FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5982974
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT223300.37322575695632304No Hit
CCGTACACCGAGCACAAGTAGTGGCCTCTGAGTCCAACATTGTTGATATC195930.3274792770284477No Hit
GTGTACGGAGCGCGGCTCATGGTTCCAGCTGGCCGGGGGCTCATCGTGGT182400.30486510554784296No Hit
GTCTATAACTGTGATTATTTAGCTTCTCTCTTATTCAGCCTTCTCAGGGT158850.26550341017694545No Hit
GTCCGTAGCCAGCCTGTCGCACGCAGCTTTGCCCACGCCATAGGGGACAT156160.2610073184339427No Hit
GTTATAGACAACACAGAAGATTCTAGGTGGCACGTTCTTAAAAATCGAGG154480.2581993503565284No Hit
GTCTAGTCTCCAGGTAAATATCATTGCTCCATGCATGCACTTTAGCCATA152600.2550571003651361No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC139540.2332284913823794No Hit
GCTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAAT133920.22383516959960048No Hit
GTACAATGCCTTGCCGGTGGTAGGCCTCAGTAAATGTAGATTTTAAACCT132490.22144505391465846No Hit
TTCCTAACCTCCCCCTCTGACCCTGGAATGCTCATTCCCACTCACCGTTC129500.2164475393006889No Hit
ATCTTACTTTCTGGTACAAATGGAAGATTTGATCCATAAAGAAATTCAAA129210.21596283052542098No Hit
GCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGCA127850.21368971351037125No Hit
GTCTTAAAGCGTTACCATTAAAAAGGAATTATCCTACTTAGGAAAAAAGG127810.21362285712757567No Hit
CCTGTAATCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCA108310.18103037051473064No Hit
TCCCAGCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTC106050.17725298488678037No Hit
CCATAGGGGACATTGAAGAGATACTGTAGCCCCCCAAGGGAGGAGATGAC105000.17549800483839642No Hit
GGTTAGGAATGTGGAGCTTGAATCTAAGTTTAAAGAGTACTGGGACTCCG104130.17404387851259256No Hit
CCTGTAGGCCCAGCTACTCGGGAGGCTGAGGCCGGGGAATGGCGTGAACC103510.1730076045792611No Hit
GGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGT101930.1703667774588357No Hit
CTTTAAGACAGGGAAACATTGGTGGCTGTGTCTAGAGTGCAATGAATCTG101770.17009935192765338No Hit
CTCCAGATCCTTCTCAATATCCAAAAGTAAATAAAATGCAGAGATAGTGG96610.1614748785470236No Hit
GAATAAGAGAGAAGCTAAATAATCACAGTTATAGACAACACAGAAGATTC96200.16078960062336892No Hit
CTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCCCCGGCCTCAGCCTCCC95000.15878390913950152No Hit
AGACTAGACAGTGCCATTCAACTGGATATTAAAGGAACAGTGAGGAGAGT92640.15483938255456234No Hit
GTCTAGAGTGCAATGAATCTGCATGTATTGTCTACCTGCTTGAACAGTGA90830.15181413123306237No Hit
CCCCTATGGCGTGGGCAAAGCTGCGTGCGACAGGCTGGCTACGGACTGTG88670.14820388656210107No Hit
CCACAGGAACACACTGGCCCCCTCGAGATTGTGCCTGGTGAGGGACAGAG88140.14731803949005964No Hit
CTCCGTACACCGAGCACAAGTAGTGGCCTCTGAGTCCAACATTGTTGATA87950.14700047167178062No Hit
GCCTACAGGCGCCCGCCACCACGCCCGGCTACTTTGTTCTATTTTTCCTA87490.14623162326963146No Hit
CTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTCCAGACCAT84950.14198624296211215No Hit
GGCTGGAGTGCGGTGGCGCCATCTCGGCTCACTGCAAGCTCCGCCTCCCG84760.14166867514383316No Hit
GTAAATACACTTGTTTCTTTGACCCTCCTGGGTGAGAGTCTTGGAAGCAT84730.14161853285673648No Hit
GTACTGGGACTCCGGCATAGGCGTTGTTGACCAGCACATCCAGACGCCCT82740.13829242781265638No Hit
GAATTTGAGTAATCAAGGCTTTTCTCTCTCAGGTTTTTCGGACAATTTGA81830.13677144510405695No Hit
ACTATAAACTCATATCCCCTCTGTCCCTCACCAGGCACAATCTCGAGGGG80430.13443147170621167No Hit
GGTAAGGACAGTTCTGCAGGCCCAGGGAAAAGCAGAGAGCTAGTCATGAA77610.1297180967191233No Hit
AGCCAGGAGAGTGAAGTGCAAAGCTTGTTTGAGCAAGTCGATCGGGAACA77390.12935038661374762No Hit
AGATGTTACTTTGTATTCCAGGTGAGAAAACCACCGGGAATGAAGAAAGA76940.12859825230729732No Hit
GTCTCGCCCTGTCGCCCAGGCTGGAGTGCGGTGGCGCCATCTCGGCTCAC75370.12597413928257084No Hit
GTCCCTCACCAGGCACAATCTCGAGGGGGCCAGTGTGTTCCTGTGGTGTG75280.1258237124212808No Hit
GTCTTACACGACTTGCCAAAGAATTTGGGACTTTATTCTGAGGATAACAG75270.1258069983255819No Hit
CAATGGGACAGGGCACCACTGAAACAGGCTAAGGTGCCCAGAATTGTTTC73870.1234670249277366No Hit
GGCTTATAGGAAGTGTAGCCAGGAGCCAGAGGTCTAAGAAGCTTCCTAGG73020.12204632679333055No Hit
GATCTAAGAAGGACCTAAGAGCTCCCTAATCAATTGGTCCTCATTGTTCC72680.1214780475395681No Hit
GCTAAGAGCCATGTAAATACACTTGTTTCTTTGACCCTCCTGGGTGAGAG71650.11975649568258194No Hit
GGATAAGAGATTGCTTCCAGTTTTGAGGAATTATTGAGTCATTCAATTCT70030.11704881217936096No Hit
TGTAAATACACTTGTTTCTTTGACCCTCCTGGGTGAGAGTCTTGGAAGCA70010.11701538398796317No Hit
GTGTTAGGTTTGCACTTTGCAGAGAAAATGAATATGTCATAATCCCAATT68570.11460855420732231No Hit
CTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAATA68360.11425755819764552No Hit
CTATAAGCCAGTTCCTGAGGTTCCCAAAACCTCAGACTGGGCTGTTTTGG67920.11352213798689414No Hit
GTGTAACCCTGAGAAGGCTGAATAAGAGAGAAGCTAAATAATCACAGTTA66910.11183401432130576No Hit
TCCCAGGACCCTGGGATCACAACCAGAGCTGAAGGCAGGCACTTAACCGA66040.1103798879955019No Hit
AGCCTACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGC65830.1100288919858251No Hit
GCCTTATTTGTGCCTTGCCTTCCACTAGCAGAGTACAATGCCTTGCCGGT65580.10961103959335275No Hit
ATGTTACTTTGTATTCCAGGTGAGAAAACCACCGGGAATGAAGAAAGATA65220.10900933214819251No Hit
GACTCACACCGTCCACTTCGTAATGCACATCCCGGAATGACAGGCTGCAA65120.10884219119120357No Hit
ACCCAGGCATTACCTGGCACGGACTGCCTGGGCTGGCCGGGACCCTCCGG64780.10827391193744115No Hit
GGCTACGGACTGTGCCCGGGAGCTGCGGCGCCATGGGGTCAGCTACGTGT64110.1071540675256152No Hit
CCTCTGAGAGGTGAGCTCCCCCACTTTGATGGATGAGAAAACTGAGGTTC63110.1054826579557257No Hit
GATCTGAAAGGTCTTACACGACTTGCCAAAGAATTTGGGACTTTATTCTG62750.10488095051056548No Hit
GTCCTGGGATTGAGCCAGCATCGGGCTCCCTGCTCAGGGGGAAACTGTTT62680.10476395184067322No Hit
TCCCAGTACTTTGGGGTCTGTAGGAAAAGGACCTAGCATTCCTGAAGCTC62600.10463023907508207No Hit
CCCGGGCACAGTCCGTAGCCAGCCTGTCGCACGCAGCTTTGCCCACGCCA62580.10459681088368428No Hit
GTCCTGGGATTGAGCCCCACATTGGGCTCTCTGCTCAGCAGGGAACCTGC62480.10442966992669532No Hit
GAGTAGAGATGATCCCATGCAAGCTGGTGAAATGAGGTGTTATCCAGTGG62450.10437952763959864No Hit
CTGTAGCGTTGTGCAAGGTCACAAGATGGCAGCACCTTCCCACTCAGGCT62020.10366082152454614No Hit
GTGGAGAGAAGTCCACGTGCTGCGAGCTGGCGCTGCGACAGGCACTTCTC61890.10344353828046053No Hit
GTCTTGGAAGCATTGGAATTTGTTAAAGGGAAAAAAGGATCTGTCTGAAG61830.10334325370626717No Hit
TCTTAGACCTCTGGCTCCTGGCTACACTTCCTATAAGCCAGTTCCTGAGG61410.10264126168691357No Hit
ACTTTGGGGTCTGTAGGAAAAGGACCTAGCATTCCTGAAGCTCCTCAAAC60350.1008695675428307No Hit
CGGTGTACGGAGCGCGGCTCATGGTTCCAGCTGGCCGGGGGCTCATCGTG60300.10078599706433625No Hit
CTCCTGGCTTGAATCACACACCACAGGAACACACTGGCCCCCTCGAGATT59980.10025114600197159No Hit
GTGTGTTCCTGTGGTGTGTGATTCAAGCCAGGAGAGTGAAGTGCAAAGCT59950.10020100371487492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)