FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_G7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_G7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286683
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG8050.28079795453514855No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT6900.24068396103012735No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC6770.23614933567738583No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5520.1925471688241019No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT5390.18801254347136034No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4640.16185124335939No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4540.15836307001112726No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4480.15627016600216964No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4270.14894500197081795No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG4170.1454568286225552No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC4130.14406155928325012No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT4080.14231747260911878No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT4040.1409222032698137No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3980.13882929926085608No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3920.13673639525189843No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3850.13429467390811453No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG3840.13394585657328825No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3810.13289940456880947No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT3800.13255058723398316No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA3780.13185295256433063No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG3630.12662069254193656No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3540.12348133652850012No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3480.1213884325195425No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG3470.1210396151847162No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA3430.11964434584541114No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA3400.11859789384093232No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA3340.1165049898319747No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG3280.11441208582301705No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG3250.11336563381853824No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC3100.10813337379614418No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC3010.10499401778270774No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT2990.10429638311305518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)