FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_C10d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_C10d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5373203
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC343580.6394323832544574No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT245980.4577902602972566No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA242560.451425341644453No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC226500.42153627919883174No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT225480.41963797012694287No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA205050.3816159560694059No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA188500.35081496083434777No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA178590.33237158543982054No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA158030.29410763002998397No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT154350.28725882867258135No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA150000.2791630988071733No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT149490.2782139442712289No Hit
GTCATAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACT149400.27804644641194465No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC147240.2740264977891213No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC132740.24704073157109457No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC131580.2448818702736524No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT126640.23568809888626951No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT123250.22937901285322737No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT122300.2276109798941153No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA121410.2259546121745261No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT120640.2245215749339826No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG115320.21462059036295483No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT104860.19515361693946795No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA104700.19485584296740696No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA103980.19351586009313254No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT100590.18720677406009043No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG98710.18370792988837384No Hit
GCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGTAG97250.18099074239331736No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG93690.17436527151496045No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG93670.17432804976845281No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG91150.1696381097084923No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG85160.1584901966294592No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA84430.15713160288193095No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC83550.15549384603559552No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT83270.15497274158448882No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC81920.15246027369522425No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT80320.1494825339746144No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT79810.14853337943867No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA79440.14784477712827898No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT78550.14618840940868974No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG77520.14427148946354718No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC77510.14425287859029334No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC77410.14406676985775524No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG77270.1438062176322019No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG77000.14330372405434896No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA76560.14248484563118127No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA72390.13472411148434182No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG71610.13327246337054452No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA70420.13105776945334097No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA69930.1301458366639042No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA69850.1299969496778737No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG69410.12917807125470598No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG68120.1267772686049643No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG68000.12655393812591856No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT65640.12216177203801903No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC65600.12208732854500379No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC64880.12074734567072937No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC64340.11974235851502353No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG64330.11972374764176973No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA64040.11918403231740919No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA63720.11858848437328721No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT62330.11600157299100741No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA61600.11464297924347916No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA61070.11365660296102716No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT60720.11300522239714374No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT60070.111795515635646No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA59800.1112930220577931No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC59750.11119996769152403No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT58680.10920860425336619No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA58540.10894805202781284No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA58320.10853861281622898No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA58220.10835250408369086No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT57910.10777556701282272No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG57890.10773834526631508No Hit
GTCCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA57850.10766390177329983No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA57600.10719862994195455No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT57040.1061564210397411No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT56570.10528170999681197No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA56400.10496532515149716No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG56390.10494671427824336No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA56140.10448144244689805No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG54840.10206202892390256No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA54150.10077787866938956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)