FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n01_CIVIR_Ferret_Flu_Control_1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n01_CIVIR_Ferret_Flu_Control_1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10069022
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG500690.49725782702629906No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC431760.4288003343323711No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC408930.40612683138441846No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA349100.34670695922603006No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG346130.3437573182380573No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT306580.3044784289874429No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA292330.29032611111585616No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA292190.2901870707999248No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC288350.28637339356294983No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG278590.27668029725230514No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG272320.270453277388807No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA270430.26857623312373335No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA255340.25358967335655835No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT241540.23988427078617966No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA239910.23826544425069288No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC232850.23125383974729619No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT225820.2242720295973134No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA221270.21975321932954364No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC213860.21239401403631855No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG208180.20675294978995973No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC205050.20364440558377964No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT203760.20236324838698336No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA203600.20220434516877608No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA200920.199542716263804No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA198900.19753656313393694No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC196870.19552047855293195No Hit
GTCTTTAATGGTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAG192660.19133933762385263No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA191670.19035612396119503No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA188470.18717805959704925No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA188120.1868304588072208No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG186990.18570820482863182No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC186140.18486403148190558No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC185520.18424828151135234No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA184940.18367225734535092No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC183830.18256986626903784No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT179820.17858735436271767No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC178130.17690893912040315No Hit
GTATATAGCCCATCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATT177480.17626339479643605No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA177130.1759157940066076No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC176300.17509148356215726No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA176110.1749027859905361No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT174810.17361169734260187No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC174040.1728469756049793No Hit
CATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGATA173610.1724199232060472No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA173170.17198293935597717No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC172270.17108910875356118No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG168830.16767268956210446No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA168750.1675932379530008No Hit
CACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGA160290.15919123029029036No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA158880.15779089567983862No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC157350.15627138365573143No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG154840.15377858942010456No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA153280.15222928304258349No Hit
TGTTAACATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGTAATGACG152600.1515539443652025No Hit
GGTCATTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTT146850.14584335996087802No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC146780.14577383980291234No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG146630.145624868035843No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC145940.14493959790732408No Hit
CATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATC143340.14235742061145562No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT143020.14203961417504102No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC140800.1398348320224149No Hit
GCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA139920.13896086432227478No Hit
GTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGG138490.13754066680954713No Hit
TCTCAAAACCGTTTCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA136620.13568348544674946No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT136480.13554444513081806No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC134110.1331906912111226No Hit
GTTTTTTAACTGGCTTCTTACCTTTTCATATAAGTTCTTCACATTTGAAT133690.13277357026332845No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT133140.13222734045074092No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA132260.1313533727506008No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA131990.13108522356987598No Hit
GAATAGAAAAGGGAAAGATAGTCAAATCAGTCGAAATGAATGCCCCTAAT131410.1305091994038746No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC130960.13006228410266657No Hit
AGTTATCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAG130730.1298338607264936No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC128910.12802633661938567No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT128350.12747017535566016No Hit
GCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAGT128080.12720202617493537No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG127540.12666572781348576No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG126740.1258712117224493No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAAC126520.1256527197974143No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT126270.1254044335189654No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA122610.12176952240247364No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT122570.12172979659792182No Hit
CAATTCAACTTGGGAATCAAAATCAGATTGAAACATGCAATCAAAGCGTC120970.12014076441584892No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT119890.11906816769294971No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT119800.11897878463270813No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG119770.11894899027929426No Hit
GCTCATTAGGGTTCGATATGGGCTCCTGTCTTTAATGGTTCCATTGGAAT118140.11733016374380749No Hit
GTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACA114240.1134568978000048No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTT113930.11314902281472819No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG113110.11233464382141582No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA112070.11130177290306843No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT111430.11066616003023927No Hit
TTCCAAGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCC111010.11024903908244514No Hit
GGTAAGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT110580.10982198668351305No Hit
CTAATGAGCTGTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATT110550.10979219233009918No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT109900.10914664800613207No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA109620.10886856737426932No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC109220.1084713093287511No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA109070.10832233756168175No Hit
ATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGTGC108830.10808398273437082No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA108580.10783569645592193No Hit
GAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC107450.10671344247733296No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG106590.10585933767946877No Hit
TAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAA106500.10576995461922718No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC106290.10556139414533011No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT106120.10539255947598486No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA105500.10477680950543161No Hit
CAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCTT104380.10366448697798059No Hit
GTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCA103840.10312818861653097No Hit
GTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATTTGAGTCAGTC103830.10311825716539302No Hit
CCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTT103810.10309839426311711No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA103780.10306859990970324No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT103510.10280045072897845No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA103410.10270113621759888No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA102560.10185696287087266No Hit
TCCCAAATCATCTCAAAACCGTTTCTTGAACTAATGCTTTTAGTTCTCCC101480.10078436614797345No Hit
TCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATTTCACGGAAACCA101370.10067512018545595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)