FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_vgt1_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_vgt1_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7035611
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGT11950.027.716049
TAGGGGG18800.025.87386550
CGATTAG14100.023.0014448
TTTGGGG41300.022.13551749
TTAGTAT14800.021.91182311
TTGGGGG44800.021.7925750
CTAGACT1304.929305E-621.22998851
TTAGGGG18300.020.17095849
TTTTGGG45350.019.93161848
GGTTGGT9550.019.8673931
GGGGAAT24950.019.63566463
GGGGTTA10100.019.47110465
TGGGGGG55400.018.74389651
CCTAACT7050.018.6016712
AGGGGGG33050.018.26710351
GGGGGTA13550.018.0782465
GGGGGGA68400.017.55198520
ATTAGTA19150.017.29538510
GATTCGA22400.017.0996074
GGGGGAT17750.016.13271963
Produced by FastQC (version 0.11.2)