FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC90.2517482517482518No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC80.22377622377622378No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC80.22377622377622378No Hit
GTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGC70.1958041958041958No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG70.1958041958041958No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG70.1958041958041958No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC70.1958041958041958No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG70.1958041958041958No Hit
CCATAAGGGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAAT60.16783216783216784No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG60.16783216783216784No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA60.16783216783216784No Hit
GGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGAC60.16783216783216784No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG60.16783216783216784No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC60.16783216783216784No Hit
CCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAGGTGGACCTCTG60.16783216783216784No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC60.16783216783216784No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG60.16783216783216784No Hit
ATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGT60.16783216783216784No Hit
AGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCT60.16783216783216784No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA60.16783216783216784No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT60.16783216783216784No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC50.13986013986013987No Hit
CTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCA50.13986013986013987No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG50.13986013986013987No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA50.13986013986013987No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA50.13986013986013987No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA50.13986013986013987No Hit
ATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCTCAGCAGG50.13986013986013987No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA50.13986013986013987No Hit
GCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTC50.13986013986013987No Hit
GTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATC50.13986013986013987No Hit
TGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAAAAGACAA50.13986013986013987No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG50.13986013986013987No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA50.13986013986013987No Hit
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC50.13986013986013987No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG40.11188811188811189No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA40.11188811188811189No Hit
GAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCG40.11188811188811189No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG40.11188811188811189No Hit
GGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATC40.11188811188811189No Hit
GTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAA40.11188811188811189No Hit
GTGTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTGTGCGACAG40.11188811188811189No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA40.11188811188811189No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA40.11188811188811189No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA40.11188811188811189No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA40.11188811188811189No Hit
GAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGG40.11188811188811189No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA40.11188811188811189No Hit
GAATAAATGAGTGGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAA40.11188811188811189No Hit
GCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATG40.11188811188811189No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT40.11188811188811189No Hit
ACGTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCA40.11188811188811189No Hit
GATTAGGAGAGGCCACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTT40.11188811188811189No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG40.11188811188811189No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG40.11188811188811189No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT40.11188811188811189No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA40.11188811188811189No Hit
CTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAA40.11188811188811189No Hit
GGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAGGATATGCT40.11188811188811189No Hit
GGGTAACACGTTCCATTGTCTGAACTAGGTGTTTCCACAATGTAGGACCA40.11188811188811189No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG40.11188811188811189No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC40.11188811188811189No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT40.11188811188811189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)