FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_D2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_D2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4813706
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC502981.04489139968249No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT475800.988427627279273No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC406000.8434250035211955No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC404990.841326828019825No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC346780.7204012874903453No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC311100.6462796024518322No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT304730.6330465549827929No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT301220.6257548757651589No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA291710.6059987876284925No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG264750.5499920435523067No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA249770.5188725692844557No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT244750.5084440138221985No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA241880.5024818715559279No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA240600.4998227976532011No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA238090.4946085199220725No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC233550.48517711717333795No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT231400.48071070397735133No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT231240.48037831973951045No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA230230.47828014423814No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA229570.4769090592570464No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT226420.47036524457455436No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA223630.46456929442720435No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC219870.456758264837944No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG218400.453704484652781No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT217890.4526450098946633No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC207400.4308530683012216No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA203560.4228758465930408No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT197020.40928964087129543No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC189550.3937714517671No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT185070.3844646931075558No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG184420.3831143821413273No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC181050.37611353913180406No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC178940.37173022199527767No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC176770.36722226076956094No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC170680.354570885716743No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC169670.3524727102153725No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG167870.3487333875396628No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA163110.33884495646389706No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA161550.3356042101449486No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA160910.33427467319358517No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA159020.3303483843840899No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT153720.31933815650561126No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA153680.31925506044615104No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG152380.316554438513694No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG149360.3102806860244477No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA147990.3074346459879353No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA145400.30205417613788627No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG145340.30192953204869594No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT145200.30163869584058517No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT144390.2999560006365158No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG142180.2953649433513389No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA139450.2896936372931791No Hit
GGTAAATCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTT138900.2885510664756011No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA137080.28477019577016127No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA136000.28252660216473546No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG134500.27941049993497735No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG134450.279306629860652No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT133410.27714613231468643No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA132890.27606588354170364No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT132060.27434164030790414No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG129880.26981290506732236No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG128340.26661370677810403No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA127750.2653880399010658No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA124300.25822100477262216No Hit
ACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTG124080.25776397644559096No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG123360.25626824737530707No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT121540.25248737666986726No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA121330.2520511223577011No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA119120.24746006507252416No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT118580.24633826826981126No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA118120.24538266358601876No Hit
GACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAG113270.23530726637646754No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA110360.22926202805073678No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG108380.22514877310745607No Hit
ACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTA108350.22508645106286093No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTA106950.22217808898175334No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT106710.22167951262499203No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT106350.2209316480898501No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT105560.21929050091551083No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT102300.21251817206950321No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA101980.21185340359382146No Hit
GGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCC101660.21118863511813976No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT100860.20952671392893543No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA100290.20834259508162734No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA100180.20811408091811176No Hit
TGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAA99990.2077193746356757No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT97650.20285825515725303No Hit
AATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGC97430.20240122683022185No Hit
ATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCC97190.2019026504734606No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT94430.19616902237070566No Hit
TACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGT93580.19440323110717606No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG92510.19218041151661527No Hit
TGCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC88710.18428628586789472No Hit
GTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGG86660.18002761282055862No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT84810.17618442007052362No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA83960.17441862880699402No Hit
GACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCA81520.16934976917992084No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC81330.1689550628974848No Hit
CTTAAATACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAA81300.16889274085288966No Hit
TTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAA80180.1665660511880036No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA79910.16600515278664713No Hit
ATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACT78840.16378233319608634No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG77940.16191267185823147No Hit
AAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA77800.16162183565012073No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC76920.15979372234199596No Hit
ATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAA76660.15925359795550456No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC76420.15875502159874327No Hit
GAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGG74780.15534808316087437No Hit
CATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAAC74730.15524421308654912No Hit
TATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGA73250.1521696588865211No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA73220.15210733684192596No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG72680.15098554003921302No Hit
GAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTC72130.14984296922163506No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG72130.14984296922163506No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT72030.14963522907298452No Hit
CGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAA71460.14845111022567642No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT70700.1468722850959323No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTATT70470.1463944827540361No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA69910.14523113792159306No Hit
GAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTACCCC69870.14514804186213284No Hit
AATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTATATACAT69830.14506494580267262No Hit
TTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTG69450.14427553323780054No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT69130.14361076476211881No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAAATTAC68290.1418657475134543No Hit
CTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCA65970.13704617606476174No Hit
CAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCAGTTCA64880.13478180844447085No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG64620.13424168405797945No Hit
TGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTG63690.1323097006755294No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC63660.13224737863093425No Hit
TCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATT63400.13170725424444285No Hit
AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAAT63300.1314995140957923No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG63100.1310840337984912No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG62940.13075164956065036No Hit
CAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTAC62580.1300037850255084No Hit
CGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAA62000.1287988921633353No Hit
ATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAAT61510.12778096543494763No Hit
ATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTG59110.12279520186733466No Hit
GTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATT58060.12061393030650396No Hit
CACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT57810.12009457993487761No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT57630.11972064766730664No Hit
GTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGT56920.11824569261188782No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC56360.11708234777944478No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA55870.11606442105105712No Hit
CATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGG55790.11589822893213671No Hit
CTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATT55460.11521268644158991No Hit
GAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATC55340.11496339826320925No Hit
GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACACGT54870.11398701956455172No Hit
GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGA54810.1138623754753614No Hit
GAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACA54450.11311451094021945No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT54190.11257438655372803No Hit
GGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCA54170.11253283852399794No Hit
CTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACT54090.1123666464050775No Hit
ATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATC53630.11141104172128502No Hit
GTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCT53220.1105593071118178No Hit
CATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTT53030.11016460082938176No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA52940.1099776346955963No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT52820.10972834651721564No Hit
TTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAG52070.10817029540233658No Hit
GTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTA51600.10719391670367903No Hit
GAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGG51160.10627986004961666No Hit
GTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT51160.10627986004961666No Hit
GATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAA50740.10540735142528439No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTTCCATTGCGAA50370.10463871287527739No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCT50360.10461793886041232No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT49840.10353769008742952No Hit
TGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAG49370.10256131138877199No Hit
GGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAA48720.10121100042254345No Hit
CGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCT48360.10046313588740151No Hit
TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAA48340.10042158785767141No Hit
GAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAAT48270.10027616975361603No Hit
GGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTAGTTCTGCTAT48210.1001515256644257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)