FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7981707
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGACAGCAATTGGAGAAGACAAGACCAGCAGCAGGCTTGGGGACA626230.7845815437725289No Hit
CTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAAC538420.6745674828705188No Hit
TCCCAGAGGAGTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAG437820.548529280766633No Hit
CTCTAGGCCTCAGCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTG420570.5269173624138295No Hit
GTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAGAAAGTAGGAG416310.5215801582293111No Hit
GCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGAGTGGTG362850.454602004308101No Hit
GTCCTGCACCACTCAGCCACCTCCCTGCATCACAGGTGTGACCACAGGGA344540.4316620492333282No Hit
GGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACCAAAGGGGCTGGT340920.4271266785413196No Hit
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT326500.40906036766320786No Hit
GTCTTGGGACGCTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCC316650.3967196490675491No Hit
TCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTTCCCAGC295370.3700586854415979No Hit
AACCAAGGGTGTCCAGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAAT291480.3651850412449367No Hit
GTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATTGCTGT284510.35645257336557207No Hit
CACCCAGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACC268700.3366447803709156No Hit
TGCTTAGCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGA262500.32887701841222683No Hit
CCTCTACAGCGTCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTTTCT262410.32876426057734265No Hit
GTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAACAT261570.3277118541184235No Hit
GTGTATGAGGGGGTACTCCAATGCACCGTCAACCCTGTCCTTGGTATTTA254790.31921743055714774No Hit
GTTTGGGTGTGCCAGACTCAAACCAAGGGTGTCCAGGCAAGACAGGGAGG244980.30692682655477077No Hit
GTCCAGGGTTGGTCCCTTACTCGACCCTTCGAACATTTAAGTACAGGAAG217900.27299924690294947No Hit
GATCAGGAAGGGATGACATGCTTAGCCTTATCCCTGTGGTCACACCTGTG214380.2685891626941455No Hit
GCTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAAT209810.26286357041169267No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC209300.26222460934734887No Hit
ACCCTGGACTTGCCAGACTACTGCTAAGTTTTTTGTTTGTTTGTTTCAAG206180.25831567107136355No Hit
GAACATGACCAGCCCCTTTGGTTGGGTTCCCAGCAATGAGCCCAGCCTCT197050.24687701515477828No Hit
ACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGAGTCCAGGGCCAAGGGAAA188940.23671628136688055No Hit
GCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGCA184000.23052712909657044No Hit
CACCTGTGATGCAGGGAGGTGGCTGAGTGGTGCAGGACAGCAATTGGAGA183960.23047701450328856No Hit
CCTGTAGGCCCAGCTACTCGGGAGGCTGAGGCCGGGGAATGGCGTGAACC178290.22337327090558448No Hit
GCCTGAAAGGCTTCCCAGAGGAGTCCAGGGCCAAGGGAAAGTGAGACTGG175070.21933904614639452No Hit
GTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGAG174770.21896318669678053No Hit
CTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCCCCGGCCTCAGCCTCCC164740.20639695243135334No Hit
TCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTC158690.19881712019747155No Hit
CCTGTAATCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCA158330.19836608885793477No Hit
GGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGT154650.19375554627600336No Hit
GCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATT153190.19192636362121535No Hit
GGTCACACCTGTGATGCAGGGAGGTGGCTGAGTGGTGCAGGACAGCAATT152190.19067349878916878No Hit
GCCTACAGGCGCCCGCCACCACGCCCGGCTACTTTGTTCTATTTTTCCTA152050.19049809771268228No Hit
GGCTGGAGTGCGGTGGCGCCATCTCGGCTCACTGCAAGCTCCGCCTCCCG146250.1832314816868121No Hit
CTCCAGGGGCACTAAGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAA141380.17713002995474528No Hit
GGCTTGGGGACACCCAGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATT141220.17692957158161782No Hit
GGGTGGAATGGGCCAGATCAGGAAGGGATGACATGCTTAGCCTTATCCCT136230.1706777760697054No Hit
AGACAGGGAGGTGGCATGGGAAGGGTGGAATGGGCCAGATCAGGAAGGGA135870.17022674473016863No Hit
ATCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTTCCCAG134240.1681845750539327No Hit
GGCCTAGGATGGGGGCTTGGATAGGCGGTGCCTCTACAGCGTCCCAAGAC134110.16802170262576666No Hit
CTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTCCAGACCAT133130.166793895090361No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT132130.16554103025831443No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA131080.16422552218466552No Hit
ATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACCAAAGG128280.1607175006549351No Hit
CCTGTGATGCAGGGAGGTGGCTGAGTGGTGCAGGACAGCAATTGGAGAAG126560.158562573143815No Hit
ATCCAAGCCCCCATCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGG125910.1577482110029847No Hit
GAACAACTCCAGGGGCACTAAGTGGAAGGTGTGCCAGAACTCAGAGCAGC124640.15615707266628556No Hit
AGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACC123570.15481650729599572No Hit
GTATGAGGGGGTACTCCAATGCACCGTCAACCCTGTCCTTGGTATTTATG123190.154340418659818No Hit
GTCTCAGTGGGGCCAGCAAAAGCAAAACTTGTTGGGGTTCTGCTGAGGAG120020.15036883714223034No Hit
GCTTAGCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGAG118210.14810115179622604No Hit
GTGCCTCTACAGCGTCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTT118000.14783805018149626No Hit
GTATTTATGTAAGGAATTCCAGCATTTGCAAATACATTGCTTTCCTAGGA116200.14558289348381243No Hit
GTCTCGCCCTGTCGCCCAGGCTGGAGTGCGGTGGCGCCATCTCGGCTCAC115790.14506921890267332No Hit
CCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATTGCTGTCCTGCACCAC114180.14305210652307834No Hit
CACTGAGACAGTGAGGCCCGCTTCCCCACAGGGGGGCCTTGGTCAAGGGT113150.14176165574607036No Hit
GTCTGGAGCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAGAGTGC112980.14154866872462243No Hit
GTCATGTTCCCCTCCAGGCCTAGGATGGGGGCTTGGATAGGCGGTGCCTC111360.139519027696707No Hit
AGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGA111140.13924339743365674No Hit
ACTCCAGGGGCACTAAGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAA109190.1368003110111659No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG107580.1347831986315709No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT107160.13425699540211133No Hit
CTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAATA106470.1333925186679992No Hit
CTGTAGGGGGAAGGGAGGCAGCGGGGAAGCCACAAAAGCCTACAGCAGGC105020.13157586466153168No Hit
TCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTTTCTCTCAGATGCTC104600.1310496614320721No Hit
CAGTTTGGGTGTGCCAGACTCAAACCAAGGGTGTCCAGGCAAGACAGGGA104330.13071138792741951No Hit
GTCCAGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAATGGGCCAGATC103830.13008495551139623No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC103220.12932070796384784No Hit
GGCATGGGAAGGGTGGAATGGGCCAGATCAGGAAGGGATGACATGCTTAG102940.12896990581087478No Hit
GTAGGGGGAAGGGAGGCAGCGGGGAAGCCACAAAAGCCTACAGCAGGCCG102790.1287819760860678No Hit
CCTCAGCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTC102400.12829335880156964No Hit
TGTCTGATTTGGGGACAGTTTGGGTGTGCCAGACTCAAACCAAGGGTGTC101000.12653934803670444No Hit
AGCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGC98680.12363270162635635No Hit
GGCCAGATCAGGAAGGGATGACATGCTTAGCCTTATCCCTGTGGTCACAC94490.1183831979800812No Hit
CTCCAATGCACCGTCAACCCTGTCCTTGGTATTTATGTAAGGAATTCCAG94280.11812009636535142No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG94080.11786952339894212No Hit
TTCTAGGACTCTACAAGCTGGCAAAGCTGGCCAGAAGCAATTTCTGAAAC94010.11778182286069885No Hit
GGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAATAGAACAAAGTAGCCG91830.1150505775268373No Hit
GGACAGCAATTGGAGAAGACAAGACCAGCAGCAGGCTTGGGGACACCCAG90610.11352208243174049No Hit
AAAGCAGGGAGGTGAAATTGTGCATGTCTGATTTGGGGACAGTTTGGGTG90610.11352208243174049No Hit
GGCTAAGCATGTCATCCCTTCCTGATCTGGCCCATTCCACCCTTCCCATG87690.10986371712216447No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC86850.10881131066324534No Hit
GGATAAGGCTAAGCATGTCATCCCTTCCTGATCTGGCCCATTCCACCCTT85490.10710741449166199No Hit
AGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAATGGGCCAGATCAGGA85110.10663132585548431No Hit
GGTGAAATTGTGCATGTCTGATTTGGGGACAGTTTGGGTGTGCCAGACTC84910.10638075288907498No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG84190.10547869021000145No Hit
GTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGAGTCCAGGG83370.10445134104772325No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT82910.10387502322498184No Hit
CCAATGCACCGTCAACCCTGTCCTTGGTATTTATGTAAGGAATTCCAGCA81880.10258457244797384No Hit
GGAATACCGGGTGCTGTAGGCTTTTTCTTTGGCTTTTTGCTGTTTCTTTC81720.1023841140748464No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC81500.10210848381179616No Hit
CTGTAGGCTTTTTCTTTGGCTTTTTGCTGTTTCTTTCCTTTTCTTCCAGA81490.10209595516347568No Hit
AAGTGGAAGGTGTGCCAGAACTCAGAGCAGCCTGAAAGGCTTCCCAGAGG81170.10169503841722077No Hit
CCCCCATCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTT80900.1013567649125682No Hit
TCACAGGTGTGACCACAGGGATAAGGCTAAGCATGTCATCCCTTCCTGAT80310.10061757466166073No Hit
CTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTCCAGACCATCC80010.10024171521204675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)