FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_vgt2_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_vgt2_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195457
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA91470.1476404404065753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG26350.036.265950
TTAGGGG23650.032.8209149
TTTGGGG50300.029.42311149
TTGGGGG55150.028.64964550
TTTTGGG54150.027.20440348
GGGGAAT35000.025.6300963
GGGGGTA14550.024.89858665
GGTTGGT10700.024.17957931
TTTAGGG33350.023.3788148
GGGGTAA15600.022.33786266
GATTCGA30250.022.2438624
GTTTGGT20200.022.20052331
AGGGGGG50650.022.0003451
TGGGGGG74800.021.4465651
GGGGAAA76000.021.3801222
GTACCCC1507.305716E-720.7109261
GTGATAG16850.020.2738514
CCTAACT5800.020.2300742
ACTAGAT27650.020.21278852
ATTCGAC34200.019.976485
Produced by FastQC (version 0.11.2)