FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_D2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_D2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334618
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGATAGATAGATCTTTACTCTGGACAGTTAGGTGGGAACAGTTTT141850.32724913706351977No Hit
GTGCAGGGGTTGATCCCCCGGACCCTGGGATCATGACCTGAGCCGAAAAG128400.2962198745079728No Hit
ATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTCTT113390.26159167889765605No Hit
ACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTCCTGAGCC109970.25370171027758387No Hit
GTGTGAGGCGTGACTGATTTGCAATTCAAAAAGCTCCAACAAAGGCAGAA104520.2411285146695741No Hit
CTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTC99000.22839382847577339No Hit
TGGTATTACTGTCCAACTGAAAGGAAACATACTCTTAGAAGTGTATTTTG93720.21621282429039884No Hit
ATATTAATATGGCTACTCAAAGCAACTCACTAGTCAGCTAGACATTAGAT92270.21286766215615768No Hit
ATATTGTACCTTGTCATTTGCAAATAGTGACAGTTTTACTTTTTCCTACC90610.2090380282645437No Hit
GTCAATGACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTC89710.2069617207329458No Hit
ATTCCATAGTGTCTGAGAATGAGGGCCATACCCTGCTCCCTTAACCCAAA85790.19791824792865254No Hit
CTATATTACCTGGCAAACAATATTTCGTGTTTCTAACTTATCTTTTCCTT80560.18585259416170008No Hit
GGCTTAAAGATATGAAATGGAGTTATTTAATCCTCCCTCCTCAAAGTGCA78440.1809617364206027No Hit
CTTCAGGACCCCCCGGTTGGGGGGAACGTGCCCAGTAAAGAACTGAAATG74640.1721951046205225No Hit
TTCCTGAGCCCAGCAGGTGTCCTGGCTCTATTCTCTACGCATTGGGAAAT74120.1709954602689326No Hit
TATAAAAACCACTCAAGCTTTAGGTACTGCTATTAATGCTAGAAATAACT69570.16049857219252078No Hit
GTATACAATGGACAGAATAGTTAATGTCAAAGTATTCTCAGGAAAGGAAA65710.15159351989033407No Hit
GTGTAAGGGCTCTTTATTTCTCATTGCGATGATGTACTCGTTAGGAATAA65450.1509936977145391No Hit
CATGTAAATAATGGCTAATTTCAAATACACCTGACCCTTTTGGAAATGTT64180.14806379708661754No Hit
CTTTAAGCCTGTGTCTCATTGAGCAAAAGAAGAGTCTACTTTCATAGCAC62790.14485705545448294No Hit
GTATAGGAATAAAGCACTCTCATTAATCAAATATTTTATCTTTTGCTTTC61300.14141961298550415No Hit
GGCTATTAGTTGTCCAAATGGAATAATCCCCTTAAGCCTTTTCAAGAAAA61090.14093514122813128No Hit
AATGTATACAATGGACAGAATAGTTAATGTCAAAGTATTCTCAGGAAAGG60650.13992005754601675No Hit
GCACTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAA59930.13825901152073838No Hit
ATCCAGAACATTCTGCAAGTTTGTCCTGATCTAAAATAACAACAATGCTG59490.13724392783862382No Hit
CAATTTATACTGTATAGTTTGCAGCCTTTTAGTTACAAAAGGTCTGTTTT58120.13408332637385809No Hit
CTATGGAATCTTGTAGACAAAGAGAAAATTTTTTTAAGCTTGCAGCTTGG56100.12942316946960492No Hit
TACTTAGAAAGTGCTATGAAAGTAGACTCTTCTTTTGCTCAATGAGACAC55730.12856957637328134No Hit
CCCAAAAGGTGTGCCACAGGGAGGATCGGGGTGGATAGTCTGGCATCAAC55330.12764677302590446No Hit
TCTTCATACTTAGAAAGTGCTATGAAAGTAGACTCTTCTTTTGCTCAATG54870.1265855491764211No Hit
GTATAAGCCATTAGAAATGAAATTACAGAGCTATAGGAAGGGAACAAGGT54730.12626256800483918No Hit
ACTTAATACTTAAAACTCTAAATCTTGAGATGGTAGAATAGCTAACCAAA54390.12547818515956885No Hit
GTATGATGGCTTATTGATGAATAAACTTTGTAACATTTTCATTCTTGTCA54120.12485529290008947No Hit
GAATAGTTAATGTCAAAGTATTCTCAGGAAAGGAAAGAAAGAGAAAAGCT53820.12416319038955682No Hit
GTTCTATGCTCTCCAGGAAAGCAGTGGGATCTAATAAAATGTTGGTCCTC52560.1212563598453197No Hit
TGGATGGACTGAGCATTCCTGAGCCCAGCAGGTGTCCTGGCTCTATTCTC52150.12031048641425843No Hit
GTAGAATAGCTAACCAAAATTCTGACCTCCATAGTGCTTTCTTGTAAAAT51730.11934154289951271No Hit
CACTAAAGCTGTCTGAATTCATGTATCTTCATGTATATCCATGGAACACA50310.11606559101632485No Hit
CCTTATAACAGTACAATGTTACTGCCACACACATCAGTTTTCTTGCCTAA50240.11590410043053391No Hit
CCATAGTGCTTTCTTGTAAAATCTGAGAAAGAATATCTAAGAATCCATTT50120.11562725942632084No Hit
GTCTGAGAATGAGGGCCATACCCTGCTCCCTTAACCCAAAGATTGGCTTT49560.11433533473999323No Hit
GTCTACAAGATTCCATAGTGTCTGAGAATGAGGGCCATACCCTGCTCCCT48730.11242051779418626No Hit
GGATTAAATAACTCCATTTCATATCTTTAAGCCTGTGTCTCATTGAGCAA48340.1115207845304938No Hit
GTATAGTGCCTGCTGGAGACCCAAAACCTGTTCTGGGGAAGGCCATGATG48040.11082868201996116No Hit
CCTTACAGGCATATTTAGCCTCTAATGCACCTGAACTATGAAGCCTCAGA46790.10794492155940846No Hit
ACCCAGGTGCTCCCAAGCTGCAAGCTTAAAAAAATTTTCTCTTTGTCTAC46430.10711439854676928No Hit
TTCCTATACTAGGTAGAAATAGACATTAATTGGATCCTTTCTCTGAAGAA45980.10607624478097032No Hit
AACGTGAACAGTGTGAGGCGTGACTGATTTGCAATTCAAAAAGCTCCAAC44920.10363081591042163No Hit
GGTTAAAGGCAGTGCACCCATGTAAATAATGGCTAATTTCAAATACACCT44780.10330783473883973No Hit
ATTCTCTACGCATTGGGAAATGCGAAATAGGCCCGCAGAGCCACTTCAGG44570.10282336298146688No Hit
GTAGTGTACTGTGCATTCCTGGGGTCATATTTTAAATGCTAGTCATACTA44340.10229275105672518No Hit
CAATATATCTGCACCATATTTTTGAACCGTTTTTTTATTATAACTCTTAC44290.10217740063830308No Hit
ATTTAATCCTCCCTCCTCAAAGTGCAAAGAGTTCGAGAACTAGAATTGCA44210.10199283996882771No Hit
GTTCAAGCCTCCATAAAAGCAATGCGGCGGGTGGAAGTGGTTTTCTTCAT43940.10136994770934833No Hit
GGCTTGAACTACTCCACAATGATGACCCCTATGCCGGGATATCAAGGGTC43890.10125459729092622No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)