA315020.1620407567841386No Hit
AATGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAAAAGAC314430.16173727114353595No Hit
TTTTCCAAGTGTCCAAGTACAGAGTTACCCAGGAACGCTGAATTTGCTGG313410.16121260105300259No Hit
ATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCA312840.16091940306123395No Hit
GTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTGTGCGACAGAT310490.1597106043232404No Hit
CCTCAAACTCATCCACCTCTGCCCCACCAACTTTGGGCCAACCAAAAGGA306150.15747818452626508No Hit
CCTCTGGGTTCTCCATTTATTTTATTTTTATCAGCTTTTTGCTGTGCTAA306020.15741131480884418No Hit
GTTCCAATGGGGGGAGGTATCTTTCGATCCTGACTGGCACTGCCTCCTTT304490.1566243096730441No Hit
GTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACTATTCCCACCTGTAC302840.15577557864424016No Hit
ATCTTGAATAAGTGCATTGATAAGTTGAACTGCATATCTTGTTGATTCGG300310.1544741910667407No Hit
ATGTTAGAGGGGGCTACCATTCCAGAATCATCTCTCTCCATTGGTTGATG297760.1531625158404073No Hit
TCTCTTTTCTGTCCCCTTTTGGGGCTAGTTAGATTCAGCTTTATGTTTGG297310.15293104374164257No Hit
GGTTAGCTGAGGTGATGGGATTTCCCCTTTGAGAGCAGGGACTGAAGGAA296470.1524989624906151No Hit
GCCTTGGAGTTGTCGGGGTGCACATTTTCCAAGTGTCCAAGTACAGAGTT296420.15247324336853013No Hit
TTCCTGGGTAACTCTGTACTTGGACACTTGGAAAATGTGCACCCCGACAA296240.15238065452902425No Hit
GTTCCCTATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGT294730.15160393704205816No Hit
ACTTTGGGCCAACCAAAAGGAGGCAGTGCCAGTCAGGATCGAAAGATACC294520.1514959167293013No Hit
CCCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCAC292630.15052373391448948No Hit
GTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATT287270.14776664402698078No Hit
ATTCAAGATCCTGCTAAGGAACTGGAAGACTTGATTCCTAAAAATCATAT286840.14754545957705006No Hit
GTGTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTGTGCGACAG285970.1470979468527716No Hit
ACCCAGGAACGCTGAATTTGCTGGAACGTTCTTGTTAAGTTTATTAGTTG285680.14694877594467878No Hit
CATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCTTTTACCA285390.14679960503658598No Hit
ACCAAAAGGAGGCAGTGCCAGTCAGGATCGAAAGATACCTCCCCCCATTG284800.14649611939598334No Hit
TTCCAGGATAATGGGAAGGGGAGGGTGCAACATCACTGCTATACAAGACG283590.14587371664152707No Hit
CCTTTAGAAAAATCCAGAAAACTGCTTGCCATAGGTTGATGAAAAATGTT282960.14554965570325648No Hit
TTCCAGAATCATCTCTCTCCATTGGTTGATGCTGGGAGAATGTGCTTGGG279450.14374417333289166No Hit
ATTCCCACCTGTACCACCACCTCTGGGTTCTCCATTTATTTTATTTTTAT277480.14273083992274388No Hit
TGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAAAAGACAA276940.14245307340422622No Hit
ACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTTAGCTGAGGTGATGG276290.14211872481712162No Hit
CCCTATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGA274270.14107967228488885No Hit
GGGTTGTGAAGTCCATTAAACAGAGGTCCACCTGGAACATCAGCAAGCTG273550.1407093169268653No Hit
CTGTTGGACACAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAA273180.14051899542343654No Hit
CCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCCAGAAAACTGCT273020.14043669423276464No Hit
CTTGGAAAATGTGCACCCCGACAACTCCAAGGCACCTGGCTTCAGACCAC269010.13837402064155013No Hit
GCATTGGATGGTTCCCTATCACAGACTGTGACCAACCTGATAAGCCTTCT265990.136820585667618No Hit
ATCCAATGCATCAACAATTATCAGACCCAAGCACATTCTCCCAGCATCAA264630.13612102554690686No Hit
GAACAACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAA263860.13572495106679833No Hit
TCTTTGACCTTCGTACAACTTCTTTCCACCCTTCTTCTCTTTTCTGTCCC260520.13400691371152237No Hit
CCACAGGGGCTTGTGCGACAGATCCTCCTTGCCGAATCCGGGCTAGCCTC259990.13373429101742174No Hit
TCCTAGTGCAACAACCACCACCACAATCGGAATTTCTGCAACATCTGCAA259820.13364684600233281No Hit
CATTTAATATTACCAGAACAACATATATCCTTAGCACAGCAAAAAGCTGA258000.13271066995843994No Hit
GATTTGGTGCTGGCAGGTGTTCTGATATGATTTTTAGGAATCAAGTCTTC256920.13215513692140463No Hit
GCTATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGA255270.13130640589260065No Hit
GTGCTAAGGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGG255090.13121381705309476No Hit
TTCTAATTCCTTATCAACAAGCTTTAAGTCAGTGCCATTGCCATTAAGTT254840.13108522144266993No Hit
GTACTTGGACACTTGGAAAATGTGCACCCCGACAACTCCAAGGCACCTGG253760.1305296884056346No Hit
CTCCCAGCATCAACCAATGGAGAGAGATGATTCTGGAATGGTAGCCCCCT252830.13005131273485415No Hit
CGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAAGAACGGCG250580.12889395224103056No Hit
GTTTATTAGTTGGCTGGAACGTTGATAATGTTGTCGCCTGTGATGTTACG250380.1287910757526907No Hit
GTACTGCTACTTCCAAAACTCAAACACGACTTGAAGGTGAAACGAATTCT248080.12760799613678211No Hit
GTCAGAAGGCTTATCAGGTTGGTCACAGTCTGTGATAGGGAACCATCCAA247250.1271810587101716No Hit
AAACAAAGGAGACCCCTCCCACTGCACATTTAATATTACCAGAACAACAT246890.12699588103115986No Hit
CTCCTATCATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCT244730.12588481495708917No Hit
CCTCTGCCCCACCAACTTTGGGCCAACCAAAAGGAGGCAGTGCCAGTCAG244200.12561219226298853No Hit
CCTATCATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCTTT243990.12550417195023164No Hit
CTAATAAACTTAACAAGAACGTTCCAGCAAATTCAGCGTTCCTGGGTAAC242180.12457313973075576No Hit
GGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAATGCACTTA241180.1240587572890564No Hit
GTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGAATTCCTCT238910.1228911091463988No Hit
CCCCTCCCACTGCACATTTAATATTACCAGAACAACATATATCCTTAGCA238890.1228808214975648No Hit
GCAGGGGGGCGTCCGAGCAATGGGTGAGTTCTTGCACTCCAGCAGGAACT238230.12254132908604322No Hit
GTATGGAGGCACCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAG235980.12138396859221962No Hit
GTCCAAGTTATCCGAGTCACTATTCCCACCTGTACCACCACCTCTGGGTT234430.12058667580758559No Hit
GGTCAAAGAAGTTGTCCGTTCCAGCTTCCGTGGTTTCCAGGATAATGGGA233440.1200774371903032No Hit
GTACAGGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAAC231550.11910525437549137No Hit
GTGCCAGTCAGGATCGAAAGATACCTCCCCCCATTGGAACAGAGAGGCTA228640.11760840147014616No Hit
AGTCTGTGCTGTCCAAGTTATCCGAGTCACTATTCCCACCTGTACCACCA227940.1172483337609566No Hit
GTTTAATGGACTTCACAACCCAGATCCTGCTTGGAACCCTATGATAAAAG227490.11701686166219187No Hit
CTCTAACATTTTTCATCAACCTATGGCAAGCAGTTTTCTGGATTTTTCTA226050.11627615094614478No Hit
GGCCAGCTCCTGTTGGGACTCCAAGTTTCAACAGGCAACATTTTTCCCCC225200.1158389258707003No Hit
GAAAAGAGAAGAAGGGTGGAAAGAAGTTGTACGAAGGTCAAAGAAGTTGT224450.11545313903942576No Hit
CCATAGAACCTCCTAGTGCAACAACCACCACCACAATCGGAATTTCTGCA222720.11456325741528585No Hit
CATCAATATGCGCACCCGTAACGTCTTGTATAGCAGTGATGTTGCACCCT219600.11295838419718376No Hit
GAATCTAACTAGCCCCAAAAGGGGACAGAAAAGAGAAGAAGGGTGGAAAG219170.11273719974725305No Hit
GCATATCTTGTTGATTCGGTACCACCCCTTATGGTGATCATTCTCTCGCC217670.11196562608470398No Hit
CCATTGATCCATCAGGCAGCTCCCCATCTTCCTCTTCTGCTCCTCTGACA215870.11103973768964509No Hit
GAATTAGAATTCGTTTCACCTTCAAGTCGTGTTTGAGTTTTGGAAGTAGC213720.10993381543999144No Hit
GCCATTAAGTTCTCCAAACATAAAGCTGAATCTAACTAGCCCCAAAAGGG213580.1098618018981535No Hit
TTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCA213580.1098618018981535No Hit
CCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTTAGCTGAGGTGATGGG213390.10976406923423063No Hit
GATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTGGAACATCAGCA212240.10917252942627634No Hit
GCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGC210490.10827236015330242No Hit
GAAGAGGAAGATGGGGAGCTGCCTGATGGATCAATGGATGGTAAGCCAAC209630.10782999125344096No Hit
CCATTGGTTGATGCTGGGAGAATGTGCTTGGGTCTGATAATTGTTGATGC206560.10625083715742387No Hit
ATTCTGGAATGGTAGCCCCCTCTAACATTTTTCATCAACCTATGGCAAGC205710.1058136120819794No Hit
CTGTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACA205220.1055615646855467No Hit
GGATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTGGAACATCAGC204690.10528894199144602No Hit
AGTTTGAGGCGCTTGTCCAAGGATGGGGGGAAAAATGTTGCCTGTTGAAA204200.10503689459501332No Hit
GACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGACTCCAAATGA203760.1048105663206656No Hit
AAGCAGGGGGGCGTCCGAGCAATGGGTGAGTTCTTGCACTCCAGCAGGAA203020.10442992331380807No Hit
TACCAGAACAACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAA200350.10305652219447071No Hit
CAGTAGAATTTTGAATAACTTTTATCATAGGGTTCCAAGCAGGATCTGGG199620.10268102301203016No Hit
ACAATTATCAGACCCAAGCACATTCTCCCAGCATCAACCAATGGAGAGAG198700.10220779116566674No Hit
TATCAGACCCAAGCACATTCTCCCAGCATCAACCAATGGAGAGAGATGAT195160.10038687732205093No Hit
GACTGGCACTGCCTCCTTTTGGTTGGCCCAAAGTTGGTGGGGCAGAGGTG194850.10022741876512414No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)