FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4323
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC130.3007170946102244No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC100.2313208420078649No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA90.2081887578070784No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80.18505667360629194No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA80.18505667360629194No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT80.18505667360629194No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT80.18505667360629194No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA70.16192458940550544No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA70.16192458940550544No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT60.13879250520471895No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT60.13879250520471895No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA60.13879250520471895No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA60.13879250520471895No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG60.13879250520471895No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT60.13879250520471895No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG60.13879250520471895No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT60.13879250520471895No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC60.13879250520471895No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG50.11566042100393245No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT50.11566042100393245No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT50.11566042100393245No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA50.11566042100393245No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA50.11566042100393245No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG50.11566042100393245No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT50.11566042100393245No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT50.11566042100393245No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA50.11566042100393245No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.11566042100393245No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC50.11566042100393245No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG50.11566042100393245No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG50.11566042100393245No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG50.11566042100393245No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC50.11566042100393245No Hit
TTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTGCTCCT50.11566042100393245No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT50.11566042100393245No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.11566042100393245No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC50.11566042100393245No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT50.11566042100393245No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA50.11566042100393245No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT50.11566042100393245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)