FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA70.3146067415730337No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC70.3146067415730337No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA70.3146067415730337No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT70.3146067415730337No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA70.3146067415730337No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC70.3146067415730337No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT60.2696629213483146No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT60.2696629213483146No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG60.2696629213483146No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC50.22471910112359553No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACT50.22471910112359553No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT50.22471910112359553No Hit
GTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGC50.22471910112359553No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT50.22471910112359553No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC50.22471910112359553No Hit
CTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACT50.22471910112359553No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC50.22471910112359553No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT50.22471910112359553No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC50.22471910112359553No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA50.22471910112359553No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC50.22471910112359553No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT50.22471910112359553No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT50.22471910112359553No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT50.22471910112359553No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT50.22471910112359553No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC40.1797752808988764No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG40.1797752808988764No Hit
CAACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAA40.1797752808988764No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG40.1797752808988764No Hit
CTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATT40.1797752808988764No Hit
CTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCG40.1797752808988764No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC40.1797752808988764No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC40.1797752808988764No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG40.1797752808988764No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC40.1797752808988764No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG40.1797752808988764No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG40.1797752808988764No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT40.1797752808988764No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC40.1797752808988764No Hit
GCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC40.1797752808988764No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG40.1797752808988764No Hit
GCAGATACTGGGCCATAAGGACCAGGAGTGGAGGAAATACCAATCAACAA30.1348314606741573No Hit
TAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTT30.1348314606741573No Hit
ACTACCACCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAG30.1348314606741573No Hit
CTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGT30.1348314606741573No Hit
CTTTTGATCTCGGCGGAGCCGATCAAGGAATGGGGCATCACCCAATCCAT30.1348314606741573No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTC30.1348314606741573No Hit
AGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC30.1348314606741573No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC30.1348314606741573No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG30.1348314606741573No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT30.1348314606741573No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.1348314606741573No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA30.1348314606741573No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC30.1348314606741573No Hit
GTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGAACAGAT30.1348314606741573No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG30.1348314606741573No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG30.1348314606741573No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC30.1348314606741573No Hit
CGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTCTTT30.1348314606741573No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT30.1348314606741573No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC30.1348314606741573No Hit
CTGCCTGTGTGTATGGGCTTGCAGTAGCAAGTGGGCATGACTTTGAAAGG30.1348314606741573No Hit
AGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGC30.1348314606741573No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA30.1348314606741573No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT30.1348314606741573No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG30.1348314606741573No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT30.1348314606741573No Hit
CTTCTTGACTCAAGGGGCCTTGCTAAATGACAAACATTCCAATGGAACCA30.1348314606741573No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT30.1348314606741573No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT30.1348314606741573No Hit
TCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAA30.1348314606741573No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC30.1348314606741573No Hit
ATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCA30.1348314606741573No Hit
AGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTA30.1348314606741573No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC30.1348314606741573No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG30.1348314606741573No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG30.1348314606741573No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT30.1348314606741573No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT30.1348314606741573No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT30.1348314606741573No Hit
GTCTTTAATGGTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAG30.1348314606741573No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT30.1348314606741573No Hit
ATGAGGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT30.1348314606741573No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG30.1348314606741573No Hit
ACATCCAAATGTGCACTGAACTCAAACTCAGTGATTATGATGGACGACTA30.1348314606741573No Hit
CTCACAGACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAAAC30.1348314606741573No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG30.1348314606741573No Hit
GACCAGCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACC30.1348314606741573No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG30.1348314606741573No Hit
CAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCT30.1348314606741573No Hit
ACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACT30.1348314606741573No Hit
GCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATG30.1348314606741573No Hit
GTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAA30.1348314606741573No Hit
CTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATA30.1348314606741573No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC30.1348314606741573No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG30.1348314606741573No Hit
GCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA30.1348314606741573No Hit
TCAGCAATCTGTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGT30.1348314606741573No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.1348314606741573No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA30.1348314606741573No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC30.1348314606741573No Hit
CCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTG30.1348314606741573No Hit
GCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAAT30.1348314606741573No Hit
GGGTAACACGTTCCATTGTCTGAACTAGGTGTTTCCACAATGTAGGACCA30.1348314606741573No Hit
GGCTGCACACTGATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCC30.1348314606741573No Hit
CATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTC30.1348314606741573No Hit
TCTTTCAGACCAGCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGC30.1348314606741573No Hit

[FAIL]Adapter Content

Adapter graph