FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_sigg45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_sigg45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886866
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGG200.00735835551.7531977
GATTAGT6500.027.6002719
TTAGTAT7350.025.81568511
CGATTAG7300.024.5764338
ATTAGTA9200.020.24949610
CGATCGA10200.019.285284
TAGTCTA1301.1804677E-418.5796869
GACCTAT9450.018.25256553
CTAACTG4550.017.4488011
TCGATCG11300.017.103443
ACGCACG8300.016.21044210
GTGTCGC10550.016.0219131
GCCACAC12750.015.96375636
AGTATCT11700.015.62781613
CCTCGAT12900.015.5198461
TCGCCAC10900.015.50798334
GATCGAT13400.015.4511255
TAGGGGT7600.015.43355944
GGGTAGG8150.015.23835741
CTAGCCC6350.015.21143134
Produced by FastQC (version 0.11.2)