FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_E10d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_E10d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16499117
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC1748771.0599173276969913No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT1418440.8597066133902802No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC1203880.7296632904657868No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA1062070.6437132362901603No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG1019350.6178209415691761No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA1002990.6079052594147917No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC948640.574964102624401No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT918210.5565206913800297No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA896700.5434836300633543No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA799090.48432288830971987No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG725590.43977504977993676No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG724460.4390901646433564No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG691830.41931334870829756No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA648880.3932816525878324No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC573140.34737616564571305No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA549690.3331632838290679No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA544810.33020554978790684No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG534270.3238173291334318No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA529890.32116264161288144No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA490560.29732500230163833No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT481800.29201562726053765No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT456480.27666935145680827No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA453550.27489349884602915No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT447570.2712690624595243No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC439730.26651729301634747No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT416780.2526074577203131No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA409210.2480193333982661No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG407300.24686169568953298No Hit
GCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA372520.2257817797158478No Hit
CATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGATA369550.22398168338341987No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA362890.21994510372888443No Hit
CACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGA358940.2175510362160593No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG353000.21395084355120336No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA350850.2126477435125771No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA337110.20432002512619313No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC331930.20118046317266555No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT325870.19750753934286303No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT319680.19375582341770167No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT314870.19084051588942608No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA314120.19038594610850992No Hit
GTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACA305840.18536749572719557No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA301930.18299767193601937No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC297780.18048238581494996No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA293650.17797922155470502No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA290680.17617912522227705No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC289250.17531241217333024No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA288810.17504573123519276No Hit
TAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAA284480.1724213483667035No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA282750.17137280740539024No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC280280.16987575759357304No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA274970.16665740354468667No Hit
AGTTATCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAG272570.16520278024575497No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG269750.16349359786951023No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC257630.15614775020990518No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC254710.15437795852953828No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA254660.1543476538774772No Hit
ATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGTGC253220.15347487989811817No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG253180.1534506361764693No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC252310.15292333523060658No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT251340.1523354249806217No Hit
GGTCATTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTT249920.1514747728620871No Hit
AAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT249530.1512383965760107No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT244550.14822005323072743No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT244230.14802610345753656No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA239390.1450926131380243No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG237340.14385012240352013No Hit
GGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATG237200.1437652693777491No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC233060.14125604418709195No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA232990.14121361767420643No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT228970.13877712364849584No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG228500.13849225991912173No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG228060.13822557898098425No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT227460.13786192315625134No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG225060.13640729985731964No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC224060.1358012068160981No Hit
TTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCT223780.13563150076455607No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG222950.1351284435403422No Hit
CCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATT222940.13512238260992998No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT221610.13431627886510533No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTT221560.13428597421304425No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT220310.13352835791151732No Hit
AGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTG216430.13117671691157776No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT215100.1303706131667531No Hit
GTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTA215080.1303584913059287No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA212930.12905539126730237No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA211220.12801897216681354No Hit
ATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGTGTT211080.1279341191410425No Hit
CTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAAC209250.1268249688756071No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA209210.12680072515395824No Hit
GAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGT207570.1258067325663549No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT206140.12494001951740813No Hit
CAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCTT205220.1243824139194843No Hit
CCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTT203970.12362479761795737No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA203090.12309143574168242No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA200820.12171560453810952No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC200540.1215458984865675No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC197190.11951548679847535No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA194750.11803661977789477No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT194330.11778206070058174No Hit
GCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAGT194080.11763053744027635No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA193680.11738810022378773No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA193360.11719415045059683No Hit
CTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATA193320.11716990672894798No Hit
GTCTTTAATGGTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAG192230.1165092653140165No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG191840.1162728890279401No Hit
TCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTC191570.1161092439068103No Hit
GTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGT191500.11606681739392478No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA191440.11603045181145148No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC190210.115284957370749No Hit
GTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGG189510.11486069224189391No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC188900.11449097548674878No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT187540.11366668895068748No Hit
TGTTAACATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGTAATGACG185960.11270906194555746No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG184160.11161809447135868No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT183740.11136353539404563No Hit
GTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATAT180990.10969677953068639No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA179600.10885431020338845No Hit
GTATATAGCCCATCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATT179080.10853914182195326No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT178800.10836943577041122No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG177160.10737544318280791No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC176930.10723604178332696No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC172640.10463590263648656No Hit
AATGGAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG172000.10424800309010478No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG170590.1033934119019824No Hit
CATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATAT169740.10287823281694408No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA168700.10224789605407368No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA167810.10170847324738651No Hit
TGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTG167460.10149634068295899No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT167070.10125996439688256No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC167030.1012357206752337No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA166970.10119935509276042No Hit
CATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATC166500.1009144913633863No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA165600.10036900762628691No Hit
GGGCTACCCCTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGA165570.10035082483505027No Hit
TTTCTATTCTGAAGATCTTGTATGAGGCCTGTCCATCACTTGGTCCATCG165120.10007808296650057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)