FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_wt-1_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_wt-1_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487068
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG29150.053.0201350
TTAGGGG25600.047.300849
TATAGGG7550.042.95321348
TTTAGGG33200.035.95445648
GGGGAAT38700.035.8385163
ATAGGGG9050.035.45158849
AGGGGGG49050.033.90101651
TTGGGGG63600.033.52221750
TTTGGGG61000.033.1412749
TTTTGGG65350.032.15040648
TAAGGGG13800.029.99877450
GGGGAGA27900.029.67620322
TTATAGG11400.027.53962147
GAGAGGG11900.027.24930425
AAGGGGG18350.024.44060751
GGGAGAG20600.024.28242523
TGGGGGG94250.023.06024251
CGGGGAG11100.023.0132541
TGGGGGA14450.022.68073320
GGGGAAA80650.022.67111222
Produced by FastQC (version 0.11.2)