FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_F10b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_F10b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189588
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA17690.9330759330759331No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT17140.9040656581640188No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC10420.549612844694812No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC10080.5316792202038104No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT9670.5100533789058379No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT9120.48104310399392364No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG8910.469966453573011No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA8230.43409920459100787No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT7920.41774795873156534No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC7710.40667130831065257No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC7150.37713357385488533No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT7030.3708040593286495No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC6730.3549802730130599No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC6660.351288056206089No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG6580.3470683798552651No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT6340.33440935080279344No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT6030.31805810494335085No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG5830.3075089140662911No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA5820.30698145452243814No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC5800.3059265354347322No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG5750.30328923771546723No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG5670.29906956136464335No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA5620.2964322636453784No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC5620.2964322636453784No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC5500.2901027491191426No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA5420.2858830727683187No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT5220.27533388189125896No Hit
GGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAA5170.272696584171994No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA5040.2658396101019052No Hit
GATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAA5000.2637297719264933No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC4970.2621473932949343No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA4880.2574002574002574No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC4850.25581787876869844No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC4840.25529041922484547No Hit
CATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAAC4770.2515982024178745No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT4700.24790598561090366No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT4690.24737852606705066No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC4560.24052155199696182No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT4510.2378842542776969No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT4470.23577441610228497No Hit
ACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTA4450.234719497014579No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA4300.2268076038567842No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA4260.22469776568137226No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA4160.21942317024284236No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA4110.21678587252357745No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA4100.21625841297972445No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT4080.21520349389201848No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT3990.21045635799734158No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3930.20729160073422367No Hit
GTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAA3800.20043462666413486No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA3780.19937970757642887No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA3780.19937970757642887No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT3770.19885224803257592No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAAATTAC3720.19621495031331096No Hit
CACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCG3710.19568749076945802No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG3640.19199527396248708No Hit
TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAA3610.19041289533092812No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATTTCGTCA3580.18883051669936915No Hit
GCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAA3560.18777559761166318No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG3530.1861932189801042No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG3510.18513829989239824No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA3450.18197354262928034No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG3360.17722640673460346No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA3330.1756440281030445No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT3320.1751165685591915No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT3250.17142435175222062No Hit
GTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATA3210.16931451357680866No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG3200.16878705403295566No Hit
GAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGG3170.16720467540139672No Hit
GGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA3150.16614975631369075No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG3120.16456737768213178No Hit
GGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCC3100.1635124585944258No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT3100.1635124585944258No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTATT3100.1635124585944258No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3080.16245753950671984No Hit
GAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAAGTTGA2970.15665548452433697No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC2950.155600565436631No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA2940.15507310589277803No Hit
CATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGG2930.15454564634892504No Hit
ATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATC2930.15454564634892504No Hit
GTCCAAAGTTCTTTCATTTTCCAATAGAACCAACAGTTCGGCATTGTAAG2910.15349072726121907No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA2890.1524358081735131No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA2880.15190834862966013No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG2860.15085342954195413No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA2860.15085342954195413No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT2820.1487435913665422No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA2810.1482161318226892No Hit
AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAAT2800.1476886722788362No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC2790.14716121273498323No Hit
AATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGC2750.1450513745595713No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTCTCTTTTTTAACTAGCCA2740.1445239150157183No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA2720.14346899592801232No Hit
GTTCGGCATTGTAAGTCCAAATGTCCAGGAAACCATCATCAACTTTTTTA2690.14188661729645338No Hit
ACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTG2690.14188661729645338No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT2690.14188661729645338No Hit
GTCCAAATGTCCAGGAAACCATCATCAACTTTTTTATTTAAATTCTCTAT2660.1403042386648944No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG2660.1403042386648944No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA2640.13924931957718842No Hit
GTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGG2630.13872186003333545No Hit
GGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCA2600.13713948140177648No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA2590.1366120218579235No Hit
GACCTGAAGAGCACACAGAATGCCATTGACGAAATTACTAACAAAGTAAA2460.12975504778783467No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG2460.12975504778783467No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA2440.1287001287001287No Hit
GTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAAT2410.12711775006856973No Hit
TGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAA2410.12711775006856973No Hit
GTCCAGGAAACCATCATCAACTTTTTTATTTAAATTCTCTATTCTTTTTT2410.12711775006856973No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC2400.12659029052471676No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT2400.12659029052471676No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC2370.1250079118931578No Hit
GAACCAACAGTTCGGCATTGTAAGTCCAAATGTCCAGGAAACCATCATCA2360.12448045234930481No Hit
GGTAAATCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTT2350.12395299280545183No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT2340.12342553326159884No Hit
CAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCAGTTCA2340.12342553326159884No Hit
GACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCA2320.12237061417389287No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT2310.12184315463003986No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT2310.12184315463003986No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA2280.12026077599848091No Hit
TTGTAAGTCCAAATGTCCAGGAAACCATCATCAACTTTTTTATTTAAATT2270.11973331645462792No Hit
ATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAAT2260.11920585691077494No Hit
GTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGACTCGAACA2250.11867839736692196No Hit
GCTGTGGAGAGTGATTCACACTCTGGATTTCCCAGGATCCAGCCAGCAAT2230.11762347827921599No Hit
GAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACA2230.11762347827921599No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG2220.117096018735363No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTA2220.117096018735363No Hit
CGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAA2210.11656855919151002No Hit
GTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAA2200.11604109964765702No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2180.11498618055995105No Hit
CTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCA2180.11498618055995105No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT2170.11445872101609807No Hit
GTGTTACCCAGGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGA2160.11393126147224508No Hit
TGCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC2160.11393126147224508No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2140.11287634238453911No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA2140.11287634238453911No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG2120.11182142329683312No Hit
GGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAA2100.11076650420912715No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG2080.10971158512142118No Hit
GAATGCCATTGACGAAATTACTAACAAAGTAAATTCTGTTATTGAAAAGA2070.1091841255775682No Hit
ATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGA2060.10865666603371521No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG2060.10865666603371521No Hit
CCTGAAGAGCACACAGAATGCCATTGACGAAATTACTAACAAAGTAAATT2050.10812920648986223No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA2050.10812920648986223No Hit
GTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAAGTTGATG2010.10601936831445029No Hit
ACGGGAAACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAA2010.10601936831445029No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGGCTT2000.1054919087705973No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC2000.1054919087705973No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT2000.1054919087705973No Hit
GTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAACAGTTCGGCAT1990.10496444922674432No Hit
GACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAG1990.10496444922674432No Hit
CTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGCTCCAGCA1990.10496444922674432No Hit
ATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCC1980.10443698968289133No Hit
GGAAAAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGG1980.10443698968289133No Hit
GTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT1980.10443698968289133No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT1970.10390953013903834No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT1970.10390953013903834No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTTCCATTGCGAA1970.10390953013903834No Hit
TATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCC1960.10338207059518535No Hit
GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACACGT1960.10338207059518535No Hit
TATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGA1950.10285461105133237No Hit
CATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTT1950.10285461105133237No Hit
CAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCC1920.1012722324197734No Hit
CGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCT1910.10074477287592042No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)