FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC210.6169212690951821No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT150.4406580493537015No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC130.381903642773208No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC90.26439482961222094No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT90.26439482961222094No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA80.23501762632197415No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA80.23501762632197415No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA80.23501762632197415No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA80.23501762632197415No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC80.23501762632197415No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT80.23501762632197415No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA70.2056404230317274No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGCT70.2056404230317274No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGCTCTTAG70.2056404230317274No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC70.2056404230317274No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG60.17626321974148063No Hit
GAATAGGACTGTCACCCTCGCGACTAGAAAGAAACTGAGCCCCAGAGTGG60.17626321974148063No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG60.17626321974148063No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA60.17626321974148063No Hit
GGCCCACACCTATCAAAGCCCCCTGCTCTACTGTGACCTGGAGGTGAGCA60.17626321974148063No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT60.17626321974148063No Hit
GCTCTACTGTGACCTGGAGGTGAGCATGCTGATCACAGAGACCAACACTA60.17626321974148063No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA60.17626321974148063No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC60.17626321974148063No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC50.14688601645123384No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT50.14688601645123384No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG50.14688601645123384No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA50.14688601645123384No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA50.14688601645123384No Hit
TTCCCAATGTGCCCACTGTGTGCTGACCACCCTTTCCCCGCACCCGAGTA50.14688601645123384No Hit
TCCAGAAACTGATTGCCCCCAGCTCTTAGTTTGCATAGTTTCCCGTTATG50.14688601645123384No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA50.14688601645123384No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC50.14688601645123384No Hit
CTTCACACCTGTGTACCCCAAGGCTCAGGGTGACCTGCCCAGGTCATAGC50.14688601645123384No Hit
CTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACC50.14688601645123384No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT50.14688601645123384No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG50.14688601645123384No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC40.11750881316098707No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG40.11750881316098707No Hit
GTCTAATTTGATATTCTTGACTACCTAGAAAAGAAAGTAGAATAGGACTG40.11750881316098707No Hit
GTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCC40.11750881316098707No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA40.11750881316098707No Hit
TGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCACTGGA40.11750881316098707No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA40.11750881316098707No Hit
GAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACG40.11750881316098707No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATG40.11750881316098707No Hit
TGCTAGAGCCTGGCATATAAGATGCACTCCAGATGAAAGCACATCATCCT40.11750881316098707No Hit
GCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAGT40.11750881316098707No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT40.11750881316098707No Hit
GTATAGTGTTGGTCTCTGTGATCAGCATGCTCACCTCCAGGTCACAGTAG40.11750881316098707No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA40.11750881316098707No Hit
GGTCATAGCCAGTGAGTAGAAAAGCCACTCAAACCAGGATCCTGTGTCTC40.11750881316098707No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTC40.11750881316098707No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA40.11750881316098707No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC40.11750881316098707No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.11750881316098707No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA40.11750881316098707No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA40.11750881316098707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)