FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_D10d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_D10d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9409
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAG2422.5720055266234456No Hit
AAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTC2252.3913274524391537No Hit
AAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAA1691.7961526198320759No Hit
AGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTA1511.6048464236369433No Hit
AGCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGG1291.3710277393984485No Hit
AACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCA1011.0734403230949092No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCT910.9671591029865022No Hit
GAGCAAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTG890.945902858964821No Hit
GGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGA880.9352747369539803No Hit
AAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAA860.914018492932299No Hit
GAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACT720.7652247847805294No Hit
GTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGAC660.7014560527154852No Hit
GCAAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGG530.5632904665745563No Hit
CACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCT510.5420342225528749No Hit
AGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAA510.5420342225528749No Hit
CAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACA500.5314061005420342No Hit
GGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGA490.5207779785311936No Hit
GTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGC420.44638112445530875No Hit
AAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACCT410.4357530024444681No Hit
CTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTG410.4357530024444681No Hit
GTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTCT390.41449675842278666No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT370.3932405144011053No Hit
AGCAAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGG370.3932405144011053No Hit
GCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGG350.37198427037942394No Hit
AAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTCTCTTA340.36135614836858326No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA310.3294717823360612No Hit
GCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAAC300.31884366032522055No Hit
TGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTC290.30821553831437987No Hit
GGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGC280.29758741630353913No Hit
GAGCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTG280.29758741630353913No Hit
ATTAGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTC280.29758741630353913No Hit
TATTAGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGT270.2869592942926985No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTACCTGTCT260.27633117228185783No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGTCTTGGCTGTTTTGCCCA260.27633117228185783No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTGTCTCTTA250.2657030502710171No Hit
GAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCA240.2550749282601764No Hit
GAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTT240.2550749282601764No Hit
GAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACC220.23381868423849508No Hit
ACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAA220.23381868423849508No Hit
GGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAG200.2125624402168137No Hit
AAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCCT190.201934318205973No Hit
AGCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCTGTCTCT190.201934318205973No Hit
GAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTCTCTT180.19130619619513234No Hit
CATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCA180.19130619619513234No Hit
AACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTCTCTTAT180.19130619619513234No Hit
GTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCTGTCTCTTATA180.19130619619513234No Hit
CGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGC170.18067807418429163No Hit
TTAGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCC170.18067807418429163No Hit
AAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCTGTCTCTTATA170.18067807418429163No Hit
AAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCCTGTCTC160.17004995217345095No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCCTGTCTCT160.17004995217345095No Hit
AACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACCTG140.14879370815176957No Hit
GACCTTGTTTCTACTAATAACCCGAAACAAGGTCTTGGCTGTTTTGCCCA140.14879370815176957No Hit
GCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCT140.14879370815176957No Hit
TGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATG140.14879370815176957No Hit
GTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCGCTCTGTCTCT130.13816558614092891No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTCTG130.13816558614092891No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTACTCTGTC120.1275374641300882No Hit
GAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCA120.1275374641300882No Hit
GTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTCTGTCTCTTAT120.1275374641300882No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTGTCTCTT110.11690934211924754No Hit
GCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTTG110.11690934211924754No Hit
AGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTC110.11690934211924754No Hit
AGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTCTGTCTCTTA100.10628122010840685No Hit
GCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCG100.10628122010840685No Hit
GTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGAC100.10628122010840685No Hit
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT100.10628122010840685No Hit
AGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTACCTGTCTCTTAT100.10628122010840685No Hit
AAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCCTG100.10628122010840685No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCC100.10628122010840685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)