FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_E1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5313
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC170.3199698851872765No Hit
ATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGT150.282326369282891No Hit
GATTAGGAGAGGCCACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTT130.24468285337850554No Hit
CTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCA130.24468285337850554No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT120.2258610954263128No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC110.2070393374741201No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC110.2070393374741201No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA110.2070393374741201No Hit
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC100.18821757952192736No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA90.16939582156973462No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG90.16939582156973462No Hit
GAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCG80.1505740636175419No Hit
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT80.1505740636175419No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA80.1505740636175419No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA80.1505740636175419No Hit
GCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTC80.1505740636175419No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC80.1505740636175419No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT70.13175230566534915No Hit
ATTTATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAG70.13175230566534915No Hit
GAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGG70.13175230566534915No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC70.13175230566534915No Hit
CATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGA70.13175230566534915No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC70.13175230566534915No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG70.13175230566534915No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA70.13175230566534915No Hit
ATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGACTCC70.13175230566534915No Hit
GTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACC70.13175230566534915No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT70.13175230566534915No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA60.1129305477131564No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT60.1129305477131564No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA60.1129305477131564No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG60.1129305477131564No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC60.1129305477131564No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG60.1129305477131564No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG60.1129305477131564No Hit
GCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGTGCCCCTCATTTC60.1129305477131564No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC60.1129305477131564No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT60.1129305477131564No Hit
CACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAG60.1129305477131564No Hit
TTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAA60.1129305477131564No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG60.1129305477131564No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT60.1129305477131564No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG60.1129305477131564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)