FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_wt0_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_wt0_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11305136
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG42900.047.04379350
TTAGGGG39700.041.5370249
TTTAGGG53000.031.83155448
GGGGAAT62950.030.6936163
TTGGGGG84750.028.98304650
AGGGGGG75750.028.60109551
TATAGGG13000.027.3349848
TTTGGGG87550.027.18885449
ATAGGGG13750.026.84596649
TTTTGGG92900.026.6645148
AAGGGGG31650.024.96131551
TAAGGGG27200.023.46424350
CACCTAG12150.021.8663275
GGGGAAA147450.021.5251822
GGGGAGA44300.021.41572222
GGGGGAT37550.021.13360263
GGGGGTA29500.020.46791665
GGGGGAA167000.020.2665362
GGGGTAA29450.019.79936466
GGGGGGA223800.019.299520
Produced by FastQC (version 0.11.2)