FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_vgt0_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_vgt0_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7496823
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG19550.027.70415950
TTAGGGG19100.022.93775249
GATTAGT12900.022.7332679
TTGGGGG46950.022.11683350
TTTGGGG42850.021.89770149
GGGGAAT28250.021.739663
TTTTGGG44350.021.3140748
CGATTAG14250.021.0642348
TTAGTAT15350.019.1041911
ATTAGTA15950.018.81826410
TGGGGGG57250.018.74031851
GGGGGGA74000.018.5082520
AGGGGGG34800.018.24024251
GGTTGGT12350.017.87659531
GGGGGAA59350.017.43888362
GGGGAAA59100.017.3368322
GTCTAAC8300.017.0527111
GGGGGAT17500.016.75833763
GGGGGTA12200.016.68594765
CCTAACT7350.016.4353332
Produced by FastQC (version 0.11.2)