FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC100.4789272030651341No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT70.33524904214559387No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT60.28735632183908044No Hit
GACCAACACTATACTCGGGTGCGGGGAAAGGGTGGTCAGCACACAGTGGG60.28735632183908044No Hit
GTGTGGGCCAGCTTCACCACGGTGTGCCGGTGAATGCTGCAGTGGCTCTC50.23946360153256704No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA50.23946360153256704No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA50.23946360153256704No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA50.23946360153256704No Hit
GGCATATAAGATGCACTCCAGATGAAAGCACATCATCCTCACTTCACACC50.23946360153256704No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC50.23946360153256704No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA40.19157088122605362No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA40.19157088122605362No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.19157088122605362No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT40.19157088122605362No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC40.19157088122605362No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT40.19157088122605362No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGCT40.19157088122605362No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT40.19157088122605362No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT40.19157088122605362No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC40.19157088122605362No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC30.14367816091954022No Hit
AACCAGGATCCTGTGTCTCCCTCCCCTTCAGGAGCTGCCTCGCTCGGATA30.14367816091954022No Hit
GTAGAATAGGACTGTCACCCTCGCGACTAGAAAGAAACTGAGCCCCAGAG30.14367816091954022No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT30.14367816091954022No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA30.14367816091954022No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACCTGTC30.14367816091954022No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA30.14367816091954022No Hit
GTGTACCCCAAGGCTCAGGGTGACCTGCCCAGGTCATAGCCAGTGAGTAG30.14367816091954022No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA30.14367816091954022No Hit
CTTAAATACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAA30.14367816091954022No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA30.14367816091954022No Hit
GAATAGGACTGTCACCCTCGCGACTAGAAAGAAACTGAGCCCCAGAGTGG30.14367816091954022No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC30.14367816091954022No Hit
GTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATTTGAGTCAGTC30.14367816091954022No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC30.14367816091954022No Hit
GGGCTACCCCTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGA30.14367816091954022No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA30.14367816091954022No Hit
CATTGGGAATGCTTATCGAAGGACTTAGTATCCGAGCGAGGCAGCTCCTG30.14367816091954022No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTT30.14367816091954022No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG30.14367816091954022No Hit
CATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATC30.14367816091954022No Hit
TGCTAGAGCCTGGCATATAAGATGCACTCCAGATGAAAGCACATCATCCT30.14367816091954022No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA30.14367816091954022No Hit
AATCACATGTGTGTGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGG30.14367816091954022No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG30.14367816091954022No Hit
CTTCACACCTGTGTACCCCAAGGCTCAGGGTGACCTGCCCAGGTCATAGC30.14367816091954022No Hit
GTATAGTGTTGGTCTCTGTGATCAGCATGCTCACCTCCAGGTCACAGTAG30.14367816091954022No Hit
GAGTGGGGAAGTGAGTGACTCTGCCCAGCACTTCCTGAATTCCAGCCAGG30.14367816091954022No Hit
CTATTATTATCTTCATTGTCTAATTTGATATTCTTGACTACCTAGAAAAG30.14367816091954022No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC30.14367816091954022No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30.14367816091954022No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC30.14367816091954022No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTGCATATGTATCCTAT30.14367816091954022No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT30.14367816091954022No Hit
CTTTCCAGCTGGGCGGGAGAGCCACTGCAGCATTCACCGGCACACCGTGG30.14367816091954022No Hit
GGTCAGCACACAGTGGGCACATTGGGAATGCTTATCGAAGGACTTAGTAT30.14367816091954022No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT30.14367816091954022No Hit
ATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCC30.14367816091954022No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT30.14367816091954022No Hit
GACTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGA30.14367816091954022No Hit

[FAIL]Adapter Content

Adapter graph