FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA80.25007814942169426No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC80.25007814942169426No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70.2188183807439825No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT60.1875586120662707No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC60.1875586120662707No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG60.1875586120662707No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT60.1875586120662707No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC60.1875586120662707No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC60.1875586120662707No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT60.1875586120662707No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA50.15629884338855893No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA50.15629884338855893No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC50.15629884338855893No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC50.15629884338855893No Hit
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGT50.15629884338855893No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT50.15629884338855893No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG40.12503907471084713No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC40.12503907471084713No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA40.12503907471084713No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA40.12503907471084713No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA40.12503907471084713No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA40.12503907471084713No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA40.12503907471084713No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA40.12503907471084713No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA40.12503907471084713No Hit
ATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTCCT40.12503907471084713No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTT40.12503907471084713No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT40.12503907471084713No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA40.12503907471084713No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT40.12503907471084713No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC40.12503907471084713No Hit
GTCTTTAATGGTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAG40.12503907471084713No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC40.12503907471084713No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG40.12503907471084713No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40.12503907471084713No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT40.12503907471084713No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT40.12503907471084713No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC40.12503907471084713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)