FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_C4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_C4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5939983
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC195400.3289571704161443No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG142230.23944512972511872No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA130420.21956291794101093No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT120230.2024079866895242No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT116860.19673456977907178No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT110800.18653252037926707No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG109110.18368739439153278No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA108220.18218907360509282No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT104880.1765661618896889No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT102510.17257625148085443No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT97090.1634516462420852No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG93440.15730684751117974No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG90270.1519701319010509No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG90240.15191962670600237No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC82090.1381990487178162No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC81170.1366502227363277No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG75190.12658285385665247No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC73600.12390607851907992No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC73590.12388924345406375No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG73320.12343469669862692No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA71970.1211619629214427No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT71970.1211619629214427No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG71850.12095994214124854No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG68290.11496665899548872No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA65540.11033701611603938No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT65500.11026967585597468No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC64040.1078117563636125No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT64040.1078117563636125No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT63340.10663330181247994No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT63330.10661646674746375No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC63220.10643128103228579No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC62990.10604407453691365No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG62330.10493296024584582No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC62150.10462992907555459No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG61960.10431006284024719No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT61810.10405753686500449No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA60620.10205416412807915No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC60360.10161645243765849No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG60030.10106089529212457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)