FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_D2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_D2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG60.2285714285714286No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG60.2285714285714286No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC60.2285714285714286No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA50.19047619047619047No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC50.19047619047619047No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT50.19047619047619047No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA50.19047619047619047No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC50.19047619047619047No Hit
CAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCAGTTCA50.19047619047619047No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC40.1523809523809524No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG40.1523809523809524No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC40.1523809523809524No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT40.1523809523809524No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA40.1523809523809524No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC40.1523809523809524No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG40.1523809523809524No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC40.1523809523809524No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA40.1523809523809524No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT40.1523809523809524No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA40.1523809523809524No Hit
GAACAACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAA40.1523809523809524No Hit
ATTCATATCATGCTCCCCCTTGGAATGCAGAACCTTCTTCTTGACTCAAG40.1523809523809524No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.1523809523809524No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT40.1523809523809524No Hit
GAGCTCAATTGCTCTCTTAGCTCCTCATAATCGATGAAATCTCCTGGGTA40.1523809523809524No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT40.1523809523809524No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40.1523809523809524No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC40.1523809523809524No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG40.1523809523809524No Hit
CGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAA40.1523809523809524No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT40.1523809523809524No Hit
GAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGG30.1142857142857143No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG30.1142857142857143No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG30.1142857142857143No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT30.1142857142857143No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA30.1142857142857143No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA30.1142857142857143No Hit
CAATTGGAATGGCTAACTTAATATTACAAATTGGAAACATAATCTCAATA30.1142857142857143No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA30.1142857142857143No Hit
CAATAGGACAGCTCATTAGGGTTCGATATGGGCTCCTGTCTTTAATGGTT30.1142857142857143No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA30.1142857142857143No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA30.1142857142857143No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA30.1142857142857143No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT30.1142857142857143No Hit
GTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCA30.1142857142857143No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.1142857142857143No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT30.1142857142857143No Hit
CATTCATATCATGCTCCCCCTTGGAATGCAGAACCTTCTTCTTGACTCAA30.1142857142857143No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA30.1142857142857143No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA30.1142857142857143No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT30.1142857142857143No Hit
GCTATAAACACCAGCCTCCCATTTCAGAATATACATCCGATCACAATTGG30.1142857142857143No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG30.1142857142857143No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA30.1142857142857143No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG30.1142857142857143No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT30.1142857142857143No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC30.1142857142857143No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT30.1142857142857143No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA30.1142857142857143No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG30.1142857142857143No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA30.1142857142857143No Hit
ATTTGCAGACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCC30.1142857142857143No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT30.1142857142857143No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT30.1142857142857143No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC30.1142857142857143No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG30.1142857142857143No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT30.1142857142857143No Hit
GATAGGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATG30.1142857142857143No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.1142857142857143No Hit
GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGA30.1142857142857143No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTC30.1142857142857143No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT30.1142857142857143No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.1142857142857143No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.1142857142857143No Hit
CAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG30.1142857142857143No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT30.1142857142857143No Hit
CCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCC30.1142857142857143No Hit
CTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATT30.1142857142857143No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA30.1142857142857143No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC30.1142857142857143No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT30.1142857142857143No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG30.1142857142857143No Hit
TTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC30.1142857142857143No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30.1142857142857143No Hit
GTGTATGGGCTTGCAGTAGCAAGTGGGCATGACTTTGAAAGGGAAGGGTA30.1142857142857143No Hit
GATGAGGACCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAA30.1142857142857143No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC30.1142857142857143No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG30.1142857142857143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)