FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4349317
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG363900.8366830930005792No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC311630.7165033038520761No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA290320.6675071051385769No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC287860.6618510446582763No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC276840.636513733075791No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG258840.5951279246833469No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG209080.4807191565940123No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAA192320.44218437055749216No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA190720.43850563203371934No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC176420.4056269064774998No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC165100.37959983142180714No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT161180.37058692203856375No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA159740.36727605736716823No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA142700.32809749208898775No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC128360.2951267980696739No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT122910.2825960949730728No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA122060.2806417651323185No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG119970.27583641293564026No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG119230.27413499636839533No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT110450.253947918719192No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG109230.25114288059481527No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA108580.24964839306953254No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA107310.24672839436628785No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA104070.23927894885564788No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA101450.23325501452296996No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA101390.23311706182832845No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG100460.23097879506138552No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA100070.23008210254621586No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA99930.2297602129253858No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA97930.22516178977066972No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG96700.22233375953051937No Hit
TACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGAC94870.21812620234395422No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC92850.21348179495769104No Hit
GGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAGGATATGCT88740.20403203537474965No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTT82810.19039771072101666No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC81480.1873397593231305No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAT79820.18352306810471622No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG78070.1794994478443397No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA77170.1774301574247175No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA76790.17655645702532144No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT74460.17119929405007728No Hit
GTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT73480.16894606670426643No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC72750.16726764225279508No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG72430.16653189454804054No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG71010.16326701410819217No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATATCCTG70530.16216339255106033No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70310.16165756600404158No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA69400.15956528346864576No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT66880.1537712702937036No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG66830.15365630971483568No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA65220.1499545790752893No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA63790.14666670651966734No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGACCCCTCACTT63030.14491930572087525No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA62720.14420655013189426No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG61420.14121757508132887No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC60820.13983804813491404No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC60780.13974607967181973No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT60740.13965411120872542No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC60010.13797568675725408No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC59950.1378377340626126No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT59900.1377227734837447No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA59760.13740088386291457No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC59710.13728592328404668No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT57920.13317033456057584No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA57790.1328714370555193No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT56610.13015836739423686No Hit
AGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTG56330.1295145881525766No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA56330.1295145881525766No Hit
ATCTTGATGACCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT55030.1265256131020112No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAACCCTTTTACTCCATTTGC54970.1263876604073697No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA54960.12636466829159612No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG54620.1255829363552944No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC54620.1255829363552944No Hit
CTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGAAGCAAGGTCTTATAC54240.12470923595589838No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA53990.12413443306155886No Hit
GGCTCGAATCGACCGTGGGTGTCTTTCAACCAGAATCTGGAATATCAGAT53870.1238585276722759No Hit
ATCCACGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAAT53800.12369758286186083No Hit
GTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGG53410.12280089034669123No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA53180.12227207168389888No Hit
GGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTG53110.12211112687348381No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC52290.12022577338005025No Hit
GAATAAATGAGTGGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAA52230.12008782068540877No Hit
CAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCC52200.12001884433808802No Hit
GTCTGAACTAGGTGTTTCCACAATGTAGGACCATGAGCTTGCTGTGGAGA52020.11960498625416358No Hit
CCTCATAATCGATGAAATCTCCTGGGTAACACGTTCCATTGTCTGAACTA52010.11958199413838999No Hit
GATTAGTTCAACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTT51730.11893821489672976No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG51720.11891522278095618No Hit
GTCCACGATTGCAATACAACTTGTCAAACACCCAAGGGTGCTATAAACAC51600.11863931739167322No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA51590.11861632527589966No Hit
CTCTTAGCTCCTCATAATCGATGAAATCTCCTGGGTAACACGTTCCATTG51210.1177426248765036No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT50680.11652404274050386No Hit
GGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTTGTCA50650.11645506639318311No Hit
GTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTAT50360.11578829503574928No Hit
GTTTATAGCACCCTTGGGTGTTTGACAAGTTGTATTGCAATCGTGGACTG50320.11569632657265497No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT50310.11567333445688138No Hit
GCTATAAACACCAGCCTCCCATTTCAGAATATACATCCGATCACAATTGG49970.11489160252057967No Hit
GTAATAGTTCATTCTCCCTTCTTGACCCCTCACTTTGGGTCTTATTGCTA49760.11440876808933448No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG49510.11383396519499499No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA49250.1132361701848819No Hit
CTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTT49210.11314420172178759No Hit
GAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC48850.1123164855539387No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT48690.11194861170156142No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA48450.1113968009229955No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA48310.11107491130216537No Hit
GTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCA48100.11059207687092021No Hit
AATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCTGGCCAAGACCA47900.11013223455544859No Hit
GTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTCTAGA47510.10923554204027898No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC47430.10905160511409033No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA46610.10716625162065677No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA45870.10546483505341182No Hit
AAATAGGCCTCTAGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCA45690.10505097696948738No Hit
AATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGG45440.10447617407514789No Hit
CTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGATGGTGATAAC45290.10413129233854418No Hit
GGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA45280.10410830022277061No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC44880.1031886155918274No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC44630.1026138126974879No Hit
AGTCCAGATTGTGTTCTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGA44610.10256782846594076No Hit
CACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTA44580.10249885211862No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG44560.10245286788707285No Hit
GGGTAACACGTTCCATTGTCTGAACTAGGTGTTTCCACAATGTAGGACCA44530.10238389153975211No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA44240.10171712018231828No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT44200.10162515171922397No Hit
ACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTG44050.10128026998262026No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT43880.1008894040144694No Hit
CCTCTAGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCAGTCTCAA43640.10033759323590348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)