FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_A11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_R1_A11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5214771
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC458140.8785428928710388No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC401620.7701584594989885No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT346330.6641327107173066No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT304830.5845510761642265No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT303760.5824992123335809No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC281630.5400620660044324No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG280420.5377417340090294No Hit
GTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGT253870.4868286641925407No Hit
GTTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT248040.47564888276014416No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC242750.4655046213918118No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC239430.45913809062756544No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA227950.4371237011174604No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG216860.4158571872091794No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA215650.41353685521377637No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT211350.4052910472962284No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT210830.4042938798271295No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC209420.4015900218820731No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA201690.3867667439279692No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA199570.3827013688616432No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATC191340.36691927603340585No Hit
GGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA182380.34973731348893367No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG175450.3364481393334434No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC175280.336122142276238No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA171700.3292570277774422No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC170770.3274736321115539No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA170050.32609293869280165No Hit
ACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAG169540.32511494752118547No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT167340.3208961620749981No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT161850.31036837475701234No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA159000.3049031299744514No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG158460.30386760991038725No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC157960.30290879503625373No Hit
GTTTTTTAGCTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT151050.28965797347572886No Hit
GTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA146770.28145051815314615No Hit
GAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAA137670.2640000874439165No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG137550.2637699718741245No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT134030.2570199151602247No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA132780.2546228779748909No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT131050.2513053785103891No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC130050.24938774876212205No Hit
GAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTT128770.2469331826843403No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT128650.24670306711454829No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC128310.24605107300013748No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG126870.24328968616263302No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC122380.23467952859291424No Hit
ACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTA121940.23383577150367676No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG121930.2338165952061941No Hit
GAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGT121280.23257013586982056No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC118220.22670218884012355No Hit
GAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATT116800.22397915459758444No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA116280.2229819871284856No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG116050.2225409322863842No Hit
ATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCC115800.22206152484931743No Hit
CTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA113420.21749756604844203No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG111620.21404583250156142No Hit
ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG111570.21394995101414807No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT109460.20990375224530472No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA109010.20904081885858458No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA107400.20595343496387472No Hit
ACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAACTG104040.19951019900969766No Hit
TGCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC103540.19855138413556414No Hit
CTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAA103400.19828291597080677No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA102500.19655704919736647No Hit
ATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTAGCTGGCTT102190.19596258397540373No Hit
GGTAAATCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTT97650.1872565449182716No Hit
GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAA93680.17964355481765162No Hit
GACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAG92940.17822450880393406No Hit
GGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCC92220.17684381538518182No Hit
TACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGT91370.1752138300991549No Hit
AAATTAAACAGAGAAGAAATAGATGGGGTAAAACTGGAATCAACAAGGAT90520.17358384481312794No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC90400.17335372924333592No Hit
TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACT89870.1723373854767544No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT89320.17128268911520755No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA87560.16790766075825767No Hit
AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAAT87180.1671789614539162No Hit
ACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTA85430.16382310939444894No Hit
GTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGG83890.16086995958211778No Hit
TGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAAA83670.16044808103749905No Hit
CAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCAGTTCA83120.1593933846759522No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT81890.15703470008558382No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG80050.15350626134877254No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA77890.14936418109251587No Hit
AAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAA77590.14878889216803576No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC75650.1450686904563978No Hit
AAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA73680.14129095985231183No Hit
AATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGC72700.13941168269901016No Hit
CGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAA71980.13803098928025795No Hit
ATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATC71880.13783922630543124No Hit
CATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAACCAAC71870.13782005000794859No Hit
ATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAAT71730.1375515818431912No Hit
TTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAA71670.13743652405829518No Hit
CTTAAATACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAA71450.13701464551367645No Hit
GAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAAAATGG70100.134425845353516No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT70080.13438749275855066No Hit
CTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCA69950.13413820089127595No Hit
GACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCA68300.1309741118066354No Hit
CACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT67420.12928659762816047No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA66830.12815519607668296No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT66820.12813601977920028No Hit
CTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATT66790.12807849088675224No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG66730.12796343310185623No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA66660.12782919901947756No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC64600.12387888173804756No Hit
GTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAA63960.12265159869915669No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA63540.12184619420488454No Hit
GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACACGT63140.12107914230557776No Hit
GGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAA62480.11981350667172154No Hit
ATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACTTATATGAA62260.11939162812710281No Hit
GTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT61980.11885469179758804No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG61580.11808763989828125No Hit
CGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAA61580.11808763989828125No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT61450.11783834803100654No Hit
GAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAAT60600.1162083627449796No Hit
GTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGT59550.11419485150929926No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA59430.11396473593950722No Hit
TTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAG59030.11319768404020043No Hit
TGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTT58990.11312097885026974No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA58160.11152934615920815No Hit
GGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCA57960.11114582020955474No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA57610.1104746497976613No Hit
GAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTC56680.108691254131773No Hit
ATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTG56390.10813514150477556No Hit
GTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATT56330.10802008371987955No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA56040.10746397109288212No Hit
CAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTAC55790.10698456365581537No Hit
GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGA54950.10537375466727111No Hit
ATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATGTGAAGAACT54520.1045491738755163No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC54260.10405059014096689No Hit
TGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTG53610.10280413080459333No Hit
CGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCT53570.10272742561466267No Hit
CATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGG52920.1014809662782891No Hit
TTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAA52660.1009823825437397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)