FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_E2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_E2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852996
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC1068231.360283387384891No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA700890.8925128702472279No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC684950.8722148846121913No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC677650.8629190693590064No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT661990.8429776355419002No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA592710.7547565285911263No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC587370.747956576063454No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA577420.735286252533428No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC513830.6543107878827393No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA512390.6524770928190973No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC403100.5133072778847716No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG376680.4796640670643408No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT375430.47807231787715154No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA374730.4771809383323256No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA374480.47686258849488783No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT361910.46085595866851325No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT351210.4472305856261738No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA336350.42830787128886855No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG334860.4264105062577391No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA321680.40962710282801623No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA316740.40333651004024446No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT309740.39442271459198497No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC308680.3930729112812486No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA301860.38438832771594433No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG298660.38031344979674003No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT290390.3697824371742963No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG289930.3691966734734107No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT276990.3527188858876281No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT270720.3447346719646871No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA269590.3432957306994681No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA254480.3240546665247251No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG244430.3112570030597239No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT243780.31042929348238557No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT240290.30598512975175335No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA239790.3053484300768777No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA237470.3023941435854545No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT236440.3010825422552106No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT233170.2969185263815237No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC228010.2903477857368067No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG225020.28654032168105015No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG211620.26947677039438195No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC210050.2674775334152723No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC208270.2652108825727149No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG207100.26372100533350584No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC206230.2626131478992222No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC205600.2618109063088788No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC203310.2588948217979482No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA202470.25782516634415703No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG197900.25200573131579335No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG196640.25040124813510667No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA196020.2496117405382608No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA193070.2458552124564943No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA189620.24146198469985214No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA189520.24133464476487698No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA186210.2371196929172No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTA185820.23662306717079695No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA182710.23266279519307026No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA180940.23040887834401036No Hit
GGTAAATCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTT178210.22693249811918917No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA173300.22068010731191No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC172920.22019621555900448No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT171230.2180441706579247No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG168340.21436404653714328No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT168050.21399476072571538No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG164970.21007269072848123No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA163730.20849367553478954No Hit
AATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTATATACAT163340.20799704978838648No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT162570.20701653228907793No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG161830.20607421677026194No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG160800.20476261544001806No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG158270.20154091508514713No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT153020.1948555684989525No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT152680.19442261272003702No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC151550.192983671454818No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT150110.19114997639117606No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC148560.18917620739906146No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC148280.1888196555811311No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG145340.1850758614928621No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG145330.18506312749936457No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA145290.18501219152537451No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG144080.1834713783121754No Hit
GTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTA143970.18333130438370274No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT143320.18250359480636436No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA142940.18201970305345883No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT142720.18173955519651352No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA142310.18121746146311546No Hit
GACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAG141640.18036428389878206No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG140420.17881073669208541No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA140320.17868339675711026No Hit
ATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCC140100.17840324890016498No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA137230.17474859276637858No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT134970.17187071023594053No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA133690.17024075906825878No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT133650.17018982309426875No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA131210.16708272868087543No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA129380.16475240787083045No Hit
CTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACT127970.16295691478768104No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTATT127400.16223107715832275No Hit
ACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTG126520.16111048573054157No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG125670.1600280962832529No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC124140.15807979527813334No Hit
AGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTG123680.1574940315772477No Hit
TGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAA122330.1557749424550834No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT119790.15254050810671493No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA118510.1509105569390332No Hit
GAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGT118190.15050306914711276No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA117610.149764497524257No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT116010.14772705856465482No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA114770.14614804337096313No Hit
AAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA113650.1447218360992416No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT113580.14463269814475901No Hit
CTTAAATACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAA111590.1420986334387538No Hit
ACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTA111310.14174208162082344No Hit
TTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCT111180.14157653970535575No Hit
AATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGC109030.13883873110339034No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT108680.13839304133097738No Hit
TACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGT108040.13757806574713652No Hit
CCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATT107800.13727244990319618No Hit
TTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAA107690.13713237597472355No Hit
GGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTAT107230.1365466122738379No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT106960.13620279444940506No Hit
TGCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC106530.13565523272901195No Hit
AAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTATATACA106420.1355151588005393No Hit
ACCAAAATGGAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC102960.1311091970503996No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCT102650.1307144432519767No Hit
GTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGG102320.13029422146655875No Hit
GTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGT102060.1299631376356234No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA101640.12942830990872783No Hit
GGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCC101620.1294028419217328No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC101460.1291990980257726No Hit
ATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACT99980.12731446698814056No Hit
GAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTC98380.1252770280285384No Hit
ATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAA98130.12495867819110058No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG97940.1247167323146478No Hit
GAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGG97770.12450025442519008No Hit
TGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTG97490.1241437026072597No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTTCCATTGCGAA93840.11949579498066726No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT90880.11572653290540323No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT90640.11542091706146292No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA89050.11339621209535825No Hit
GTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCT89030.11337074410836323No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC88760.11302692628393037No Hit
GACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCA86280.109868895896547No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA86250.10983069391605445No Hit
GAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTACCCC85870.10934680216314893No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT85850.10932133417615392No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA85240.10854456057280558No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC84000.1069655453791139No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT83020.10571761401635758No Hit
CTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAAC82540.10510638232847692No Hit
TTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTG82520.10508091434148191No Hit
GTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATT82120.10457155460158135No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT80830.1029288694404021No Hit
ATCTACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATG80310.10226670177853141No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTT79090.10071315457183476No Hit
ATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTT78630.10012739087094913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)