FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_C8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_C8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377399
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGCCT47091.2477510539243613No Hit
GTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTG39211.038953468345173No Hit
CATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATC38481.0196105448080148No Hit
CTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGC37861.0031823083791955No Hit
GTATTTATGTGTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTT26940.7138333699877317No Hit
TAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACT25280.6698480918073445No Hit
GGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAG23420.6205633825208864No Hit
ATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACGCA22960.6083746909769236No Hit
CACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATT22920.6073148047557095No Hit
AATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTA22200.5882368527738547No Hit
CTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCG20950.5551154083609124No Hit
CCTGTGATGCGCCCACCTCGGCCTCTCGAAGTGCTGGGATGACGGGCGTG19790.5243787079457021No Hit
CTATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACG18700.4954968084176164No Hit
ATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCTC18180.4817182875418323No Hit
GTATAACACAAACACCTACATCTATCATACAGAAGTCTATTTCCATACAA18130.4803934297653147No Hit
ACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGA15910.4215697444879292No Hit
GTGCTGGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGACTCATTT14490.38394378363482684No Hit
CTCCTGTGATGCGCCCACCTCGGCCTCTCGAAGTGCTGGGATGACGGGCG12470.33041952946351205No Hit
GCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAA12420.3290946716869944No Hit
GTCTATTTCCATACAACCGATACGTATTTACCATACGCAAGAGTATTCAA12210.3235302690256201No Hit
GTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAATACGTATCGGTTG11920.3158460939218175No Hit
GAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGG11780.312136492147568No Hit
GTATAGAGCAGGCACGACTTGCACGGGCATATTCACGAGTCGTTCATGAA11710.3102816912604432No Hit
ATGTAGGTGTTTGTGTTATACAAATAAATACACATCGCTCTATAAAGAAG11650.30869186192862197No Hit
GCTCTATACCGCACGCTGGATCAAGAAATGCAAGCGGCGGTGAGCCTCTA11620.3078969472627114No Hit
CCATACAACCGATACGTATTTACCATACGCAAGAGTATTCAACGCAGAGA11540.30577717482028305No Hit
CATTTACACACATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTT11470.30392237393315824No Hit
AAAGCAGGCTGGTCTCCAACTCCCGACCTCCTGTGATGCGCCCACCTCGG11220.2972980850505698No Hit
GAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAA11200.29676814193996276No Hit
CATTAATACATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGA10740.284579450396No Hit
TCTTTATAGAGCGATGTGTATTTATTTGTATAACACAAACACCTACATCT10690.28325459261948227No Hit
ACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTTCATACGTAA10170.2694760717436983No Hit
AAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAA10160.2692111001883948No Hit
GTGTTATACAAATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATA9950.26364669752702047No Hit
ATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACGCAAGA9890.26205686819519924No Hit
AATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTT9200.2437738308792551No Hit
CTTCTTTATAGAGCGATGTGTATTTATTTGTATAACACAAACACCTACAT9020.2390043428837914No Hit
GGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGG9020.2390043428837914No Hit
GAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAAAACAGAG8980.2379444566625773No Hit
CTATTAGGATCTGAGGTGGTGGAGGGCTTCTTGGACTTCTTCTTCTTCTT8970.2376794851072737No Hit
ACATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGT8960.2374145135519702No Hit
GTATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTTG8880.23529474110954188No Hit
CACAAACACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATA8700.23052525311407823No Hit
GAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGA8550.22655067978452512No Hit
ATGCAGGACAGCTTGTAGCATCCTCCAGTCGACCAGCAGCGGCAGCCGAA8400.22257610645497208No Hit
TCCTAATAGTATGGCAATCGACAACGCCCCTTCTTCCGATACCTTCCTCT8340.22098627712315083No Hit
GAGATAACTACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTT8260.21886650468072255No Hit
CCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAG8070.2138320451299553No Hit
TTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAA8030.21277215890874115No Hit
ACGTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAATACGTATCGGT7990.211712272687527No Hit
AAGTTATACCGCTCGGTAGCAATACAGGTATCGCAGGTGCGTCAAATCAG7920.20985747180040223No Hit
CATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGTC7700.20402809758372437No Hit
AGCGAAAGCAGGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAA7690.20376312602842084No Hit
TCTGTATTCTGTGTTCGCAGCTGTTCTCTGAGGATCGTAGGCCAAGAGTG7670.20323318291781378No Hit
CGTATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTT7540.1997885526988678No Hit
GAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAG7450.19740380870113594No Hit
GTGTGAGAGTAGCTGCGTAGTCAAGTTCTTTACAGCTTGATAGTCTGATT7330.19422415003749346No Hit
GTGTAAGATGGAGGAATAGACATAATCCAGCGAGAAGAAGGAGGGGTTTC7290.19316426381627932No Hit
GAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAAAA7190.19051454826324393No Hit
CTGTATGATAGATGTAGGTGTTTGTGTTATACAAATAAATACACATCGCT7130.1889247189314227No Hit
TCTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATA7120.18865974737611918No Hit
CTCCATAATAGTACCAAGAAGAGATTGATGATTGATAGTGTAAGATGGAG7080.187599861154905No Hit
GTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTTTCTTCCTCTC7050.1868049464889944No Hit
CCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTA7050.1868049464889944No Hit
AGCGAAAGCAGGCTGGTCTCCAACTCCCGACCTCCTGTGATGCGCCCACC7030.18627500337838734No Hit
GTATGATAGATGTAGGTGTTTGTGTTATACAAATAAATACACATCGCTCT6930.18362528782535195No Hit
GGAGAGGAAGAAAGAAAACAGAGATAACTACGTACGCTCGTTCATTTACA6890.1825654016041378No Hit
AACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAAT6430.17037671006017505No Hit
ACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTTGTTTGCATATAA6330.16772699450713968No Hit
CTTCTACGCTGTCACAATCATCTTGAAGCGACGAGTGAAGCTTCCCTCCA6260.16587219362001487No Hit
CTTATATGTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGA6230.1650772789541043No Hit
AAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGTCAACAG6220.16481230739880073No Hit
CTCTTGCGTATGGTAAATACGTATCGGTTGTATGGAAATAGACTTCTGTA6140.16269253495637243No Hit
AATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCT6040.16004281940333703No Hit
GGCCAAGAGTGCGCCGAAGTCGGTTGTCGAGCTAGAGGCAGGAGGGACTT6010.15924790473742645No Hit
GAGATACACGTTGTCGTTGTTTGCATATAAGCGTACAGAAACGTTTACAT5920.15686316073969458No Hit
GTATAACTTCCCGCTCATTCGCACTGAGACCATTTCCTCTGAGTGTATAT5880.15580327451848044No Hit
CAGGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAA5810.1539484736313557No Hit
GTCCTGCATCGAGCTTGACTGCTTTCGTCGTAGAGGGTTGCATGCTTTCA5800.15368350207605214No Hit
GAATACAGATATACACTCAGAGGAAATGGTCTCAGTGCGAATGAGCGGGA5770.15288858741014152No Hit
GAATACTCTTGCGTATGGTAAATACGTATCGGTTGTATGGAAATAGACTT5740.1520936727442309No Hit
CATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCAT5720.15156372963362383No Hit
CCTTTGCATCGTGTGAGAGTAGCTGCGTAGTCAAGTTCTTTACAGCTTGA5650.14970892874649908No Hit
ATGCAAAGGAGTTAGATGTAGCGGGCATAGCGGATCATCTGATAGAGCAG5600.14838407096998138No Hit
GAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAAC5580.1478541278593743No Hit
GAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAA5520.14626429852755307No Hit
CTCTCACACGATGCAAAGGAGTTAGATGTAGCGGGCATAGCGGATCATCT5480.1452044123063389No Hit
GTCTCCAACTCCCGACCTCCTGTGATGCGCCCACCTCGGCCTCTCGAAGT5470.14493944075103538No Hit
GCTATATACTCAAGTAGCGTGTAGGTAAACGCACGGATGTCCACGGCAGC5410.14334961141921415No Hit
GTGCAGGGCCAAGCATTCTCATTCACTCATCTCCTCTTTATTTCCCGCGT5370.142289725198No Hit
CTCCAACTCCCGACCTCCTGTGATGCGCCCACCTCGGCCTCTCGAAGTGC5330.14122983897678584No Hit
ACACAAACACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGAT5300.14043492431087523No Hit
GGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAA5220.13831515186844692No Hit
GAAGAAAAACACGAAGGAGAGGAAGAAAGAAAACAGAGATAACTACGTAC5190.1375202372025363No Hit
AGCGTACGTAGTTATCTCTGTTTTCTTTCTTCCTCTCCTTCGTGTTTTTC5090.13487052164950092No Hit
GTAGAAAGGTTTGCGATTAGAAAGCCTTCGCGTAGCTTGTCGGGCGGCCT5060.1340756069835903No Hit
CTCGAAGTGCTGGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGAC5020.13301572076237614No Hit
CTACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTTCATACGT5010.13275074920707264No Hit
GGTAAATACGTATCGGTTGTATGGAAATAGACTTCTGTATGATAGATGTA4990.13222080609646555No Hit
ATAAAGACGTTTACTTTACGTATGAAAAGCCTCGTATTTATGTGTGTAAA4890.12957109054343016No Hit
ACCGATACGTATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGT4880.1293061189881266No Hit
CTCAAGTAGCGTGTAGGTAAACGCACGGATGTCCACGGCAGCACGCCAAT4850.12851120432221602No Hit
AAAGAAAACAGAGATAACTACGTACGCTCGTTCATTTACACACATAAATA4830.12798126121160894No Hit
ACACGAAGGAGAGGAAGAAAGAAAACAGAGATAACTACGTACGCTCGTTC4820.12771628965630538No Hit
CGTTTACTTTACGTATGAAAAGCCTCGTATTTATGTGTGTAAATGAACGA4810.12745131810100185No Hit
GTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTC4790.1269213749903948No Hit
TATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTTGT4780.12665640343509124No Hit
CTCTTGGCCTACGATCCTCAGAGAACAGCTGCGAACACAGAATACAGATA4780.12665640343509124No Hit
GATAACTACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTTCA4770.1263914318797877No Hit
CACTGAAAGTGTGACGCCGCAGGACTTCGTCAACGTCGACCTCGACTTTT4750.12586148876918063No Hit
CTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATAC4690.1242716594373594No Hit
ATCAGGGGCAGCGCACGACGCGACTTCTATGTTTGGTCGTACGGCACGCG4680.12400668788205586No Hit
GTAAATACGTATCGGTTGTATGGAAATAGACTTCTGTATGATAGATGTAG4670.12374171632675232No Hit
GTGTTTGTGTTATACAAATAAATACACATCGCTCTATAAAGAAGGGATCG4670.12374171632675232No Hit
GTATTTATTTGTATAACACAAACACCTACATCTATCATACAGAAGTCTAT4640.12294680166084172No Hit
CTCCCGACCTCCTGTGATGCGCCCACCTCGGCCTCTCGAAGTGCTGGGAT4640.12294680166084172No Hit
ATACAAATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGAC4630.12268183010553818No Hit
CTCTCGAAGTGCTGGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTG4620.12241685855023464No Hit
ACTGTATGTCTGTCCTTGCCTGAGATCCTAGATTTCAGCGATCACAACTC4600.12188691543962756No Hit
TCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGA4600.12188691543962756No Hit
GAAAAACACGAAGGAGAGGAAGAAAGAAAACAGAGATAACTACGTACGCT4570.12109200077371696No Hit
GTGTATATCTGTATTCTGTGTTCGCAGCTGTTCTCTGAGGATCGTAGGCC4540.12029708610780633No Hit
GATATACACTCAGAGGAAATGGTCTCAGTGCGAATGAGCGGGAAGTTATA4540.12029708610780633No Hit
CACATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGA4500.11923719988659219No Hit
AGAGTATTCAACGCAGAGATACACGTTGTCGTTGTTTGCATATAAGCGTA4470.11844228522068156No Hit
ATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTA4470.11844228522068156No Hit
ATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGTCA4470.11844228522068156No Hit
GATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGACTCATTTCGCTTTT4440.11764737055477094No Hit
CTCCTGTACCTCCTATGGCATACACTGAAAGTGTGACGCCGCAGGACTTC4430.1173823989994674No Hit
TTCCTGAAGAGCAAACAAAGGACTACGCGCAGGCAGTTGCGATTGAAATT4400.1165874843335568No Hit
ATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATCCCTTC4380.11605754122294971No Hit
AAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCG4360.11552759811234263No Hit
GCGTAGAAGTGTTGCATCTCTTCATTCGACTCAGCTAGGCACGGCGCAGA4360.11552759811234263No Hit
GTAGCAATACAGGTATCGCAGGTGCGTCAAATCAGCCCGAACTGTCGGAG4350.11526262655703912No Hit
TGGTAAATACGTATCGGTTGTATGGAAATAGACTTCTGTATGATAGATGT4280.11340782566991435No Hit
GCCCTGCACACATCATCAGTCTTTCATTGTAGCTGAAACGGAAGTAGAGG4260.11287788255930727No Hit
GCATATAAGCGTACAGAAACGTTTACATTAATACATATAAGTAAACGCGT4240.11234793944870018No Hit
GTATATAGCTGAGAAAAAGGCGCCAGGACAAACCCTACATCGCTTCTCTA4200.11128805322748604No Hit
GACATACAGTCGTGCATGAGCTCAACAGACTACGCAATGCGAAATCTACA4070.10784342300854002No Hit
CTACTTGAGTATATAGCTGAGAAAAAGGCGCCAGGACAAACCCTACATCG4040.10704850834262943No Hit
CAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAATAC4030.10678353678732588No Hit
GTATTGCTACCGAGCGGTATAACTTCCCGCTCATTCGCACTGAGACCATT4000.10598862212141526No Hit
ATCTACAATCCTGATATGGTGTCGCTACGCCCGCCGACCCGAAGCAGCCG3990.10572365056611172No Hit
TCTCTGCGTTGAATACTCTTGCGTATGGTAAATACGTATCGGTTGTATGG3990.10572365056611172No Hit
TAGTTATCTCTGTTTTCTTTCTTCCTCTCCTTCGTGTTTTTCTTCCTTCC3990.10572365056611172No Hit
GAAATAGACTTCTGTATGATAGATGTAGGTGTTTGTGTTATACAAATAAA3980.1054586790108082No Hit
CTCGTAGAGGGATAGAAGTAAGTCATGTACAAGCTACGCCGCCAGCATCT3960.10492873590020112No Hit
GGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAAAACAGA3950.10466376434489758No Hit
GGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGACTCATTTCGCTT3920.10386884967898695No Hit
GCAGGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGA3890.10307393501307635No Hit
AACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGG3880.10280896345777281No Hit
ATCTGATAGAGCAGGAGGAAGAAGTGGGAAGCACAGTCAAATCTGATGGT3880.10280896345777281No Hit
CACCTCGGCCTCTCGAAGTGCTGGGATGACGGGCGTGAGCCACCGTGCCC3870.10254399190246927No Hit
TTATAGAGCGATGTGTATTTATTTGTATAACACAAACACCTACATCTATC3860.10227902034716572No Hit
GGAAGAAAGAAAACAGAGATAACTACGTACGCTCGTTCATTTACACACAT3850.10201404879186218No Hit
GTACAGGAGGATAGCATACGATATCTGAGCCAGAATCAGAGTCCTCCTCA3830.10148410568125513No Hit
GTGTATTTATTTGTATAACACAAACACCTACATCTATCATACAGAAGTCT3820.10121913412595158No Hit
GGTCTCCAACTCCCGACCTCCTGTGATGCGCCCACCTCGGCCTCTCGAAG3820.10121913412595158No Hit
ATCTATGCCTCTGCGCCGTGCCTAGCTGAGTCGAATGAAGAGATGCAACA3810.10095416257064804No Hit
CTGTATGTCTGTCCTTGCCTGAGATCCTAGATTTCAGCGATCACAACTCC3800.1006891910153445No Hit
GTGATGCGCCCACCTCGGCCTCTCGAAGTGCTGGGATGACGGGCGTGAGC3790.10042421946004096No Hit
TATTTGTATAACACAAACACCTACATCTATCATACAGAAGTCTATTTCCA3790.10042421946004096No Hit
ATATGTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAA3780.10015924790473742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)