FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R2_A11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5224752
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG305590.5848890052580485No Hit
ATCTAAGACTGTCTCTCTTTTAATCACACACACACATGCACACACACAGA195190.3735871099719183No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT193050.36949122178430666No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT172420.33000609406915393No Hit
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA152160.29122913393784045No Hit
GTCTTAGATTCTCCTTCATGAGTATGTCCCTTAGAGGGATAAATGATGAA138360.2648163970270742No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC120310.230269302734369No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC115750.2215416157551593No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA113450.21713949293669824No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT101480.19422931461627269No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT100910.19313835374387148No Hit
ACCTACAACAGTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATC98550.18862139293884186No Hit
CAATAATGGTCAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACA96500.18469776173108313No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG96370.18444894609351792No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC89200.17072580669857632No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT87250.16699357213509847No Hit
ACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACT86370.1653092816654264No Hit
GTAGGAGACAGAGTGGCATTTTGTACAATATTAAATTCAGGTTCTCAATA86070.16473509173258366No Hit
CTCCAGAACCCATCCACATGCCGGTTCTCTGAGTTCCAGAATTTCCCCAG85600.16383552750446337No Hit
GTCCTGGGGAGACACCAAACTGTGTTTTAGTGAGCTTCCTAAGGCTTGAA85040.16276370629649026No Hit
GTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCC84890.16247661133006885No Hit
ATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGAAA83440.15970135998799562No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC82840.15855298012231012No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT81890.1567347120016414No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA81760.15648589636407623No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA80870.15478246622997607No Hit
CTCTAAGGGACATACTCATGAAGGAGAATCTAAGACTGTCTCTCTTTTAA80680.15441881260584234No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC79570.15229430985432418No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG79520.15219861153218373No Hit
GAACTATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTT78790.15080141602893304No Hit
TTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTGCTCCT78100.14948077918339472No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA77890.14907884623040482No Hit
ATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTCCT77410.1481601423378564No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT76970.1473179971030204No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG75520.1445427457609471No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT75260.14404511448581675No Hit
ATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTTCAGTA75150.14383457817710774No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA75030.14360490220397065No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA74870.14329866757312118No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGGCACACCCCAAGT74310.14222684636514804No Hit
GTTCTGGAGACTGAATAGGAAAACCAGAAAAGAGAACATATCAGGCAGAG74000.1416335167678772No Hit
GAATAATGTCCCTCCCCAAAAATGATAGCCCAATTCCTAGAAACTGTGAA73870.14138470113031204No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA73600.14086793019075355No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA72880.13948987435193097No Hit
ATTCTTACCTGTGCATTGGAATCCTTGATGCCTGGTCCCCACCCACCCCC72760.1392601983787939No Hit
ATCTTAAGGTTAGCTGATTGACAACCTTAACTCCATCTTTGACCTAATAC72630.13901138274122868No Hit
AGTTAGGGTTGCTTTGGGCTCCCAGATCACCAACAGATTTCATCATTTAT71860.13753762858026564No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA71470.13679118166757007No Hit
ATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCCTGATATGTTCT71220.13631269005686777No Hit
CTTCTACCCTGTAAGTATCTCTCCGTTCATTGCTGTTAACTGTCCCCCAG70710.1353365671710351No Hit
CAATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTC70120.1342073269697777No Hit
GAGTGATACAATCCCACCTGCGTGTTAGAAAGGATTTTCAAGTGAAGGTT69570.13315464542623265No Hit
CATCTGGATGTATAAAAAAATGCTCATGATCTCAACAGAGGAATGCTCTT67080.12838886898363788No Hit
GTCTAGGGAATATCCTCTAAGGAGTAAGTCATGTTTGTTTAATCGGATCA66300.12689597515824674No Hit
CATATGAACAAGTACTGAATTTTGTCTATGCTCAAATGAGGTGATGTGTG65260.12490545005772523No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT65050.1245035171047353No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG64980.12436953945373866No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT64630.12369965119875545No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG64110.1227043886484947No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC63940.12237901435321714No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG61690.11807258985689656No Hit
GTAGTTGGGTGTTTGCTACTTGCACTGAACTATCCTGATTGATCCTCTAA61550.11780463455490328No Hit
GTTCATATGTTGGGGACATCACCCCCATTCTGAAGAAGTAGAAGGTGGGT61260.11724958428648861No Hit
GTCTAGGAAGTGCAGGGAAATAGAGAAATCAGAAGACCTGAGTTGAACTC61240.11721130495763243No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT60530.11585238878323795No Hit
ATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAAT59310.11351734972301078No Hit
AGACTATACTCTAAGTGGTATTAGGTCAAAGATGGAGTTAAGGTTGTCAA58610.11217757321304438No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA58610.11217757321304438No Hit
CCCTTAGAGGGATAAATGATGAAATCTGTTGGTGATCTGGGAGCCCAAAG58540.11204359556204772No Hit
ATATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGG58490.11194789723990727No Hit
GGGTAGAGGTGGGGAAGGCAGAGATCTGAGAACTCTTTAGGAAGCAGAAT58390.11175650059562635No Hit
TATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGGC58250.11148854529363307No Hit
GGTGTACATAGAATGCTCCACAAATATTGGCTGGAAGGAAATGGTAGCCT57780.11058898106551278No Hit
TAATGGTGGTGTGTCCCTACTTTTAGATAATAACGCACAATAGAGAATGG57720.11047414307894424No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC57560.11016790844809475No Hit
GAACAATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAG57240.10955543918639582No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA57220.10951715985753965No Hit
GTGCATGTGTGTGTGTGATTAAAAGAGAGACAGTCTTAGATTCTCCTTCA57160.10940232187097111No Hit
TTCCTATTCAGTCTCCAGAACCCATCCACATGCCGGTTCTCTGAGTTCCA56900.10890469059584072No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA56870.10884727160255644No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT56290.10773717106572714No Hit
GCCTGATATGTTCTCTTTTCTGGTTTTCCTATTCAGTCTCCAGAACCCAT56170.10750749509259004No Hit
GTCTGGAACAGTGGCAACCATATTGTGTCAGTGAAATGAGGCATCATCCA55640.1064930928779012No Hit
CTCATGGACCAAGCAACGGCACTCTGGGGAACAAACAGGACACAAAAACT55570.10635911522690455No Hit
GTAAGTATCTCTCCGTTCATTGCTGTTAACTGTCCCCCAGCTTTGTTGGA55380.10599546160277082No Hit
CACATGCACACACACAGAAAGGGAGAAAATTAACCCAAAGTAAAAGCAGA55040.10534471301221571No Hit
GGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAATAATGCAGTTG54760.10480880240822915No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT54650.10459826609952012No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT54380.10408149515996165No Hit
CATCAGGAAAGACTACTTTGGGGAGAAAGAACAATAATCACCTGATGTAA54310.10394751750896503No Hit
ATGTACACCAGGCCTTGTGCTGATTGCAAGGGAGACAGAGACCCTGCCCT54230.10379440019354029No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT53850.10306709294527282No Hit
CTTCAACTCAGTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTT53830.10302881361641662No Hit
GGGTATGCTGGAAGCATTTTTATTTCAGTTTAGTATCCATTTCGGGTAGG53770.10291397562984808No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA53480.10235892536143343No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA52880.10121054549574794No Hit
ACCCTAACTAGGGTAATAATTCTGTCTCCTTGCCCCAAGCCTTTCACATT52410.10031098126762764No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)