FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_B11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759946
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT3264525.6676225784061165No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG1791443.110168046714327No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC1565912.7186192370553472No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC1017611.7667005905958144No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC990051.7188529198016789No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC881111.529719202228632No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA859201.4916806511727714No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA775091.345654976626517No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT592901.029349927933352No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC565080.9810508640185168No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG499680.8675081328887458No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT444720.7720905716824429No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG437130.7589133648127951No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA428800.7444514236765414No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA413140.7172636687913393No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC391690.6800237363336392No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG368350.639502523113932No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA367760.6384782079554218No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC365280.6341726120349045No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA352380.6117765687386653No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT338190.587140921112802No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT319290.5543281134927307No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG309040.5365328077728506No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG298040.5174354065124915No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC287320.49882412092057804No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC271510.47137594692728024No Hit
GTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTT256580.4454555650348111No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC251020.43580269676139327No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC245740.42663594415642087No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT241750.41970879588107246No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG233750.4058197767826296No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA230160.39958707946220323No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG220400.3826424761621029No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG220050.382034831576546No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA214900.3730937755319234No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA206390.35831933146595474No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC205420.3566352879002685No Hit
ATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTGGTAC203890.3539790129976913No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT203630.3535276198769919No Hit
CATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATAT201810.35036786803209613No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA200950.34887479847901354No Hit
GTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT197940.34364905504322435No Hit
GAGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT196460.3410795865100124No Hit
TACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACT194800.3381976150470855No Hit
GTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT193030.335124669571555No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG189190.32845794040430243No Hit
GAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC186720.32416970575765813No Hit
GAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC184180.3197599421939025No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA181800.3156279590121157No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTAT179610.311825840033917No Hit
GGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACA176930.3071730186359386No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA174060.3021903330343722No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC171240.29729445380217107No Hit
AACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGA171020.2969125057769639No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACAT169950.2950548494725471No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA162890.28279779011817124No Hit
AGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGAC161780.28087068871826226No Hit
CATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAG159120.27625258986803No Hit
AAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT156680.27201643904300493No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA150630.26151286834980747No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA150610.2614781458020613No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA148610.2580058910274506No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT143970.2499502599503537No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA142390.24720717867841124No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA142310.24706828848742682No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC141960.24646064390186992No Hit
CTGTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG141020.24482868415780287No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG138230.2399848887472209No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA138000.23958557944814066No Hit
ACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTAT135270.234845951680797No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG134530.23356121741419106No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT128800.2236132074849313No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA122040.211876986346747No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT121610.2111304515702057No Hit
CATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGC116210.2017553636787567No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC113760.19750185157985856No Hit
GTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT112260.1948976604989005No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC110740.19225874687019634No Hit
CTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATA106450.18481076037865635No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT106390.184706592735418No Hit
CTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG105750.18359547120754258No Hit
CATTATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTG105640.18340449719493898No Hit
TAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATA104590.18158156343826834No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG95920.16652933899033082No Hit
CTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAAC94610.16425501211296079No Hit
AAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACA93600.16250152345178237No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA93150.16172026612749493No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG93010.1614772082932722No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG91620.15906399122491774No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA90360.15687647071691296No Hit
GTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATT89170.15481047912601958No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA87490.15189378511534657No Hit
GAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGT87420.1517722561982352No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA86170.14960209696410348No Hit
CAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACC85640.14868194944883162No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTT83540.14503608193549036No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG83470.144914553018379No Hit
AGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTA81740.1419110526383407No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC80420.13961936448709764No Hit
TAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACT79840.13861241060246052No Hit
GCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG79650.1382825463988725No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA79640.1382651851249994No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT78920.13701517340613956No Hit
CTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAG78700.1366332253809324No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT77220.13406375684772046No Hit
TGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTG76830.13338666716667136No Hit
TGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAG75950.13185887506584262No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG75250.13064358589472888No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCATGATAACCTATACAT75140.13045261188212529No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC73380.12739702768046782No Hit
CCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATT72430.12574770666252774No Hit
TTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCT70760.12284837392572778No Hit
ATGTATAGGTTATCATGCGAACAATTCAACTGTCGCCAGTTCATTGGTAC70320.1220844778753134No Hit
GTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGT69510.12067821469159608No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT69150.12005320883216614No Hit
CAATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA67240.1167372055224129No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT65680.11402884679821651No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC63800.11076492731008242No Hit
CAGTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCG63010.10939338667411119No Hit
CTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTC62990.10935866412636508No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA61800.10729267253547169No Hit
GTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACAT61750.10720586616610642No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA61630.10699753087962977No Hit
CTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA61600.10694544705801062No Hit
TGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTG61560.10687600196251841No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCA60880.10569543533915074No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC60700.10538293240943578No Hit
GTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTA58920.10229262566003224No Hit
TAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAGTA57700.10017455024751967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)